{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T17:12:02Z","timestamp":1751476322542,"version":"3.41.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2004,2,18]],"date-time":"2004-02-18T00:00:00Z","timestamp":1077062400000},"content-version":"unspecified","delay-in-days":17,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mar. Biol. Ass."],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:p>In this study randomly amplified polymorphic DNA (RAPD) fingerprinting is proposed for species identification of <jats:italic>Gammarus<\/jats:italic>, based on the electrophoretic position of amplified DNA bands. Three common marine amphipods of European estuaries\u2014<jats:italic>G. chevreuxi<\/jats:italic>, <jats:italic>G. insensibilis<\/jats:italic> and <jats:italic>G. locusta<\/jats:italic>\u2014were profiled using ten RAPD primers, accompanied by a careful morphological identification. Nine of these primers produced a very distinct species-specific RAPD profile, allowing unambiguous differentiation of amphipod species assayed. The RAPD fingerprints were here characterized by 8\u201312 amplicons for each primer. Each amplicon was visualized as a band of known molecular length, with characteristic band thickness and density. A total of 78 diagnostic bands, based on the most robust and evident amplicons found for each primer and species, are proposed for identification of the <jats:italic>Gammarus<\/jats:italic> species analysed. These results allowed us to identify an unknown amphipod species from a previous study as <jats:italic>G. insensibilis<\/jats:italic>, only based on the RAPD fingerprint. One primer was sufficient for this identification. A taxonomic identification system integrating molecular and morphological tools is proposed for <jats:italic>Gammarus<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0025315404009038h","type":"journal-article","created":{"date-parts":[[2004,2,20]],"date-time":"2004-02-20T09:02:47Z","timestamp":1077267767000},"page":"171-178","source":"Crossref","is-referenced-by-count":11,"title":["Application of RAPD DNA fingerprinting in taxonomic identification of amphipods: a case-study with <i>Gammarus<\/i> species (Crustacea: Amphipoda)"],"prefix":"10.1017","volume":"84","author":[{"given":"Filipe O.","family":"Costa","sequence":"first","affiliation":[]},{"given":"Marina R.","family":"Cunha","sequence":"additional","affiliation":[]},{"given":"Teresa","family":"Neuparth","sequence":"additional","affiliation":[]},{"given":"Christopher W.","family":"Theodorakis","sequence":"additional","affiliation":[]},{"given":"M. Helena","family":"Costa","sequence":"additional","affiliation":[]},{"given":"Lee R.","family":"Shugart","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2004,2,18]]},"container-title":["Journal of the Marine Biological Association of the United Kingdom"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0025315404009038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T22:24:53Z","timestamp":1750458293000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0025315404009038\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["S0025315404009038"],"URL":"https:\/\/doi.org\/10.1017\/s0025315404009038h","relation":{},"ISSN":["0025-3154","1469-7769"],"issn-type":[{"type":"print","value":"0025-3154"},{"type":"electronic","value":"1469-7769"}],"subject":[],"published":{"date-parts":[[2004,2]]}}}