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Five surplus donor oocytes were processed for transmission electron microscopy (TEM), and a stereological analysis was used to quantify the distribution of organelles, using the point-counting technique with an adequate stereological grid. Comparisons between means of the relative volumes (Vv) occupied by organelles in the three oocyte regions, cortex (C), subcortex (SC) and inner cytoplasm (IC), followed the Kruskal\u2013Wallis test and Mann\u2013Whitney<jats:italic>U<\/jats:italic>-test with Bonferroni correction. Life cell imaging and TEM analysis confirmed donor oocyte nuclear maturity. Results showed that the most abundant organelles were smooth endoplasmic reticulum (SER) elements (26.8%) and mitochondria (5.49%). Significant differences between oocyte regions were found for lysosomes (<jats:italic>P<\/jats:italic>= 0.003), cortical vesicles (<jats:italic>P<\/jats:italic>= 0.002) and large SER vesicles (<jats:italic>P<\/jats:italic>= 0.009). These results were quantitatively compared with previous results using prophase I (GV) and metaphase I (MI) immature oocytes. In donor MII oocytes there was a normal presence of cortical vesicles, SER tubules, SER small, medium and large vesicles, lysosomes and mitochondria. However, donor MII oocytes displayed signs of cytoplasmic immaturity, namely the presence of dictyosomes, present in GV oocytes and rare in MI oocytes, of SER very large vesicles, characteristic of GV oocytes, and the rarity of SER tubular aggregates. Results therefore indicate that the criterion of nuclear maturity used for donor oocyte selection does not always correspond to cytoplasmic maturity, which can partially explain implantation failures with the use of donor oocytes.<\/jats:p>","DOI":"10.1017\/s0967199423000114","type":"journal-article","created":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T08:43:30Z","timestamp":1679301810000},"page":"253-265","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":3,"title":["All that glitters is not gold: a stereological study of human donor oocytes"],"prefix":"10.1017","volume":"31","author":[{"given":"T\u00e2nia","family":"Santos","sequence":"first","affiliation":[]},{"given":"Ana 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