{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:30:56Z","timestamp":1757701856751},"reference-count":23,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2011,7,29]],"date-time":"2011-07-29T00:00:00Z","timestamp":1311897600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Toscana virus (TOSV) is an emerging<jats:italic>Phlebovirus<\/jats:italic>of growing interest as a human pathogen in the Mediterranean Basin. In Portugal, however, little is known about the prevalence of TOSV infection. The aim of this work was to perform a seroprevalence study in patients with requests for laboratory diagnosis of vector-borne viruses. A total of 538 patients with and without neurological signs from 2004 to 2008 were studied by in-house indirect immunofluorescence assay and commercial enzyme-linked immunosorbent assays. A prevalence of 4\u00b72% for IgG antibodies was found in the group of patients with neurological signs. Five (3%) of these had recent infections. In the group with no neurological signs, the IgG prevalence was 1\u00b73%. Two samples, belonging to two patients, were also confirmed with plaque reduction neutralization tests with the TOSV ISS. Phl.3 Italian strain. This work showed that TOSV is present and causing disease from north to south in Portugal. The probable circulation of different phlebovirus serotypes in Portugal emphasizes the need for further studies.<\/jats:p>","DOI":"10.1017\/s0950268811001403","type":"journal-article","created":{"date-parts":[[2011,7,29]],"date-time":"2011-07-29T09:21:28Z","timestamp":1311931288000},"page":"1147-1150","source":"Crossref","is-referenced-by-count":16,"title":["Serological evidence of Toscana virus infection in Portuguese patients"],"prefix":"10.1017","volume":"140","author":[{"given":"F.","family":"AMARO","sequence":"first","affiliation":[]},{"given":"T.","family":"LUZ","sequence":"additional","affiliation":[]},{"given":"P.","family":"PARREIRA","sequence":"additional","affiliation":[]},{"given":"A.","family":"MARCHI","sequence":"additional","affiliation":[]},{"given":"M. G.","family":"CIUFOLINI","sequence":"additional","affiliation":[]},{"given":"M. J.","family":"ALVES","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2011,7,29]]},"reference":[{"key":"S0950268811001403_ref011","first-page":"41","article-title":"Sandfly fever viruses in Italy","volume":"11","author":"Nicoletti","year":"1996","journal-title":"Archives of Virology"},{"key":"S0950268811001403_ref020","doi-asserted-by":"publisher","DOI":"10.3201\/eid1303.061086"},{"key":"S0950268811001403_ref008","first-page":"57","article-title":"Travel-related vector-borne virus infections in Germany","volume":"11","author":"Schwarz","year":"1996","journal-title":"Archives of Virology"},{"key":"S0950268811001403_ref016","doi-asserted-by":"publisher","DOI":"10.1086\/319759"},{"key":"S0950268811001403_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(02)00041-0"},{"key":"S0950268811001403_ref002","first-page":"649","article-title":"Il virus Toscana: un nuovo Phlebovirus causa di malattie infiammatorie acute del SNC nell'uomo","volume":"38","author":"Leoncini","year":"1986","journal-title":"Giornale di malattie infettive e parassitarie"},{"key":"S0950268811001403_ref017","doi-asserted-by":"publisher","DOI":"10.1086\/381201"},{"key":"S0950268811001403_ref005","doi-asserted-by":"publisher","DOI":"10.3201\/eid1111.050869"},{"key":"S0950268811001403_ref018","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2008.01.016"},{"key":"S0950268811001403_ref021","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2008.0131"},{"key":"S0950268811001403_ref012","unstructured":"12. Pires C .Os fleb\u00f3tomos (Diptera, Psychodidae) dos focos zoon\u00f3ticos de leishmanioses em Portugal (Dissertation). Lisboa: Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, 2000, pp. 228."},{"key":"S0950268811001403_ref014","doi-asserted-by":"publisher","DOI":"10.3201\/eid1502.081001"},{"key":"S0950268811001403_ref001","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1988.38.433"},{"key":"S0950268811001403_ref009","doi-asserted-by":"crossref","unstructured":"9. Santos , Toscana virus meningitis in Portugal, 2002\u20132005. 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