{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T21:59:31Z","timestamp":1747173571293,"version":"3.40.5"},"reference-count":43,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2018,12,5]],"date-time":"2018-12-05T00:00:00Z","timestamp":1543968000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The aim of this study was to compare different concentrations of soy lecithin (LEC0.01%, LEC0.05% and LEC0.1%) with egg yolk (Control) in cooling extenders during the storage of semen at 5\u00baC for 5 days. Twelve dogs (<jats:italic>n<\/jats:italic>= 12) were selected, and semen was cooled and assessed after 2, 24, 48, 72, 96 or 120 h. At each time point, sperm were analyzed for kinetic patterns (using computer-assisted sperm analysis), mitochondrial activity (3\u20323- diaminobenzidine assay), lipid peroxidation (TBARS assay), DNA fragmentation (SCSA\u00ae) and plasma and acrosome membrane integrity (eosin\/nigrosin and fast green\/rose Bengal stains, respectively). The Control group (1814.4 \u00b1 197.2) presented the highest rates of lipid peroxidation at 120 h. Conversely, progressive motility (42.8 \u00b1 4%), linearity (45.4 \u00b1 1%), and VAP (88 \u00b1 3%) were higher in the Control group. In addition, there was lower mitochondrial activity in the Control group at 72 h. Therefore, our data show that lecithin used at these concentrations was not able to maintain sperm viability at as high qualities as would egg yolk. Moreover, the decrease in high mitochondrial activity and the persistence of sperm motility may indicate a compensatory mechanism in canine spermatozoa (i.e., glycolytic pathway). Furthermore, these higher lipid peroxidation indexes could indicate the necessity for future therapy using extenders and antioxidants over a long cooling time for dog sperm.<\/jats:p>","DOI":"10.1017\/s0967199418000576","type":"journal-article","created":{"date-parts":[[2018,12,5]],"date-time":"2018-12-05T16:07:21Z","timestamp":1544026041000},"page":"17-24","source":"Crossref","is-referenced-by-count":6,"title":["Insights into soy lecithin and egg yolk-based extenders for chilling canine spermatozoa"],"prefix":"10.1017","volume":"27","author":[{"given":"Andressa","family":"Dalmazzo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"de Souza Ramos Angrimani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7947-4027","authenticated-orcid":false,"given":"Jo\u00e3o Diego A.","family":"Losano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina C.","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos A. B.","family":"Sobrinho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Rafael","family":"Chinait Gurgel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro Ivo","family":"Monteiro Pacheco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Kiyomi","family":"Minazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia E.","family":"Crusco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcilio","family":"Nichi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valqu\u00edria H.","family":"Barnabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2018,12,5]]},"reference":[{"key":"S0967199418000576_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.10.015"},{"key":"S0967199418000576_ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01410.x"},{"key":"S0967199418000576_ref42","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2010.10.011"},{"key":"S0967199418000576_ref40","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(95)00278-G"},{"key":"S0967199418000576_ref10","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1002\/j.1939-4640.1994.tb00484.x","article-title":"Phosphatidylcholine enhances the acrosomal responsiveness of human sperm","volume":"15","author":"Cross","year":"1994","journal-title":"J Androl"},{"key":"S0967199418000576_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(78)52032-6"},{"key":"S0967199418000576_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00435-6"},{"key":"S0967199418000576_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.08.012"},{"key":"S0967199418000576_ref27","doi-asserted-by":"publisher","DOI":"10.1530\/REP-15-0178"},{"key":"S0967199418000576_ref16","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2605.1998.00106.x"},{"key":"S0967199418000576_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.10.005"},{"key":"S0967199418000576_ref41","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.08.091"},{"key":"S0967199418000576_ref15","doi-asserted-by":"publisher","DOI":"10.1136\/vr.102806"},{"key":"S0967199418000576_ref4","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12675"},{"key":"S0967199418000576_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.11.018"},{"key":"S0967199418000576_ref19","first-page":"457","article-title":"Cytochemical and ultracytochemical demonstration of cytochrome c oxidase in spermatozoa and dynamics of its changes accompanying ageing or induced by stress","volume":"11","author":"Hrudka","year":"1987","journal-title":"Int J Androl"},{"key":"S0967199418000576_ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-009-9249-9"},{"key":"S0967199418000576_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2012.10.024"},{"key":"S0967199418000576_ref29","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12901"},{"key":"S0967199418000576_ref14","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.20004"},{"key":"S0967199418000576_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.04.003"},{"key":"S0967199418000576_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.08.002"},{"key":"S0967199418000576_ref12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2011.10813.x"},{"key":"S0967199418000576_ref30","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/1682393"},{"key":"S0967199418000576_ref43","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2016.05.001"},{"key":"S0967199418000576_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.08.009"},{"key":"S0967199418000576_ref33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2012.02152.x"},{"key":"S0967199418000576_ref22","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1002\/j.1939-4640.2000.tb03273.x","article-title":"DNA integrity in human spermatozoa: relationships with semen quality","volume":"21","author":"Irvine","year":"2000","journal-title":"J Androl"},{"key":"S0967199418000576_ref35","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21549"},{"key":"S0967199418000576_ref3","doi-asserted-by":"publisher","DOI":"10.1111\/and.12154"},{"key":"S0967199418000576_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2009.05.026"},{"key":"S0967199418000576_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90738-3"},{"key":"S0967199418000576_ref38","first-page":"87","article-title":"A simple staining method for evaluating acrosomal status of cat spermatozoa","volume":"22","author":"Pope","year":"1991","journal-title":"J Zoo Wildlife Med"},{"key":"S0967199418000576_ref26","first-page":"998","article-title":"On the eosin-nigrosin stain and some other methods for the appraisal of sperm vitality with special reference to practical application","volume":"75","author":"Lagergren","year":"1953","journal-title":"Ann Ostet Ginecol"},{"key":"S0967199418000576_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.02.020"},{"key":"S0967199418000576_ref24","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0520167"},{"key":"S0967199418000576_ref11","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009844109023"},{"key":"S0967199418000576_ref34","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.026054"},{"key":"S0967199418000576_ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2016.02.001"},{"key":"S0967199418000576_ref1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gap059"},{"key":"S0967199418000576_ref7","doi-asserted-by":"publisher","DOI":"10.1080\/10495398.2016.1203797"},{"key":"S0967199418000576_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00440-X"},{"key":"S0967199418000576_ref18","first-page":"72","article-title":"Cryopreservation of dog semen using a soybean lecithin-based extender: effect on sperm viability and acrosome integrity","volume":"49","author":"Hidalgo","year":"2014","journal-title":"Reprod Domest Anim"}],"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0967199418000576","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T01:36:27Z","timestamp":1720834587000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0967199418000576\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,5]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["S0967199418000576"],"URL":"https:\/\/doi.org\/10.1017\/s0967199418000576","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"type":"print","value":"0967-1994"},{"type":"electronic","value":"1469-8730"}],"subject":[],"published":{"date-parts":[[2018,12,5]]}}}