{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T18:05:13Z","timestamp":1759601113354,"version":"3.40.5"},"reference-count":49,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T00:00:00Z","timestamp":1727395200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Bull. Entomol. Res."],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Drosophila suzukii<\/jats:italic> is a pest of global concern due to its great impact on several crops. The entomopathogenic nematode <jats:italic>Steinernema carpocapsae<\/jats:italic> was highly virulent to the larvae of the fly although some immune mechanisms were triggered along the infection course. Thus, to understand the gene activation profile we performed a comparative transcriptome of <jats:italic>D. suzukii<\/jats:italic> larvae infected with <jats:italic>S. carpocapsae<\/jats:italic> and <jats:italic>Xenorhabdus nematophila<\/jats:italic> to map the differentially expressed genes involved in the defence response. The analysis exposed the induction of genes involved in the humoral response such as the antimicrobial peptides and pattern-recognition receptors while there was a suppression of the cellular defence. Besides, genes involved in melanisation, and clot formation were downregulated hindering the encapsulation response and wound healing. After the infection, larvae were in a stress condition with an enrichment of metabolic and transport functionalities. Concerning the stress response, we observed variations of the heat-shock proteins, detoxification, and peroxidase enzymes. These findings set a genetical comprehensive knowledge of the host-pathogen relation of <jats:italic>D. suzukii<\/jats:italic> challenged with <jats:italic>S. carpocapsae<\/jats:italic> which could support further comparative studies with entomopathogenic nematodes.<\/jats:p>","DOI":"10.1017\/s0007485324000543","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T09:20:03Z","timestamp":1727428803000},"page":"622-630","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":2,"title":["Analysis of the immune transcriptome of the invasive pest spotted wing drosophila infected by <i>Steinernema carpocapsae<\/i>"],"prefix":"10.1017","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9198-652X","authenticated-orcid":false,"given":"A.","family":"Garriga","sequence":"first","affiliation":[]},{"given":"D.","family":"Toubarro","sequence":"additional","affiliation":[]},{"given":"A.","family":"Morton","sequence":"additional","affiliation":[]},{"given":"N.","family":"Sim\u00f5es","sequence":"additional","affiliation":[]},{"given":"F.","family":"Garc\u00eda-del-Pino","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2024,9,27]]},"reference":[{"volume-title":"R: A Language and Environment for Statistical Computing","year":"2017","key":"S0007485324000543_ref37"},{"key":"S0007485324000543_ref40","doi-asserted-by":"publisher","DOI":"10.1186\/s12898-016-0070-3"},{"key":"S0007485324000543_ref48","doi-asserted-by":"publisher","DOI":"10.1534\/g3.117.041004"},{"key":"S0007485324000543_ref44","first-page":"143","volume-title":"Drosophila suzukii Management","author":"Wang","year":"2021"},{"key":"S0007485324000543_ref43","doi-asserted-by":"publisher","DOI":"10.1603\/IPM10010"},{"key":"S0007485324000543_ref26","first-page":"1","article-title":"Immune-related genes of the larval Holotrichia parallela in response to entomopathogenic nematodes Heterorhabditis beicherriana LF","volume":"22","author":"Li","year":"2021","journal-title":"BMC Genomics"},{"key":"S0007485324000543_ref36","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02740-14"},{"key":"S0007485324000543_ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-015-0681-z"},{"key":"S0007485324000543_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2007.12.008"},{"key":"S0007485324000543_ref13","doi-asserted-by":"publisher","DOI":"10.3390\/insects11060353"},{"key":"S0007485324000543_ref29","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"S0007485324000543_ref20","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-18722-7"},{"key":"S0007485324000543_ref12","doi-asserted-by":"publisher","DOI":"10.3390\/insects11010062"},{"key":"S0007485324000543_ref33","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12498"},{"key":"S0007485324000543_ref16","first-page":"639","article-title":"Soil emergence of Drosophila suzukii adults: a susceptible period for entomopathogenic nematodes infection","volume":"93","author":"Garriga","year":"2020","journal-title":"Journal of Pesticide Science"},{"key":"S0007485324000543_ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2008.02.003"},{"key":"S0007485324000543_ref46","first-page":"100141","article-title":"Clusterprofiler 4.0: a universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innovation"},{"key":"S0007485324000543_ref49","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0192183"},{"key":"S0007485324000543_ref25","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-323"},{"key":"S0007485324000543_ref42","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075691"},{"volume-title":"Steinernematid and Heterorhabditid Nematodes: A Handbook of Biology and Techniques","year":"1988","author":"Woodring","key":"S0007485324000543_ref45"},{"key":"S0007485324000543_ref14","first-page":"789","article-title":"Is Drosophila suzukii as susceptible to entomopathogenic nematodes as Drosophila melanogaster?","volume":"91","author":"Garriga","year":"2018","journal-title":"Journal of Pesticide Science"},{"key":"S0007485324000543_ref19","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49410-8"},{"key":"S0007485324000543_ref7","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4905"},{"key":"S0007485324000543_ref3","unstructured":"Andrews, S (2010) FastQC: a quality control tool for high throughput sequence data [Online]. 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