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The nuclear maturation rates (NMR) of all oocytes were recorded after 24 h. In assay 2, oocytes collected year-round maturated, were implanted via <jats:italic>in vitro<\/jats:italic> fertilization (IVF) and developed for 9 days. Gene expression analysis was performed on target genes (<jats:italic>Cx43, CDH1, DNMT1, HSPA14<\/jats:italic>) with reference to the two housekeeping genes (<jats:italic>GAPDH<\/jats:italic> and <jats:italic>SDHA<\/jats:italic>) in embryos. Similarly, in assay 3, genetic analysis was performed on the embryos produced from heat-stressed oocytes (from HS1 and HS2). In assay 1, the duration of heat stress resulted in a significant decline in NMR (<jats:italic>P<\/jats:italic> &lt; 0.05) with HS1 for maturated oocytes at 86.4 \u00b1 4.3; 65.5 \u00b1 0.7; 51.3 \u00b1 0.9; 38.1 \u00b1 1.9 and 36.3 \u00b1 0.9, for control, 6 h, 12 h, 18 h and 24 h, respectively. For assays 2 and 3, results demonstrated that DNMT1, Cx43 and HSPA14 were down-regulated in the embryos produced in the warm with respect to the cold months (<jats:italic>P<\/jats:italic> &lt; 0.05). A constant up- and down-regulation of <jats:italic>DNMT1<\/jats:italic> and <jats:italic>HSPA14<\/jats:italic> genes were observed in both HS-treated samples. Also, an inconsistent pattern of gene expression was observed in <jats:italic>Cx43<\/jats:italic> and <jats:italic>CDH1<\/jats:italic> genes (<jats:italic>P<\/jats:italic> &lt; 0.05). Targeted gene expression was aberrant in embryo development, which can provide evidence on early embryo arrest and slowed embryo development.<\/jats:p>","DOI":"10.1017\/s0967199416000071","type":"journal-article","created":{"date-parts":[[2016,3,28]],"date-time":"2016-03-28T08:39:09Z","timestamp":1459154349000},"page":"748-759","source":"Crossref","is-referenced-by-count":32,"title":["Gene expression, oocyte nuclear maturation and developmental competence of bovine oocytes and embryos produced after <i>in vivo<\/i> and <i>in vitro<\/i> heat shock"],"prefix":"10.1017","volume":"24","author":[{"given":"Krishna C.","family":"Pavani","sequence":"first","affiliation":[]},{"given":"Erica","family":"Baron","sequence":"additional","affiliation":[]},{"given":"Pedro","family":"Correia","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Louren\u00e7o","sequence":"additional","affiliation":[]},{"given":"Bruno Filipe","family":"Bettencourt","sequence":"additional","affiliation":[]},{"given":"Madalena","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Fernando Moreira","family":"da Silva","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2016,3,28]]},"reference":[{"key":"S0967199416000071_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-133X(03)00121-2"},{"key":"S0967199416000071_ref002","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(94)77149-6"},{"key":"S0967199416000071_ref017","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/der110"},{"key":"S0967199416000071_ref057","doi-asserted-by":"publisher","DOI":"10.4161\/epi.21107"},{"key":"S0967199416000071_ref046","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1220737"},{"key":"S0967199416000071_ref011","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(93)77629-8"},{"key":"S0967199416000071_ref055","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-12"},{"key":"S0967199416000071_ref050","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(02)74367-1"},{"key":"S0967199416000071_ref030","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.018168"},{"key":"S0967199416000071_ref019","doi-asserted-by":"publisher","DOI":"10.1530\/REP-10-0055"},{"key":"S0967199416000071_ref006","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.4.1050"},{"key":"S0967199416000071_ref059","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2004.00493.x"},{"key":"S0967199416000071_ref038","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.14.0480"},{"key":"S0967199416000071_ref022","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.09-077M"},{"key":"S0967199416000071_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.02.011"},{"key":"S0967199416000071_ref062","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2010-4137"},{"key":"S0967199416000071_ref060","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(84)90241-6"},{"key":"S0967199416000071_ref053","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01143.2001"},{"key":"S0967199416000071_ref007","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2014.15.2.225"},{"key":"S0967199416000071_ref036","first-page":"208","article-title":"Heat shock-induced damage in bovine oocytes","volume":"43","author":"Paula-Lopes","year":"2008","journal-title":"Reprod. 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