{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T11:06:13Z","timestamp":1772795173708,"version":"3.50.1"},"reference-count":17,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T00:00:00Z","timestamp":1502668800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Twin Res Hum Genet"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:p><jats:italic>CDKN1C<\/jats:italic> and <jats:italic>KCNQ1OT1<\/jats:italic> are imprinted genes that might be potential regulators of placental development. This study investigated placental expressions of <jats:italic>CDKN1C<\/jats:italic> and <jats:italic>KCNQ1OT1<\/jats:italic> in monozygotic twins with and without selective intrauterine growth restriction (sIUGR). Seventeen sIUGR and fifteen normal monozygotic(MZ) twin pairs were examined. Placental mRNA expressions of <jats:italic>CDKN1C<\/jats:italic> and <jats:italic>KCNQ1OT1<\/jats:italic> were detected by real-time fluorescent quantitative PCR. <jats:italic>CDKN1C<\/jats:italic> protein expression was detected by immunohistochemical assay and Western-blotting. In the sIUGR group, smaller fetuses had a smaller share of the placenta, and <jats:italic>CDKN1C<\/jats:italic> protein expression was significantly increased while <jats:italic>KCNQ1OT1<\/jats:italic> mRNA expression was significantly decreased. The <jats:italic>CDKN1C<\/jats:italic>\/<jats:italic>KCNQ1OT1<\/jats:italic> mRNA ratio was lower in the larger fetus than in the smaller fetus (<jats:italic>p<\/jats:italic> &lt; .05). In the control group, <jats:italic>CDKN1C<\/jats:italic> protein expression showed no difference between larger and smaller fetuses, while <jats:italic>KCNQ1OT1<\/jats:italic> mRNA expression was significantly lower in the larger fetus, and the <jats:italic>CDKN1C<\/jats:italic>\/<jats:italic>KCNQ1OT1<\/jats:italic> mRNA ratio was higher in the larger fetus than in the smaller fetus (<jats:italic>p<\/jats:italic> &lt; .05). Our findings showed that pathogenesis of sIUGR may be related to the co-effect of the up-regulated protein expression of <jats:italic>CDKN1C<\/jats:italic> and down-regulated mRNA expression of <jats:italic>KCNQ1OT1<\/jats:italic> in the placenta.<\/jats:p>","DOI":"10.1017\/thg.2017.41","type":"journal-article","created":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T03:02:29Z","timestamp":1502679749000},"page":"389-394","source":"Crossref","is-referenced-by-count":11,"title":["Placental Expressions of <i>CDKN1C<\/i> and <i>KCNQ1OT1<\/i> in Monozygotic Twins with Selective Intrauterine Growth Restriction"],"prefix":"10.1017","volume":"20","author":[{"given":"Chenyu","family":"Gou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangzhen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomei","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanjing","family":"Chai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-ming","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qun","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2017,8,14]]},"reference":[{"key":"S183242741700041X_ref012","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-09-0317"},{"key":"S183242741700041X_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2006.10.865"},{"key":"S183242741700041X_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2007.04.001"},{"key":"S183242741700041X_ref007","first-page":"1","article-title":"Placental characteristics in monochorionic twins with selective intrauterine growth restriction assessed by gradient angiography and three-dimensional reconstruction","volume":"21","author":"Gou","year":"2017","journal-title":"Journal of Maternal-Fetal and Neonatal Medicine"},{"key":"S183242741700041X_ref008","doi-asserted-by":"publisher","DOI":"10.1002\/uog.4046"},{"key":"S183242741700041X_ref001","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-7-53"},{"key":"S183242741700041X_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2010.07.002"},{"key":"S183242741700041X_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2005.07.004"},{"key":"S183242741700041X_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2011.02.002"},{"key":"S183242741700041X_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2010.02.018"},{"key":"S183242741700041X_ref006","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1001015"},{"key":"S183242741700041X_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2013.10.007"},{"key":"S183242741700041X_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2004.03.008"},{"key":"S183242741700041X_ref002","doi-asserted-by":"publisher","DOI":"10.1097\/PAT.0b013e3283559c94"},{"key":"S183242741700041X_ref004","doi-asserted-by":"publisher","DOI":"10.4161\/epi.9019"},{"key":"S183242741700041X_ref011","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmq052"},{"key":"S183242741700041X_ref014","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2010.01373.x"}],"container-title":["Twin Research and Human Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S183242741700041X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T22:31:50Z","timestamp":1555453910000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S183242741700041X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,14]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["S183242741700041X"],"URL":"https:\/\/doi.org\/10.1017\/thg.2017.41","relation":{},"ISSN":["1832-4274","1839-2628"],"issn-type":[{"value":"1832-4274","type":"print"},{"value":"1839-2628","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,14]]}}}