{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T23:12:23Z","timestamp":1776121943033,"version":"3.50.1"},"reference-count":93,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T00:00:00Z","timestamp":1684108800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["FJC2019-041291-I"],"award-info":[{"award-number":["FJC2019-041291-I"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Bull. Entomol. Res."],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mosquito surveillance programmes are essential to assess the risks of local vector-borne disease outbreaks as well as for early detection of mosquito invasion events. Surveys are usually performed with traditional sampling tools (i.e., ovitraps and dipping method for immature stages or light or decoy traps for adults). Over the past decade, numerous studies have highlighted that environmental DNA (eDNA) sampling can enhance invertebrate species detection and provide community composition metrics. However, the usefulness of eDNA for detection of mosquito species has, to date, been largely neglected. Here, we sampled water from potential larval breeding sites along a gradient of anthropogenic perturbations, from the core of an oil palm plantation to the rainforest on S\u00e3o Tom\u00e9 Island (Gulf of Guinea, Africa). We showed that (i) species of mosquitoes could be detected via metabarcoding mostly when larvae were visible, (ii) larvae species richness was greater using eDNA than visual identification and (iii) new mosquito species were also detected by the eDNA approach. We provide a critical discussion of the pros and cons of eDNA metabarcoding for monitoring mosquito species diversity and recommendations for future research directions that could facilitate the adoption of eDNA as a tool for assessing insect vector communities.<\/jats:p>","DOI":"10.1017\/s0007485323000147","type":"journal-article","created":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T08:36:41Z","timestamp":1684139801000},"page":"456-468","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":10,"title":["Monitoring mosquito richness in an understudied area: can environmental DNA metabarcoding be a complementary approach to adult trapping?"],"prefix":"10.1017","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0107-5357","authenticated-orcid":false,"given":"Rafael","family":"Guti\u00e9rrez-L\u00f3pez","sequence":"first","affiliation":[]},{"given":"Bastian","family":"Egeter","sequence":"additional","affiliation":[]},{"given":"Christophe","family":"Paupy","sequence":"additional","affiliation":[]},{"given":"Nil","family":"Rahola","sequence":"additional","affiliation":[]},{"given":"Boris","family":"Makanga","sequence":"additional","affiliation":[]},{"given":"Davy","family":"Jiolle","sequence":"additional","affiliation":[]},{"given":"Vincent","family":"Bourret","sequence":"additional","affiliation":[]},{"given":"Martim","family":"Melo","sequence":"additional","affiliation":[]},{"given":"Claire","family":"Loiseau","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2023,5,15]]},"reference":[{"key":"S0007485323000147_ref55","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.13049"},{"key":"S0007485323000147_ref44","doi-asserted-by":"crossref","first-page":"2130","DOI":"10.1038\/s41467-020-16010-4","article-title":"Accelerating invasion potential of disease vector Aedes aegypti under climate change","volume":"11","author":"Iwamura","year":"2020","journal-title":"Nature Communication"},{"key":"S0007485323000147_ref38","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.tig.2007.02.001","article-title":"DNA barcoding: how it complements taxonomy, molecular phylogenetics, and population genetics","volume":"23","author":"Hajibabaei","year":"2007","journal-title":"Trends in Genetics"},{"key":"S0007485323000147_ref42","volume-title":"Mosquitoes of the Ethiopian Region I. \u2013 Larval Bionomics of Mosquitoes and Taxonomy of Culicine Larvae","author":"Hopkins","year":"1952"},{"key":"S0007485323000147_ref47","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002590"},{"key":"S0007485323000147_ref57","doi-asserted-by":"publisher","DOI":"10.1007\/s10201-011-0362-4"},{"key":"S0007485323000147_ref66","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-8286.2007.01678.x"},{"key":"S0007485323000147_ref73","doi-asserted-by":"crossref","first-page":"e0162493","DOI":"10.1371\/journal.pone.0162493","article-title":"Detection of invasive mosquito vectors using environmental DNA (eDNA) from water samples","volume":"11","author":"Schneider","year":"2016","journal-title":"PLoS ONE"},{"key":"S0007485323000147_ref75","first-page":"1","volume-title":"Handbook to the Afrotropical Toxorhynchitine and Culicine Mosquitoes, Excepting Aedes and Culex","author":"Service","year":"1990"},{"key":"S0007485323000147_ref76","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-017-0005-3"},{"key":"S0007485323000147_ref83","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12490"},{"key":"S0007485323000147_ref12","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-019-3037-y"},{"key":"S0007485323000147_ref7","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2019.00240"},{"key":"S0007485323000147_ref84","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1111\/j.1365-294X.2011.05418.x","article-title":"Monitoring endangered freshwater biodiversity using environmental DNA","volume":"21","author":"Thomsen","year":"2012","journal-title":"Molecular Ecology"},{"key":"S0007485323000147_ref88","doi-asserted-by":"crossref","first-page":"2150","DOI":"10.1046\/j.1365-294X.2000.11142.x","article-title":"Urine collected in the field as a source of DNA for species and individual identification","volume":"9","author":"Valiere","year":"2000","journal-title":"Molecular Ecology"},{"key":"S0007485323000147_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2003.09.015"},{"key":"S0007485323000147_ref81","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2011.05317.x"},{"key":"S0007485323000147_ref72","doi-asserted-by":"publisher","DOI":"10.1016\/j.gecco.2019.e00547"},{"key":"S0007485323000147_ref39","unstructured":"Harbach, RE (2013) Mosquito taxonomic inventory. Available at http:\/\/mosquito-taxonomic-inventory info\/."},{"key":"S0007485323000147_ref3","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1021\/es404734p","article-title":"Environmental conditions influence eDNA persistence in aquatic systems","volume":"48","author":"Barnes","year":"2014","journal-title":"Environmental Science Technology"},{"key":"S0007485323000147_ref16","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-018-0192-6"},{"key":"S0007485323000147_ref52","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12643"},{"key":"S0007485323000147_ref20","first-page":"2194","article-title":"UCHIME improves sensitivity and speed of chimera detection","volume":"27","author":"Edgar","year":"2011","journal-title":"Bioinformatics (Oxford, England)"},{"key":"S0007485323000147_ref28","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2008.0118"},{"key":"S0007485323000147_ref46","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.54.110807.090611"},{"key":"S0007485323000147_ref65","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12159"},{"key":"S0007485323000147_ref60","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2009.0005"},{"key":"S0007485323000147_ref49","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1111\/j.1755-263X.2008.00011.x","article-title":"Is oil palm agriculture really destroying tropical biodiversity?","volume":"1","author":"Koh","year":"2008","journal-title":"Conservation Letters"},{"key":"S0007485323000147_ref51","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-06153-0_15"},{"key":"S0007485323000147_ref25","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv107"},{"key":"S0007485323000147_ref14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188126"},{"key":"S0007485323000147_ref10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183371"},{"key":"S0007485323000147_ref70","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1009581"},{"key":"S0007485323000147_ref37","doi-asserted-by":"publisher","DOI":"10.1111\/jvec.12127"},{"key":"S0007485323000147_ref1","unstructured":"Andrews, S (2010) FastQC: A Quality Control Tool for High Throughput Sequence Data. Available at http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"S0007485323000147_ref62","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.130"},{"key":"S0007485323000147_ref50","doi-asserted-by":"crossref","first-page":"260","DOI":"10.3389\/fevo.2019.00260","article-title":"How does eDNA compare to traditional trapping? Detecting mosquito communities in South-African freshwater ponds","volume":"7","author":"Krol","year":"2019","journal-title":"Frontiers in Ecology and Evolution"},{"key":"S0007485323000147_ref2","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/101.2.523"},{"key":"S0007485323000147_ref13","doi-asserted-by":"crossref","first-page":"e0157366","DOI":"10.1371\/journal.pone.0157366","article-title":"Spatial representativeness of environmental DNA metabarcoding signal for fish biodiversity assessment in a natural freshwater system","volume":"11","author":"Civade","year":"2016","journal-title":"PLoS ONE"},{"key":"S0007485323000147_ref45","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-263X.2010.00158.x"},{"key":"S0007485323000147_ref59","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14193.1"},{"key":"S0007485323000147_ref87","doi-asserted-by":"publisher","DOI":"10.1111\/mec.13428"},{"key":"S0007485323000147_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2013.07.003"},{"key":"S0007485323000147_ref93","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-021-04950-9"},{"key":"S0007485323000147_ref6","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.biocon.2014.11.029","article-title":"Using eDNA to develop a national citizen science-based monitoring programme for the great crested newt (Triturus cristatus)","volume":"183","author":"Biggs","year":"2015","journal-title":"Biological Conservation"},{"key":"S0007485323000147_ref68","first-page":"105","article-title":"Effects of sampling design on the estimation of adult mosquito abundance","volume":"15","author":"Reisen","year":"1999","journal-title":"Journal of the American Mosquito Control Association"},{"key":"S0007485323000147_ref19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023398"},{"key":"S0007485323000147_ref80","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2018.2432"},{"key":"S0007485323000147_ref48","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0177643"},{"key":"S0007485323000147_ref64","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12180"},{"key":"S0007485323000147_ref36","doi-asserted-by":"crossref","first-page":"e2807","DOI":"10.7717\/peerj.2807","article-title":"Spatio-temporal monitoring of deep-sea communities using metabarcoding of sediment DNA and RNA","volume":"4","author":"Guardiola","year":"2016","journal-title":"PeerJ"},{"key":"S0007485323000147_ref74","doi-asserted-by":"publisher","DOI":"10.1016\/j.gecco.2015.11.002"},{"key":"S0007485323000147_ref31","doi-asserted-by":"publisher","DOI":"10.1111\/mec.13238"},{"key":"S0007485323000147_ref23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2012.05537.x"},{"key":"S0007485323000147_ref77","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/42.5.726"},{"key":"S0007485323000147_ref34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022746"},{"key":"S0007485323000147_ref71","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2584"},{"key":"S0007485323000147_ref69","first-page":"1","article-title":"Os mosquitos (Diptera: Culicidae) da Ilha de S\u00e3o Tom\u00e9","volume":"22","author":"Ribeiro","year":"1998","journal-title":"Garcia de Orta Serie de Zoologia"},{"key":"S0007485323000147_ref86","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1080\/02755947.2017.1306005","article-title":"Comparing efficiency of American Fisheries Society standard snorkeling techniques to environmental DNA sampling techniques","volume":"37","author":"Ulibarri","year":"2017","journal-title":"North American Journal of Fisheries Management"},{"key":"S0007485323000147_ref33","volume-title":"A Supplement to the Anophelinae of Africa South of the Sahara","author":"Gillies","year":"1987"},{"key":"S0007485323000147_ref78","first-page":"100080","article-title":"Infectious disease and economics: the case for considering multi-sectoral impacts","volume":"7","author":"Smith","year":"2019","journal-title":"One Health (Amsterdam, Netherlands)"},{"key":"S0007485323000147_ref26","first-page":"3047","article-title":"MultiQC: summarize analysis results for multiple tools and samples in a single report","volume":"32","author":"Ewels","year":"2016","journal-title":"Bioinformatics (Oxford, England)"},{"key":"S0007485323000147_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2020.104357"},{"key":"S0007485323000147_ref61","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.pt.2009.03.013","article-title":"The role of monitoring mosquito infection in the global programme to eliminate lymphatic filariasis","volume":"25","author":"Pedersen","year":"2009","journal-title":"Trends in Parasitology"},{"key":"S0007485323000147_ref79","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.biocon.2014.11.016","article-title":"Using environmental DNA methods to improve detectability in a hellbender (Cryptobranchus alleganiensis) monitoring program","volume":"183","author":"Spear","year":"2015","journal-title":"Biological Conservation"},{"key":"S0007485323000147_ref85","doi-asserted-by":"publisher","DOI":"10.1017\/S0266467408005658"},{"key":"S0007485323000147_ref92","first-page":"e03463","article-title":"Shifts in mosquito diversity and abundance along a gradient from oil palm plantations to conterminous forests in Borneo","volume":"12","author":"Young","year":"2021","journal-title":"Ecosphere (Washington, D.C.)"},{"key":"S0007485323000147_ref18","doi-asserted-by":"publisher","DOI":"10.1111\/mec.14350"},{"key":"S0007485323000147_ref53","first-page":"2957","article-title":"FLASH: fast length adjustment of short reads to improve genome assemblies","volume":"27","author":"Mago\u010d","year":"2011","journal-title":"Bioinformatics (Oxford, England)"},{"key":"S0007485323000147_ref89","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-016-2104-5"},{"key":"S0007485323000147_ref63","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.actatropica.2017.04.003","article-title":"Culex (Culiciomyia) sasai (Diptera: Culicidae), senior synonym of Cx. spiculothorax and a new country record for Bhutan","volume":"171","author":"Phanitchakun","year":"2017","journal-title":"Acta Tropica"},{"key":"S0007485323000147_ref82","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007137"},{"key":"S0007485323000147_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2016.02.008"},{"key":"S0007485323000147_ref54","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet.Journal"},{"key":"S0007485323000147_ref24","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaz4520"},{"key":"S0007485323000147_ref43","unstructured":"Illumina, I (2017) Effects of index misassignment on multiplexing and downstream analysis. Available at https:\/\/www.illumina.com."},{"key":"S0007485323000147_ref29","volume-title":"A Review of Entomological Sampling Methods and Indicators for Dengue Vectors","author":"Focks","year":"2004"},{"key":"S0007485323000147_ref15","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1146\/annurev-ento-120811-153628","article-title":"Molecular systematics and insecticide resistance in the major African malaria vector Anopheles funestus","volume":"58","author":"Coetzee","year":"2013","journal-title":"Annual Review in Entomology"},{"key":"S0007485323000147_ref35","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1111\/2041-210X.12595","article-title":"Critical considerations for the application of environmental DNA methods to detect aquatic species","volume":"7","author":"Goldberg","year":"2016","journal-title":"Methods in Ecology and Evolution"},{"key":"S0007485323000147_ref41","volume-title":"Invasive species in a changing world","author":"Harold","year":"2000"},{"key":"S0007485323000147_ref67","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2017.03.035"},{"key":"S0007485323000147_ref30","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/44.4.554"},{"key":"S0007485323000147_ref91","doi-asserted-by":"crossref","first-page":"e0009063","DOI":"10.1371\/journal.pntd.0009063","article-title":"Modelling distributions of Aedes aegypti and Aedes albopictus using climate, host density and interspecies competition","volume":"15","author":"Yang","year":"2021","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"S0007485323000147_ref22","doi-asserted-by":"publisher","DOI":"10.1139\/gen-2018-0071"},{"key":"S0007485323000147_ref32","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.aspen.2016.04.016","article-title":"Effects of monoculture and polyculture farming in oil palm smallholdings on terrestrial arthropod diversity","volume":"19","author":"Ghazali","year":"2016","journal-title":"Journal of Asia-Pacific Entomology"},{"key":"S0007485323000147_ref40","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2018.03.003"},{"key":"S0007485323000147_ref90","unstructured":"World Health Organization (2020) World malaria report 2020: 20 years of global progress and challenges."},{"key":"S0007485323000147_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.baae.2009.12.009"},{"key":"S0007485323000147_ref21","volume-title":"Mosquitoes of the Ethiopian Region. III.-Culicine Adults and Pupae","author":"Edwards","year":"1941"},{"key":"S0007485323000147_ref11","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13950"},{"key":"S0007485323000147_ref56","doi-asserted-by":"publisher","DOI":"10.1111\/jfb.12863"},{"key":"S0007485323000147_ref58","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.56"},{"key":"S0007485323000147_ref8","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-018-0470-z"}],"container-title":["Bulletin of Entomological Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007485323000147","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T07:00:42Z","timestamp":1692255642000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007485323000147\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,15]]},"references-count":93,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["S0007485323000147"],"URL":"https:\/\/doi.org\/10.1017\/s0007485323000147","relation":{},"ISSN":["0007-4853","1475-2670"],"issn-type":[{"value":"0007-4853","type":"print"},{"value":"1475-2670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,15]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2023. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}