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Mastitis represents one of most significant diseases of small ruminant production worldwide. <jats:italic>Staphylococcus aureus, Mannheimia haemolytica, Streptococcus uberis<\/jats:italic> and coagulase-negative Staphylococcal species are common pathogens isolated from cases of sheep mastitis. Sampling kits supplied to 23 farms provided 33 samples for bacteriology, antimicrobial susceptibility testing and genetic analysis. Of the bacterial isolates identified, 60% were <jats:italic>S. aureus<\/jats:italic>, 23% <jats:italic>M. haemolytica<\/jats:italic> and 7% coagulase-negative staphylococci. Low levels of antimicrobial resistance were identified in the <jats:italic>S. aureus<\/jats:italic> isolates which provided novel multi-locus sequence types. In conclusion, this proof-of-concept survey demonstrated that mammary secretions free from environmental contamination may be provided by sheep farmers. 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