{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T05:53:22Z","timestamp":1763704402957},"reference-count":44,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T00:00:00Z","timestamp":1172620800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Didymobothrium rudolphii<\/jats:italic>(Cestoda: Spathebothriidea) was collected seasonally from the sand sole,<jats:italic>Solea lascaris<\/jats:italic>, off the northern, central and southern areas of the Portuguese coast. Morphological and molecular analyses were conducted in order to examine the possible existence of cryptic species and to facilitate the circumscription of their morphological boundaries. Data were compared between<jats:italic>D. rudolphii<\/jats:italic>specimens from each of the 3 geographical areas and 4 seasons, and principal components analysis of 18 morphological characters was used to detect differences. Two distinct genotypes were present with sequence divergences of 1\u00b79% and 2\u00b71% in the large subunit (lsrDNA) and second internal transcribed spacer (ITS-2) of ribosomal DNA (rDNA), respectively. The less common \u2018central\u2019 genotype was present only off the central area from summer to winter, whereas the \u2018common\u2019 genotype was present throughout the year off the northern and southern areas, but only during spring in the central area. No sequence variation was found within each genotype. The presence of 2 distinct genetic entities was supported by morphological analyses, which showed the \u2018central\u2019 genotype specimens to be more slender and elongate, although morphometric ranges overlapped considerably for most characters. Molecular phylogenetic analysis of 4 of the 5 known genera of the Spathebothriidea showed<jats:italic>Spathebothrium<\/jats:italic>to be the earliest branching lineage and the 2 genotypes of<jats:italic>Didymobothrium<\/jats:italic>formed a sister group to<jats:italic>Cyathocephalus<\/jats:italic>. The concordance of genetic differences with variation in host diet according to season and locality could account for sympatric speciation occurring in the central region of the Portuguese coast.<\/jats:p>","DOI":"10.1017\/s0031182007002491","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T10:15:12Z","timestamp":1172657712000},"page":"1057-1072","source":"Crossref","is-referenced-by-count":17,"title":["Cryptic species of<i>Didymobothrium rudolphii<\/i>(Cestoda: Spathebothriidea) from the sand sole,<i>Solea lascaris<\/i>, off the Portuguese coast, with an analysis of their molecules, morphology, ultrastructure and phylogeny"],"prefix":"10.1017","volume":"134","author":[{"given":"J.\u00a0F.","family":"MARQUES","sequence":"first","affiliation":[]},{"given":"M.\u00a0J.","family":"SANTOS","sequence":"additional","affiliation":[]},{"given":"D.\u00a0I.","family":"GIBSON","sequence":"additional","affiliation":[]},{"given":"H.\u00a0N.","family":"CABRAL","sequence":"additional","affiliation":[]},{"given":"P.\u00a0D.","family":"OLSON","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2007,2,28]]},"reference":[{"key":"S0031182007002491_ref044","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006252601201"},{"key":"S0031182007002491_ref043","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a025802"},{"key":"S0031182007002491_ref040","volume-title":"PAUP*. 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Zoologica"},{"key":"S0031182007002491_ref008","first-page":"279","article-title":"In toto staining method for cestodes with iron acetocarmine","volume":"23","author":"Georgiev","year":"1986","journal-title":"Helminthologia"},{"key":"S0031182007002491_ref007","first-page":"132","article-title":"An ultrastructure of some systems of the Diplocotyle olrikii (Cestoda: Cyathocephalata) in relation to peculiarities of its life cycle","volume":"31","author":"Davydov","year":"1997","journal-title":"Parazitologiya"},{"key":"S0031182007002491_ref006","doi-asserted-by":"publisher","DOI":"10.3989\/scimar.2002.66n3293"},{"key":"S0031182007002491_ref005","doi-asserted-by":"publisher","DOI":"10.1139\/f69-095"},{"key":"S0031182007002491_ref004","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-005-1378-7"},{"key":"S0031182007002491_ref003","doi-asserted-by":"publisher","DOI":"10.1139\/z01-189"},{"key":"S0031182007002491_ref001","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182004006754"},{"key":"S0031182007002491_ref033","unstructured":"Protasova E.\u00a0N. and Roytman V.\u00a0A. (1995). [Cyathocephalates, tapeworm helminths of marine and freshwater fish (Cestoda: Pseudophyllidea: Cyathocephalata).] Osnovy Tsestodologii, Vol. 12. Institute of Parasitology, Russian Academy of Sciences, Moscow."},{"key":"S0031182007002491_ref017","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh435"},{"key":"S0031182007002491_ref015","doi-asserted-by":"publisher","DOI":"10.1139\/z84-152"},{"key":"S0031182007002491_ref024","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(03)00049-3"},{"key":"S0031182007002491_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2006.10.010"},{"key":"S0031182007002491_ref041","year":"2004"},{"key":"S0031182007002491_ref029","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2003.047"},{"key":"S0031182007002491_ref020","first-page":"102","article-title":"The use and implications of ribosomal DNA sequencing for the discrimination of digenean species","volume":"60","author":"Nolan","year":"2005","journal-title":"Advances in Parasitology"},{"key":"S0031182007002491_ref009","first-page":"15","volume-title":"Keys to the Cestode Parasites of Vertebrates","author":"Gibson","year":"1994"},{"key":"S0031182007002491_ref042","volume-title":"Canoco for Windows Version 4.5","author":"ter Braak","year":"2002"},{"key":"S0031182007002491_ref013","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1002\/9780470995259.ch9","volume-title":"Flatfishes Biology and Exploitation","author":"Link","year":"2005"},{"key":"S0031182007002491_ref019","first-page":"100","article-title":"Sul genere Bothrimonus, Duvernoy e proposte per una classificazione dei Cestodi","volume":"5","author":"Monticelli","year":"1892","journal-title":"Monitore Zoologico Italiano"},{"key":"S0031182007002491_ref034","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000066816"},{"key":"S0031182007002491_ref031","doi-asserted-by":"publisher","DOI":"10.2478\/s11686-006-0039-z"},{"key":"S0031182007002491_ref032","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/14.9.817"},{"key":"S0031182007002491_ref036","doi-asserted-by":"publisher","DOI":"10.1645\/GE-3143"},{"key":"S0031182007002491_ref028","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2002.012"},{"key":"S0031182007002491_ref012","doi-asserted-by":"publisher","DOI":"10.1007\/s10126-004-3081-6"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182007002491","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,11]],"date-time":"2024-02-11T08:10:21Z","timestamp":1707639021000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182007002491\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2,28]]},"references-count":44,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["S0031182007002491"],"URL":"https:\/\/doi.org\/10.1017\/s0031182007002491","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,2,28]]}}}