{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T12:53:40Z","timestamp":1762520020816},"reference-count":13,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2004,3,3]],"date-time":"2004-03-03T00:00:00Z","timestamp":1078272000000},"content-version":"unspecified","delay-in-days":2,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:p>Intergenic region typing by restriction analysis of the ribosomal internal transcribed spacer (ITS) and mini-exon provide diagnostic markers for some<jats:italic>Leishmania<\/jats:italic>. Here, we evaluate restriction analysis of these targets for genotyping and phylogenetic analysis within the<jats:italic>Leishmania donovani<\/jats:italic>complex (agents of visceral leishmaniasis). Each method was useful for genotyping of both<jats:italic>L. donovani<\/jats:italic>complex strains and Old World<jats:italic>Leishmania<\/jats:italic>species. The targets produced less robust groups than gp63 intergenic regions, but support the need for re-evaluation of the taxonomy of the<jats:italic>L. donovani<\/jats:italic>complex.<\/jats:p>","DOI":"10.1017\/s0031182003004578","type":"journal-article","created":{"date-parts":[[2004,3,3]],"date-time":"2004-03-03T09:53:46Z","timestamp":1078307626000},"page":"263-267","source":"Crossref","is-referenced-by-count":61,"title":["<i>Leishmania donovani<\/i>complex: genotyping with the ribosomal internal transcribed spacer and the mini-exon"],"prefix":"10.1017","volume":"128","author":[{"given":"I. L.","family":"MAURICIO","sequence":"first","affiliation":[]},{"given":"J. R.","family":"STOTHARD","sequence":"additional","affiliation":[]},{"given":"M. A.","family":"MILES","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2004,3,3]]},"reference":[{"key":"S0031182003004578_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/0001-706X(95)00137-4"},{"key":"S0031182003004578_ref011","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/1990653111"},{"key":"S0031182003004578_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(94)90153-8"},{"key":"S0031182003004578_ref003","unstructured":"FELSENSTEIN, J. (1993). PHYLIP (Phylogeny Inference Package). Seattle, Distributed by the author. Department of Genetics, University of Washington."},{"key":"S0031182003004578_ref002","doi-asserted-by":"publisher","DOI":"10.1006\/expr.2001.4592"},{"key":"S0031182003004578_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(95)00108-D"},{"key":"S0031182003004578_ref007","doi-asserted-by":"crossref","unstructured":"KATAKURA, K. , KAWAZU, S. , NAYA, T. , NAGAKURA, K. , ITO, M. , AIKAWA, M. , QU, J. Q. , GUAN, L. R. , ZUO, X. P. , CHAI, J. J. , CHANG, K. P. & MATSUMOTO, Y. (1998).Diagnosis of kala-azar by nested PCR based on amplification of the Leishmania mini-exon gene.Journal of Clinical Microbiology 36, 2173\u20132177.","DOI":"10.1128\/JCM.36.8.2173-2177.1998"},{"key":"S0031182003004578_ref006","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000068086"},{"key":"S0031182003004578_ref008","doi-asserted-by":"publisher","DOI":"10.1385\/0-89603-239-6:123"},{"key":"S0031182003004578_ref010","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1996.0031"},{"key":"S0031182003004578_ref009","doi-asserted-by":"crossref","unstructured":"MAURICIO, I. L. , GAUNT, M. W. , STOTHARD, J. R. & MILES, M. A. (2001).Genetic typing and phylogenetic analysis of the Leishmania donovani complex by restriction analysis of PCR amplified gp63 intergenic regions.Parasitology 122, 393\u2013403.","DOI":"10.1017\/S0031182001007466"},{"key":"S0031182003004578_ref012","doi-asserted-by":"publisher","DOI":"10.1007\/s004300100081"},{"key":"S0031182003004578_ref005","doi-asserted-by":"crossref","unstructured":"HARRIS, E. , KROPP, G. , BELLI, A. , RODRIGUEZ, B. & AGABIAN, N. (1998).Single-step multiplex PCR assay for characterization of New World Leishmania complexes.Journal of Clinical Microbiology 36, 1989\u20131995.","DOI":"10.1128\/JCM.36.7.1989-1995.1998"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182003004578","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T09:40:11Z","timestamp":1585647611000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182003004578\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,3]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,3]]}},"alternative-id":["S0031182003004578"],"URL":"https:\/\/doi.org\/10.1017\/s0031182003004578","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,3]]}}}