{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T09:01:30Z","timestamp":1789894890347,"version":"4.0.1"},"reference-count":42,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T00:00:00Z","timestamp":1494288000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Twin Res Hum Genet"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:p>Massively parallel sequencing (MPS) technology has become increasingly available\n                    and has been widely used to screen for trisomies 21, 18, and 13 in singleton\n                    pregnancies. This study assessed the performance of MPS testing of cell-free\n                    fetal DNA (cffDNA) from maternal plasma for trisomies 21, 18, and 13 in twin\n                    pregnancies. Ninety-two women with twin pregnancies were recruited. The results\n                    were identified through karyotypes of amniocentesis or clinical examination and\n                    follow-up of the neonates. Fluorescent in-situ hybridization was used to examine\n                    the placentas postnatally in cases of false-positive results. The fetuses with\n                    autosomal trisomy 21 (<jats:italic>n<\/jats:italic> = 2) and trisomy 15 (<jats:italic>n<\/jats:italic>\n                    = 1) were successfully detected via MPS testing of cffDNA. There was one\n                    false-positive for trisomy 13 (<jats:italic>n<\/jats:italic> = 1), and fluorescence in-situ\n                    hybridization (FISH) identified confined placental mosaicism in this case. For\n                    twin pregnancies undergoing second-trimester screening for trisomy, MPS testing\n                    of cffDNA is feasible and can enhance the diagnostic spectrum of non-invasive\n                    prenatal testing, which could effectively reduce invasive prenatal diagnostic\n                    methods. In addition to screening for trisomy 21, 18, and 13 by cffDNA, MPS can\n                    detect fetal additional autosomal trisomy. False-positive results cannot\n                    completely exclude confined placental mosaicism.<\/jats:p>","DOI":"10.1017\/thg.2017.23","type":"journal-article","created":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T06:09:40Z","timestamp":1494310180000},"page":"242-249","source":"Crossref","is-referenced-by-count":20,"title":["Massively Parallel Sequencing (MPS) of Cell-Free Fetal DNA (cffDNA)\n                    for Trisomies 21, 18, and 13 in Twin Pregnancies"],"prefix":"10.1017","volume":"20","author":[{"given":"Erqiu","family":"Du","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuming","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanru","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanzhen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2017,5,9]]},"reference":[{"key":"S1832427417000238_ref036","doi-asserted-by":"publisher","DOI":"10.1002\/uog.8978"},{"key":"S1832427417000238_ref009","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.c7401"},{"key":"S1832427417000238_ref006","doi-asserted-by":"publisher","DOI":"10.1002\/pd.3892"},{"key":"S1832427417000238_ref002","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1700"},{"key":"S1832427417000238_ref033","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.32729"},{"key":"S1832427417000238_ref022","doi-asserted-by":"publisher","DOI":"10.1159\/000346028"},{"key":"S1832427417000238_ref028","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4132"},{"key":"S1832427417000238_ref015","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1448.050"},{"key":"S1832427417000238_ref027","doi-asserted-by":"publisher","DOI":"10.3109\/14767058.2012.733768"},{"key":"S1832427417000238_ref014","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh117"},{"key":"S1832427417000238_ref030","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2014.03.011"},{"key":"S1832427417000238_ref011","doi-asserted-by":"publisher","DOI":"10.1053\/j.semperi.2006.01.002"},{"key":"S1832427417000238_ref004","doi-asserted-by":"publisher","DOI":"10.1002\/uog.14690"},{"key":"S1832427417000238_ref039","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2009.11.016"},{"key":"S1832427417000238_ref021","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4303"},{"key":"S1832427417000238_ref031","doi-asserted-by":"publisher","DOI":"10.1111\/ajo.12071"},{"key":"S1832427417000238_ref020","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmt006"},{"key":"S1832427417000238_ref038","doi-asserted-by":"publisher","DOI":"10.1002\/uog.15913"},{"key":"S1832427417000238_ref037","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2013.01.001"},{"key":"S1832427417000238_ref032","doi-asserted-by":"publisher","DOI":"10.1097\/01.OGX.0000082369.30519.A8"},{"key":"S1832427417000238_ref010","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-013-1998-3"},{"key":"S1832427417000238_ref041","doi-asserted-by":"publisher","DOI":"10.1053\/j.semperi.2012.02.008"},{"key":"S1832427417000238_ref024","first-page":"723","article-title":"Confined 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