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In this study we screened for the presence of parasitic nematodes infecting lacertid lizards from the Iberian Peninsula and Balearic islands using nematode-specific 18S ribosomal RNA gene primers on host tissue samples. Sequencing of positive samples revealed the presence of different genera of nematodes. The detection of <jats:italic>Strongyloides,<\/jats:italic> a monoxenous genus reported for the first time in <jats:italic>Podarcis lilfordi<\/jats:italic> is probably the result of the amplification of larval stages present in the host circulatory system. Two spirurid nematodes, <jats:italic>Synhimantus<\/jats:italic> and a new unidentified clade, were also found, suggesting that reptiles might be paratenic hosts of several spirurid species. This study demonstrates the benefits of using specific molecular markers on tissue samples to identify infecting larval stages of nematodes, otherwise difficult to assess using traditional screening methods.<\/jats:p>","DOI":"10.1017\/s0022149x12000181","type":"journal-article","created":{"date-parts":[[2012,4,2]],"date-time":"2012-04-02T09:32:19Z","timestamp":1333359139000},"page":"189-194","source":"Crossref","is-referenced-by-count":14,"title":["Molecular screening of nematodes in lacertid lizards from the Iberian Peninsula and Balearic Islands using 18S rRNA sequences"],"prefix":"10.1017","volume":"87","author":[{"given":"A.","family":"Perera","sequence":"first","affiliation":[]},{"given":"J.P.M.C.","family":"Maia","sequence":"additional","affiliation":[]},{"given":"F.","family":"Jorge","sequence":"additional","affiliation":[]},{"given":"D.J.","family":"Harris","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2012,4,2]]},"reference":[{"key":"S0022149X12000181_ref9","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X09990381"},{"key":"S0022149X12000181_ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-008-1269-9"},{"key":"S0022149X12000181_ref37","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"S0022149X12000181_ref27","doi-asserted-by":"publisher","DOI":"10.1007\/s00114-009-0564-3"},{"key":"S0022149X12000181_ref16","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2699.2002.00744.x"},{"key":"S0022149X12000181_ref31","first-page":"369","article-title":"Strongyloides ophiusensis sp. n (Nematoda: Strongyloididae), parasite of an insular lizard, Podarcis pityusensis (Sauria, Lacertidae)","volume":"39","author":"Roca","year":"1992","journal-title":"Folia Parasitologica"},{"key":"S0022149X12000181_ref26","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2666.1"},{"key":"S0022149X12000181_ref24","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-011-9311-1"},{"key":"S0022149X12000181_ref4","doi-asserted-by":"publisher","DOI":"10.1079\/9781845935726.0000"},{"key":"S0022149X12000181_ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2005.06.012"},{"key":"S0022149X12000181_ref35","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-003-1042-z"},{"key":"S0022149X12000181_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2009.10.020"},{"key":"S0022149X12000181_ref22","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-4-18"},{"key":"S0022149X12000181_ref12","doi-asserted-by":"publisher","DOI":"10.1079\/9780851994239.0053"},{"key":"S0022149X12000181_ref33","first-page":"705","volume-title":"Fauna Ib\u00e9rica: Reptiles, vol. 10","author":"Salvador","year":"1997"},{"key":"S0022149X12000181_ref2","doi-asserted-by":"publisher","DOI":"10.1139\/z84-050"},{"key":"S0022149X12000181_ref17","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2470.1"},{"key":"S0022149X12000181_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2008.10.013"},{"key":"S0022149X12000181_ref3","doi-asserted-by":"publisher","DOI":"10.1079\/9780851994215.0000"},{"key":"S0022149X12000181_ref6","doi-asserted-by":"publisher","DOI":"10.1079\/9781845935726.0334"},{"key":"S0022149X12000181_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(02)00156-X"},{"key":"S0022149X12000181_ref14","first-page":"240","article-title":"Nestling diet and fledgling production of Eurasian kestrels (Falco tinnunculus) in eastern Spain","volume":"29","author":"Gil-Delgado","year":"1995","journal-title":"Journal of Raptor Research"},{"key":"S0022149X12000181_ref10","unstructured":"Drummond A.J. , Ashton B. , Buxton S. , Cheung M. , Cooper A. , Duran C. , Field M. , Heled J. , Kearse M. , Markowitz S. , Moir R. , Stones-Havas S. , Sturrock S. , Thierer T. & Wilson A. 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