{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:38:57Z","timestamp":1777455537599,"version":"3.51.4"},"reference-count":43,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2013,6,4]],"date-time":"2013-06-04T00:00:00Z","timestamp":1370304000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Many protease genes have previously been shown to be involved in parasitism and in the development of <jats:italic>Steinernema carpocapsae<\/jats:italic>, including a gene predicted to encode an aspartic protease, Sc-ASP110, which was cloned and was analysed in this study. A cDNA encoding Sc-ASP110 was cloned based on an expressed sequence tag (EST) fragment from our EST library. The full-length cDNA of Sc-ASP110 consists of 1112 nucleotides with a catalytic aspartic domain (aa18\u2013337). The putative 341 amino acid residues have a calculated molecular mass of 37\u00b71 kDa and a theoretical pI of 4\u00b77. BLASTp analysis of the Sc-ASP110 amino acid sequence showed 45\u201377% amino acid sequence identity to parasitic and non-parasitic nematode aspartic proteases. An expression analysis showed that the sc-asp110 gene was upregulated during the late parasitic stage, L4, and 24 h after induction of <jats:italic>in vitro<\/jats:italic> nematodes. A sequence comparison revealed that Sc-ASP110 was a member of an aspartic protease family; additionally, a phylogenetic analysis indicated that Sc-ASP110 was clustered with the closely related nematode <jats:italic>Steinernema feltiae. In situ<\/jats:italic> hybridization showed that sc-asp110 was expressed in the body walls of dorsal cells. The upregulated Sc-ASP110 expression revealed that this protease could play a role in the late parasitic process. In this study, we have cloned and analysed the gene transcript of Sc-ASP110 in <jats:italic>S. carpocapsae<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0031182013000577","type":"journal-article","created":{"date-parts":[[2013,6,4]],"date-time":"2013-06-04T08:46:30Z","timestamp":1370335590000},"page":"1158-1167","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":4,"title":["Cloning and molecular analysis of the aspartic protease Sc-ASP110 gene transcript in <i>Steinernema carpocapsae<\/i>"],"prefix":"10.1017","volume":"140","author":[{"given":"NATESAN","family":"BALASUBRAMANIAN","sequence":"first","affiliation":[]},{"given":"NELSON","family":"SIM\u00d5ES","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2013,6,4]]},"reference":[{"key":"S0031182013000577_ref28","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"S0031182013000577_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.11.008"},{"key":"S0031182013000577_ref37","first-page":"5","article-title":"The aspartic proteases. Scandinavian Journal of Clinical and Laboratory Investigation","volume":"210","author":"Szecsi","year":"1992","journal-title":"Supplementum"},{"key":"S0031182013000577_ref39","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.129346"},{"key":"S0031182013000577_ref26","first-page":"135","article-title":"Toxins of entomoparasitic nematodes. Pathogenicity of Steinernema carpocapsae prospectives of genetic engineering","volume":"75","author":"Laumond","year":"1989","journal-title":"Comptes rendus des s\u00e9ances de l'Acad\u00e9mie d'Agriculture de France"},{"key":"S0031182013000577_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2011(02)00038-1"},{"key":"S0031182013000577_ref2","volume-title":"Current Protocols in Molecular Biology","author":"Ausbel","year":"1989"},{"key":"S0031182013000577_ref33","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03577.x"},{"key":"S0031182013000577_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(00)00351-0"},{"key":"S0031182013000577_ref10","doi-asserted-by":"publisher","DOI":"10.1163\/187529282X00510"},{"key":"S0031182013000577_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2011.12.001"},{"key":"S0031182013000577_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.01.012"},{"key":"S0031182013000577_ref11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M213065200"},{"key":"S0031182013000577_ref12","first-page":"309","article-title":"In-situ hybridization to messenger RNA in Heterodera glycines","volume":"30","author":"de Boer","year":"1998","journal-title":"Journal of Nematology"},{"key":"S0031182013000577_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2009.10.002"},{"key":"S0031182013000577_ref16","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.51.1.47"},{"key":"S0031182013000577_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2012.03.062"},{"key":"S0031182013000577_ref25","doi-asserted-by":"publisher","DOI":"10.1007\/978-4-431-75655-2_10"},{"key":"S0031182013000577_ref20","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.py.27.090189.001011"},{"key":"S0031182013000577_ref30","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(90)90094-S"},{"key":"S0031182013000577_ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2009.01172.x"},{"key":"S0031182013000577_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2011.12.001"},{"key":"S0031182013000577_ref7","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.41.24496"},{"key":"S0031182013000577_ref8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000192"},{"key":"S0031182013000577_ref13","doi-asserted-by":"publisher","DOI":"10.1021\/cr010167q"},{"key":"S0031182013000577_ref17","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00679.x"},{"key":"S0031182013000577_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2164(08)70479-9"},{"key":"S0031182013000577_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(78)80613-9"},{"key":"S0031182013000577_ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s002530100711"},{"key":"S0031182013000577_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2010.07.004"},{"key":"S0031182013000577_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(97)00141-6"},{"key":"S0031182013000577_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2005.11.004"},{"key":"S0031182013000577_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2011(03)00101-0"},{"key":"S0031182013000577_ref38","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000956200"},{"key":"S0031182013000577_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.10.013"},{"key":"S0031182013000577_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(97)00074-1"},{"key":"S0031182013000577_ref43","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-009-1332-1"},{"key":"S0031182013000577_ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2004.05.017"},{"key":"S0031182013000577_ref32","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301639200"},{"key":"S0031182013000577_ref35","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbaexp.2004.11.004"},{"key":"S0031182013000577_ref40","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059878"},{"key":"S0031182013000577_ref41","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0181fje"},{"key":"S0031182013000577_ref42","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(03)00189-2"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182013000577","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T20:35:19Z","timestamp":1556051719000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182013000577\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,4]]},"references-count":43,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["S0031182013000577"],"URL":"https:\/\/doi.org\/10.1017\/s0031182013000577","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,4]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2013\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}