{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:03:44Z","timestamp":1758816224543},"reference-count":71,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2014,9,26]],"date-time":"2014-09-26T00:00:00Z","timestamp":1411689600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The ability of early bovine embryos to produce prostaglandins (PGs) and progesterone (P<jats:sub>4<\/jats:sub>), and the role of these mediators in embryonic development and survival are poorly understood. In this study we tested the hypothesis that day 7 bovine embryos are able to transcribe genes coding for enzymes of the PGs (<jats:italic>PTGS2, PGES, PGFS<\/jats:italic>) and P<jats:sub>4<\/jats:sub> (<jats:italic>StAR, P450scc, 3\u03b2-HS<\/jats:italic>D) synthesis pathways, and that transcription levels of these genes are associated with developmental progression and heifer age-related [pre-pubertal (PP) versus post-pubertal cyclic (C)] oocyte competence. Compared with C heifer oocytes, PP heifer oocytes showed a lower (<jats:italic>P<\/jats:italic> &lt; 0.0001) <jats:italic>in vitro<\/jats:italic> blastocyst rate, but in embryos developing until day 7, heifer age had no effect on quality grade. Day 7 quality grade 1\u20132 embryos were selected for RNA extraction and gene transcription analysis by qRT-PCR, in a 2 \u00d7 2 factorial design [age (PP or C) \u00d7 embryonic stage (compact morulae and early blastocysts, CM + EBL, or blastocysts and expanded blastocysts, BL + BEX); 15 embryos\/group]. Transcription levels of <jats:italic>PTGS2, PGES, PGFS, P450scc<\/jats:italic> and <jats:italic>3\u03b2-HSD<\/jats:italic> were not affected by heifer age but were higher (<jats:italic>P<\/jats:italic> &lt; 0.01) in BL + BEX than in CM + EBL. In conclusion, the main limiting factor for embryo production from PP heifers is oocyte competence. Day 7 bovine embryos evidence transcription of genes coding for enzymes of PGs and P<jats:sub>4<\/jats:sub> synthesis pathways, and transcription levels are associated with blastocyst differentiation. This prompts for an autocrine\/paracrine action of PGs and P<jats:sub>4<\/jats:sub> in early bovine embryonic development.<\/jats:p>","DOI":"10.1017\/s0967199414000446","type":"journal-article","created":{"date-parts":[[2014,9,26]],"date-time":"2014-09-26T11:04:12Z","timestamp":1411729452000},"page":"802-812","source":"Crossref","is-referenced-by-count":8,"title":["Effects of oocyte donor age and embryonic stage of development on transcription of genes coding for enzymes of the prostaglandins and progesterone synthesis pathways in bovine <i>in vitro<\/i> produced embryos"],"prefix":"10.1017","volume":"23","author":[{"given":"A.","family":"Torres","sequence":"first","affiliation":[]},{"given":"M.","family":"Batista","sequence":"additional","affiliation":[]},{"given":"P.","family":"Diniz","sequence":"additional","affiliation":[]},{"given":"E.","family":"Silva","sequence":"additional","affiliation":[]},{"given":"L.","family":"Mateus","sequence":"additional","affiliation":[]},{"given":"L.","family":"Lopes-da-Costa","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2014,9,26]]},"reference":[{"key":"S0967199414000446_ref045","doi-asserted-by":"publisher","DOI":"10.1071\/R97047"},{"key":"S0967199414000446_ref050","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod56.2.386"},{"key":"S0967199414000446_ref071","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2005.12.1047"},{"key":"S0967199414000446_ref057","doi-asserted-by":"publisher","DOI":"10.1016\/j.prostaglandins.2004.02.001"},{"key":"S0967199414000446_ref055","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2005.10.015"},{"key":"S0967199414000446_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/S1642-431X(12)60012-6"},{"key":"S0967199414000446_ref030","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199610)45:2<231::AID-MRD17>3.0.CO;2-3"},{"key":"S0967199414000446_ref061","volume-title":"Manual of the International Embryo Transfer Society","author":"Stringfellow","year":"1998"},{"key":"S0967199414000446_ref028","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.09.010"},{"key":"S0967199414000446_ref070","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(97)82290-X"},{"key":"S0967199414000446_ref046","doi-asserted-by":"publisher","DOI":"10.1530\/REP-10-0113"},{"key":"S0967199414000446_ref011","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.5.1032"},{"key":"S0967199414000446_ref009","first-page":"188","article-title":"Embryonic development of the rat associated with elevated prostaglandin F2\u03b1","volume":"56","author":"Buuck","year":"1997","journal-title":"Biol. 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