{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T08:08:38Z","timestamp":1771661318140,"version":"3.50.1"},"reference-count":17,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2010,3,17]],"date-time":"2010-03-17T00:00:00Z","timestamp":1268784000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>PCR assays have been developed mainly to assist investigations into the epidemiology of <jats:italic>Giardia duodenalis<\/jats:italic>, the only species in the <jats:italic>Giardia<\/jats:italic> genus having zoonotic potential. However, a reliable identification of all species is of practical importance, particularly when water samples and samples from wild animals are investigated. The aim of the present work was to genotype <jats:italic>Giardia<\/jats:italic> species and <jats:italic>G. duodenalis<\/jats:italic> assemblages using as a target the region spanning the 5.8S gene and the 2 flanking internal transcribed spacers (ITS1 and ITS2) of the ribosomal gene. Primers were designed to match strongly conserved regions in the 3\u2032 end of the small subunit and in the 5\u2032 end of the large subunit ribosomal genes. The corresponding region (about 310 bp) was amplified from 49 isolates of both human and animal origin, representing all <jats:italic>G. duodenalis<\/jats:italic> assemblages as well as <jats:italic>G. muris<\/jats:italic> and <jats:italic>G. microti<\/jats:italic>. Sequence comparison and phylogenetic analysis showed that <jats:italic>G. ardeae, G. muris<\/jats:italic>, <jats:italic>G. microti<\/jats:italic> as well as the 7 <jats:italic>G. duodenalis<\/jats:italic> assemblages can be easily distinguished. Since the major subgroups within the zoonotic assemblages A and B can be identified by sequence analysis, this assay is also informative for molecular epidemiological studies.<\/jats:p>","DOI":"10.1017\/s003118200999179x","type":"journal-article","created":{"date-parts":[[2010,3,17]],"date-time":"2010-03-17T10:02:52Z","timestamp":1268820172000},"page":"919-925","source":"Crossref","is-referenced-by-count":33,"title":["Identification of <i>Giardia<\/i> species and <i>Giardia duodenalis<\/i> assemblages by sequence analysis of the 5.8S rDNA gene and internal transcribed spacers"],"prefix":"10.1017","volume":"137","author":[{"given":"SIMONE M.","family":"CACCI\u00d2","sequence":"first","affiliation":[]},{"given":"RELJA","family":"BECK","sequence":"additional","affiliation":[]},{"given":"ANDRE","family":"ALMEIDA","sequence":"additional","affiliation":[]},{"given":"ANNA","family":"BAJER","sequence":"additional","affiliation":[]},{"given":"EDOARDO","family":"POZIO","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2010,3,17]]},"reference":[{"key":"S003118200999179X_ref005","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.19.7889"},{"key":"S003118200999179X_ref001","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.447-475.2001"},{"key":"S003118200999179X_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2008.04.006"},{"key":"S003118200999179X_ref006","doi-asserted-by":"publisher","DOI":"10.2307\/3284315"},{"key":"S003118200999179X_ref014","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.21.6050"},{"key":"S003118200999179X_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.09.008"},{"key":"S003118200999179X_ref012","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.24.4876"},{"key":"S003118200999179X_ref002","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.10.4065"},{"key":"S003118200999179X_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00259-4"},{"key":"S003118200999179X_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-1348(02)00149-1"},{"key":"S003118200999179X_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2008.11.006"},{"key":"S003118200999179X_ref010","doi-asserted-by":"publisher","DOI":"10.3201\/eid0911.030084"},{"key":"S003118200999179X_ref011","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"S003118200999179X_ref015","doi-asserted-by":"publisher","DOI":"10.1007\/BF00161169"},{"key":"S003118200999179X_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(02)00181-4"},{"key":"S003118200999179X_ref017","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182007003071"},{"key":"S003118200999179X_ref004","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm233"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118200999179X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,28]],"date-time":"2019-04-28T20:01:11Z","timestamp":1556481671000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118200999179X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,17]]},"references-count":17,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["S003118200999179X"],"URL":"https:\/\/doi.org\/10.1017\/s003118200999179x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,17]]}}}