{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T12:59:43Z","timestamp":1775134783211,"version":"3.50.1"},"reference-count":60,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2010,7,2]],"date-time":"2010-07-02T00:00:00Z","timestamp":1278028800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2010,11,28]]},"abstract":"<jats:p>Different diets have distinct impacts on glucose homoeostasis, for which insulin sensitivity (IS) after a meal (postprandial IS) is highly relevant. Postprandial IS depends upon hepatic parasympathetic activation and glutathione content elevation. We tested the hypothesis that postprandial IS is compromised in high-fat diet (HFD)-induced obesity. Sprague\u2013Dawley rats were fed a standard diet (STD, <jats:italic>n<\/jats:italic> 10), 1-week HFD (<jats:italic>n<\/jats:italic> 9) or 4-week HFD (<jats:italic>n<\/jats:italic> 8). IS was tested in postprandial state using the rapid IS test (RIST) before and after the blockade of the parasympathetic nerves (atropine, 1\u00a0mg\/kg); parasympathetic-dependent IS was obtained from the difference between control and post-atropine RIST. Fasting IS was also assessed in the STD-fed rats (<jats:italic>n<\/jats:italic> 4) and 4-week HFD-fed rats (<jats:italic>n<\/jats:italic> 3) using the RIST. Whole-body fat and regional fat pads were heavier in the 1-week HFD-fed rats (79\u00b78 (<jats:sc>se<\/jats:sc> 7\u00b79) and 23\u00b77 (<jats:sc>se<\/jats:sc> 1\u00b70)\u00a0g, respectively) or 4-week HFD-fed rats (106\u00b75 (<jats:sc>se<\/jats:sc> 6\u00b71) and 30\u00b71 (<jats:sc>se<\/jats:sc> 1\u00b74)\u00a0g, respectively) than in the STD-fed rats (32\u00b75 (<jats:sc>se<\/jats:sc> 3\u00b77) and 13\u00b77 (<jats:sc>se<\/jats:sc> 1\u00b70)\u00a0g, respectively; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b7001). Fasted-state IS was similar between the groups studied. Postprandial IS was higher in the STD-fed rats (185\u00b78 (<jats:sc>se<\/jats:sc> 5\u00b76)\u00a0mg glucose\/kg body weight (bw)) than in both the 1-week HFD-fed rats (108\u00b78 (<jats:sc>se<\/jats:sc> 2\u00b79)\u00a0mg glucose\/kg bw; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b7001) and 4-week HFD-fed rats (69\u00b73 (<jats:sc>se<\/jats:sc> 2\u00b76)\u00a0mg glucose\/kg bw; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b7001). Parasympathetic-dependent IS was impaired in both HFD-fed groups (STD, 108\u00b79 (<jats:sc>se<\/jats:sc> 3\u00b79)\u00a0mg glucose\/kg bw; 1-week HFD, 38\u00b76 (<jats:sc>se<\/jats:sc> 4\u00b72)\u00a0mg glucose\/kg bw; 4-week HFD, 5\u00b74 (<jats:sc>se<\/jats:sc> 1\u00b77)\u00a0mg glucose\/kg bw; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b7001). Total (postprandial) and parasympathetic-dependent IS correlated negatively with whole-body fat (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> 0\u00b781 and 0\u00b787) and regional adiposity (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> 0\u00b785 and 0\u00b779). In conclusion, fat accumulation induced by HFD is associated with postprandial insulin resistance, but not with fasting insulin resistance. HFD-associated postprandial insulin resistance is largely mediated by impairment of parasympathetic-dependent insulin action, which correlates with adiposity.<\/jats:p>","DOI":"10.1017\/s0007114510002400","type":"journal-article","created":{"date-parts":[[2010,7,2]],"date-time":"2010-07-02T06:58:15Z","timestamp":1278053895000},"page":"1450-1459","source":"Crossref","is-referenced-by-count":18,"title":["High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunction"],"prefix":"10.1017","volume":"104","author":[{"given":"Ricardo A.","family":"Afonso","sequence":"first","affiliation":[]},{"given":"W. Wayne","family":"Lautt","sequence":"additional","affiliation":[]},{"given":"Josh","family":"Schafer","sequence":"additional","affiliation":[]},{"given":"Dallas J.","family":"Legare","sequence":"additional","affiliation":[]},{"given":"Antonio G.","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"M. 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