{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:01:24Z","timestamp":1758819684358},"reference-count":49,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":5058,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1995,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The proteolytic activities present in adult<jats:italic>Necator americanus<\/jats:italic>excretory\u2013secretory products have been assessed using biologically relevant, naturally occurring substrates (haemoglobin and fibrinogen) and a number of synthetic fluorogenic and chromogenic substrates. One broad peak of activity was observed against haemoglobin in the pH range 5 to 7, with maximum activity at pH 6\u00b76, while fibrinogenolytic activity was shown to be greater at pH 3\u00b75. Inhibition studies against haemoglobin, fibrinogen and synthetic substrates using a battery of appropriate protease inhibitors indicated the presence of a mixture of aspartyl, cysteinyl and serine proteases. Metal ion (Ca<jats:sup>2+<\/jats:sup>, Zn<jats:sup>2+<\/jats:sup>and Fe<jats:sup>2+<\/jats:sup>) stimulation was demonstrated, with stimulation by Zn<jats:sup>2+<\/jats:sup>being the most marked. These results are discussed in the context of recent developments in the field of parasite proteolytic enzymes, where they have been suggested as targets for immuno- and chemotherapy.<\/jats:p>","DOI":"10.1017\/s0031182000065276","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T13:48:39Z","timestamp":1244209719000},"page":"555-563","source":"Crossref","is-referenced-by-count":31,"title":["An initial characterization of the proteolytic enzymes secreted by the adult stage of the human hookworm<i>Necator americanus<\/i>"],"prefix":"10.1017","volume":"110","author":[{"given":"A.","family":"Brown","sequence":"first","affiliation":[]},{"given":"J. M.","family":"Burleigh","sequence":"additional","affiliation":[]},{"given":"E. E.","family":"Billett","sequence":"additional","affiliation":[]},{"given":"D. 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