{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T06:56:47Z","timestamp":1773730607097,"version":"3.50.1"},"reference-count":39,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T00:00:00Z","timestamp":1495497600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Canine leishmaniosis (CanL) is a major veterinary concern and a public health issue. Serological data are essential for disease management. Several antigens used in serological assays have specificity related problems preventing relevant seropositivity values establishment. Herein we report significant seropositivity level disparity in a study cohort with 384 dogs from eight countries, for antigens traditionally used in CanL \u2013 soluble promastigote<jats:italic>Leishmania<\/jats:italic>antigens (SPLA) and K39 recombinant protein (rK39): 43\u00b78 and 2\u00b79% for SPLA and rK39, respectively. To better understand the reasons for this disparity, CanL-associated serological response was characterized using, for complement serological evaluation, a ubiquitous antigen \u2013 soluble<jats:italic>Escherichia coli<\/jats:italic>antigens (SECAs). Using cohorts of CanL dogs and dogs without clinical evidences of CanL from non-endemic regions of Portugal, the serological response of CanL animals followed specific trend of seropositivity rK39 &gt; SPLA &gt; SECA absent in non-diseased animals. Using receiver operating characteristic curve analysis, these characteristic trends were converted in ratios, SPLA\/SECA, rK39\/SECA and rK39\/SPLA, that presented high predictive for discriminating the CanL cohort that was potentiated when applied in a scoring system involving positivity to four out of five predictors (rK39, SPLA, SPLA\/SECA, rK39\/SECA and rK39\/SPLA). In fact, this approach discriminated CanL with similar sensitivity\/specificity as reference antigens, diminishing seropositivity in European cohort to 1\u00b78%. Ultimately, non-related antigens like SECA and seropositivity ratios between antigens enable different perspectives into serological data focusing on the search of characteristic serological signatures and not simple absolute serology values contributing to comprehensive serological status characterization.<\/jats:p>","DOI":"10.1017\/s0031182017000713","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T10:26:50Z","timestamp":1495535210000},"page":"1384-1393","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":8,"title":["The use of<i>Escherichia coli<\/i>total antigens as a complementary approach to address seropositivity to<i>Leishmania<\/i>antigens in canine leishmaniosis"],"prefix":"10.1017","volume":"144","author":[{"given":"CARLA","family":"LIMA","sequence":"first","affiliation":[]},{"given":"JO\u00c3O 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\u00a9 Cambridge University Press 2017\u00a0This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}