{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T20:25:53Z","timestamp":1772742353696,"version":"3.50.1"},"reference-count":34,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The ultrastructural analysis of human oocytes at different maturation stages has only been descriptive. The aim of this study was to use a stereological approach to quantify the distribution of organelles in oocytes at prophase I (GV). Seven immature GV oocytes were processed for transmission electron microscopy and a classical manual stereological technique based on point-counting with an adequate stereological grid was used. The Kruskal\u2013Wallis test and Mann\u2013Whitney<jats:italic>U<\/jats:italic>-test with Bonferroni correction were used to compare the means of the relative volumes occupied by organelles in oocyte regions: cortex (C), subcortex (SC) and inner cytoplasm (IC). Here we first describe in GV oocytes very large vesicles of the smooth endoplasmic reticulum (SER), vesicles containing zona pellucida-like materials and coated vesicles. The most abundant organelles were the very large vesicles of the SER (6.9%), mitochondria (6.3%) and other SER vesicles (6.1%). Significant differences in organelle distribution were observed between ooplasm regions: cortical vesicles (C: 1.3% versus SC: 0.1%, IC: 0.1%,<jats:italic>P<\/jats:italic>= 0.001) and medium-sized vesicles containing zona pellucida-like materials (C: 0.2% versus SC: 0.02%, IC: 0%,<jats:italic>P<\/jats:italic>= 0.004) were mostly observed at the oocyte cortex, whereas mitochondria (C: 3.6% versus SC: 6.0%, IC: 7.2%,<jats:italic>P<\/jats:italic>= 0.005) were preferentially located in the subcortex and inner cytoplasm, and SER very large vesicles (IC: 10.1% versus C: 0.9%, SC: 1.67%,<jats:italic>P<\/jats:italic>= 0.001) in the oocyte inner cytoplasm. Further quantitative studies are needed in immature metaphase-I and mature metaphase-II oocytes, as well as analysis of correlations between ultrastructural and molecular data, to better understand human oocyte<jats:italic>in vitro<\/jats:italic>maturation.<\/jats:p>","DOI":"10.1017\/s0967199415000258","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T07:22:32Z","timestamp":1436858552000},"page":"346-354","source":"Crossref","is-referenced-by-count":12,"title":["A stereological study on organelle distribution in human oocytes at prophase I"],"prefix":"10.1017","volume":"24","author":[{"given":"Ana S\u00edlvia","family":"Pires-Lu\u00eds","sequence":"first","affiliation":[]},{"given":"Eduardo","family":"Rocha","sequence":"additional","affiliation":[]},{"given":"Carla","family":"Bartosch","sequence":"additional","affiliation":[]},{"given":"Elsa","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Joaquina","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Alberto","family":"Barros","sequence":"additional","affiliation":[]},{"given":"Ros\u00e1lia","family":"S\u00e1","sequence":"additional","affiliation":[]},{"given":"M\u00e1rio","family":"Sousa","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"S0967199415000258_ref034","doi-asserted-by":"publisher","DOI":"10.1093\/biolreprod\/7.3.425"},{"key":"S0967199415000258_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.04.088"},{"key":"S0967199415000258_ref015","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.21326"},{"key":"S0967199415000258_ref002","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/4.2.103"},{"key":"S0967199415000258_ref033","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.30.1.23"},{"key":"S0967199415000258_ref025","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2012.11.006"},{"key":"S0967199415000258_ref014","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2013.10.016"},{"key":"S0967199415000258_ref005","volume-title":"A Guide For Assisted Reproduction","author":"El Shafie","year":"2000"},{"key":"S0967199415000258_ref016","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00793"},{"key":"S0967199415000258_ref003","doi-asserted-by":"publisher","DOI":"10.1071\/RD06128"},{"key":"S0967199415000258_ref011","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gan064"},{"key":"S0967199415000258_ref010","first-page":"1","article-title":"Contribution of human oocyte architecture to success of in vitro maturation technology","volume":"11","author":"Khalili","year":"2013","journal-title":"Iran J. 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