{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T17:59:45Z","timestamp":1774547985043,"version":"3.50.1"},"reference-count":41,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/0443\/2020; UIDP\/04423\/2020; UIDP\/50017\/2020;"],"award-info":[{"award-number":["UIDB\/0443\/2020; UIDP\/04423\/2020; UIDP\/50017\/2020;"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of <jats:italic>Perkinsus olseni<\/jats:italic> infection on the reproduction ability of clams has been underestimated so far. Although some studies found evidence of reduction of egg production and delay in gonad maturation after infection, the total effect of the infection is still unclear. In this study, <jats:italic>Ruditapes decussatus<\/jats:italic> clams from a na\u00efve population were injected with two different doses of <jats:italic>P. olseni<\/jats:italic> parasites, a low dose leading to a light infection and a high dose leading to a heavy infection. Clams were maintained during 2 months for maturation, and at the end of the experiment, the spawning was induced, the number of larvae release and mortality were evaluated. During the maturation period, infection level, gonadal stage, condition index, gross biochemical composition and oxidative status of progenitors were evaluated at days 0, 30 and 60 post-injection. The effects of <jats:italic>P. olseni<\/jats:italic> infection on clams showed alterations on biochemical parameters, namely lipid peroxidation, a significant mortality and a delayed gonad maturation, with a greater effect in the highly infected individuals. The reproductive capacity of the clams was impaired in both infected groups showing a lower production and a higher mortality rate of larvae. Finally, this study indicates that the production of natural beds with a high prevalence of <jats:italic>P. olseni<\/jats:italic> could be compromised by a deregulation of the natural reproduction cycle and a decrease in larvae production by infected animals, probably due to a combination of lower egg production and lower lipid reserves in larvae from infected clams.<\/jats:p>","DOI":"10.1017\/s0031182023000082","type":"journal-article","created":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T09:40:34Z","timestamp":1675244434000},"page":"321-328","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":4,"title":["Effect of the alveolate parasite <i>Perkinsus olseni<\/i> infection on sexual maturation and spawning efficiency of the clam <i>Ruditapes decussatus<\/i>"],"prefix":"10.1017","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2122-5973","authenticated-orcid":false,"given":"Sergio","family":"Fern\u00e1ndez-Boo","sequence":"first","affiliation":[]},{"given":"Catarina","family":"Duarte","sequence":"additional","affiliation":[]},{"given":"Eric","family":"Gu\u00e9v\u00e9lou","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Rosa","family":"Freitas","sequence":"additional","affiliation":[]},{"given":"Sandra","family":"Joaquim","sequence":"additional","affiliation":[]},{"given":"Benjamin","family":"Costas","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9862-9653","authenticated-orcid":false,"given":"Luisa","family":"Magalh\u00e3es","sequence":"additional","affiliation":[]},{"given":"Domit\u00edlia","family":"Matias","sequence":"additional","affiliation":[]},{"given":"Andreia","family":"Cruz","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2023,2,1]]},"reference":[{"key":"S0031182023000082_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2008.05.018"},{"key":"S0031182023000082_ref9","first-page":"1025","article-title":"Effects of Perkinsus marinus on reproduction and condition of the eastern oyster, Crassostrea virginica, depend on timing","volume":"20","author":"Dittman","year":"2001","journal-title":"Journal of Shellfish Research"},{"key":"S0031182023000082_ref5","first-page":"269","volume-title":"Coastal Environmental and Ecosystem Issues of the East China Sea","author":"Choi","year":"2010"},{"key":"S0031182023000082_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2018.04.432"},{"key":"S0031182023000082_ref29","first-page":"43","article-title":"Massive mortality in Manila clams (Ruditapes philippinarum) farmed in the lagoon of Venice, caused by Perkinsus olseni","volume":"34","author":"Pretto","year":"2014","journal-title":"Bulletin European Association of Fish Pathology"},{"key":"S0031182023000082_ref31","doi-asserted-by":"publisher","DOI":"10.1126\/science.116.3014.360"},{"key":"S0031182023000082_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2020.107479"},{"key":"S0031182023000082_ref36","doi-asserted-by":"publisher","DOI":"10.1021\/ac60032a019"},{"key":"S0031182023000082_ref25","doi-asserted-by":"publisher","DOI":"10.1007\/s10152-010-0230-3"},{"key":"S0031182023000082_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2004.05.014"},{"key":"S0031182023000082_ref3","unstructured":"Casas, SM (2002) Estudio de la perkinsosis en la almeja fina, Tapes decussatus (Linnaeus 1758), de Galicia (Ph.D. thesis). University of Santiago de Compostela, Spain."},{"key":"S0031182023000082_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2008.10.055"},{"key":"S0031182023000082_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"S0031182023000082_ref37","doi-asserted-by":"publisher","DOI":"10.1051\/alr:2004050"},{"key":"S0031182023000082_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2005.06.003"},{"key":"S0031182023000082_ref11","doi-asserted-by":"publisher","DOI":"10.3354\/dao004121"},{"key":"S0031182023000082_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2013.04.030"},{"key":"S0031182023000082_ref13","unstructured":"Heenan, S (2020) Aspects of reproduction in a post-Bonamia exitiosa epidemic Ostrea chilensis fishery, Foveaux Strait, Southern New Zealand. 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Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial licence (http:\/\/creativecommons.org\/licenses\/by-nc\/4.0), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original article is properly cited. The written permission of Cambridge University Press must be obtained prior to any commercial use.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}