{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:52:27Z","timestamp":1760298747622},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2013,6,24]],"date-time":"2013-06-24T00:00:00Z","timestamp":1372032000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsc Microanal"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tuberculosis (TB) is a major health problem. The emergence of multidrug resistant (MDR)<jats:italic>Mycobacterium tuberculosis<\/jats:italic>(Mtb) isolates confounds treatment strategies. In Portugal, cases of MDR-TB are reported annually with an increased incidence noted in Lisbon. The majority of these MDR-TB cases are due to closely related mycobacteria known collectively as the<jats:italic>Lisboa<\/jats:italic>family and Q1 cluster. Genetic determinants linked to drug resistance have been exhaustively studied resulting in the identification of family and cluster specific mutations. Nevertheless, little is known about other factors involved in development of mycobacteria drug resistance. Here, we complement genetic analysis with the study of morphological and structural features of the<jats:italic>Lisboa<\/jats:italic>family and Q1 cluster isolates by using scanning and transmission electron microscopy. This analysis allowed the identification of structural differences, such as cell envelope thickness, between Mtb clinical isolates that are correlated with antibiotic resistance. The infection of human monocyte derived macrophages allowed us to document the relative selective advantage of the<jats:italic>Lisboa<\/jats:italic>family isolates over other circulating Mtb isolates.<\/jats:p>","DOI":"10.1017\/s1431927613001906","type":"journal-article","created":{"date-parts":[[2013,6,24]],"date-time":"2013-06-24T08:15:37Z","timestamp":1372061737000},"page":"1159-1169","source":"Crossref","is-referenced-by-count":6,"title":["Exploring the Contribution of Mycobacteria Characteristics in Their Interaction with Human Macrophages"],"prefix":"10.1093","volume":"19","author":[{"given":"Carla","family":"Silva","sequence":"first","affiliation":[]},{"given":"Joao","family":"Perdigao","sequence":"additional","affiliation":[]},{"given":"Elsa","family":"Alverca","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio P. Alves","family":"de Matos","sequence":"additional","affiliation":[]},{"given":"Patricia A.","family":"Carvalho","sequence":"additional","affiliation":[]},{"given":"Isabel","family":"Portugal","sequence":"additional","affiliation":[]},{"given":"Luisa","family":"Jordao","sequence":"additional","affiliation":[]}],"member":"286","published-online":{"date-parts":[[2013,6,24]]},"reference":[{"key":"S1431927613001906_ref012","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.2170-2176.2004"},{"key":"S1431927613001906_ref034","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2003.08.009"},{"key":"S1431927613001906_ref018","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00940.x"},{"key":"S1431927613001906_ref021","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610634104"},{"key":"S1431927613001906_ref020","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054926"},{"key":"S1431927613001906_ref031","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.2.571-577.2005"},{"key":"S1431927613001906_ref043","first-page":"437","volume-title":"Lung Macrophages and Dendritic Cells in Health and Disease","author":"Schlesinger","year":"1997"},{"key":"S1431927613001906_ref017","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27629-0"},{"key":"S1431927613001906_ref046","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01392-06"},{"key":"S1431927613001906_ref053","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.55.1.139"},{"key":"S1431927613001906_ref011","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00016-10"},{"key":"S1431927613001906_ref035","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2008.0798"},{"key":"S1431927613001906_ref002","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-007-0386-0"},{"key":"S1431927613001906_ref041","doi-asserted-by":"publisher","DOI":"10.1590\/S0034-89102002000400021"},{"key":"S1431927613001906_ref033","doi-asserted-by":"crossref","first-page":"2452","DOI":"10.1128\/AAC.40.11.2452","article-title":"Correlation of molecular resistance mechanisms and phenotypic resistance levels in streptomycin-resistant Mycobacterium tuberculosis","volume":"40","author":"Meier","year":"1996","journal-title":"Antimicrob Agents Chemother"},{"key":"S1431927613001906_ref040","doi-asserted-by":"publisher","DOI":"10.1054\/tuld.1998.0002"},{"key":"S1431927613001906_ref028","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.9.2971-2973.2003"},{"key":"S1431927613001906_ref056","unstructured":"World Health Organization (WHO) (2012). Global tuberculosis report 2012. http:\/\/apps.who.int\/iris\/bitstream\/10665\/75938\/1\/9789241564502_eng.pdf (accessed February 1, 2013)."},{"key":"S1431927613001906_ref048","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cws078"},{"key":"S1431927613001906_ref049","doi-asserted-by":"publisher","DOI":"10.1159\/000226425"},{"key":"S1431927613001906_ref022","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1994.tb07194.x"},{"key":"S1431927613001906_ref037","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks371"},{"key":"S1431927613001906_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-9792(02)00089-6"},{"key":"S1431927613001906_ref001","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.593"},{"key":"S1431927613001906_ref025","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1128\/JCM.32.4.1095-1098.1994","article-title":"Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin resistant Mycobacterium tuberculosis strains from New York City and Texas","volume":"32","author":"Kapur","year":"1994","journal-title":"J Clin Microbiol"},{"key":"S1431927613001906_ref023","first-page":"529","article-title":"On the killing of mycobacteria by macrophages","volume":"10","author":"Jordao","year":"2008","journal-title":"Cell Microbiol"},{"key":"S1431927613001906_ref036","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp452"},{"key":"S1431927613001906_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/S0277-9536(96)00143-8"},{"key":"S1431927613001906_ref050","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00064510"},{"key":"S1431927613001906_ref026","doi-asserted-by":"publisher","DOI":"10.1016\/0041-3879(87)90064-X"},{"key":"S1431927613001906_ref039","first-page":"207","article-title":"Outbreak of multiple drug-resistant tuberculosis in Lisbon: Detection by restriction fragment length polymorphism analysis","volume":"3","author":"Portugal","year":"1999","journal-title":"Int J Tuberc Lung Dis"},{"key":"S1431927613001906_ref047","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2447"},{"key":"S1431927613001906_ref051","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01515-07"},{"key":"S1431927613001906_ref055","unstructured":"World Health Organization (WHO) (2011). 2011\/2012 tuberculosis global facts. http:\/\/www.who.int\/tb\/publications\/2011\/factsheet_tb_2011.pdf (accessed February 1, 2013)."},{"key":"S1431927613001906_ref003","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.023028-0"},{"key":"S1431927613001906_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2009.04.001"},{"key":"S1431927613001906_ref005","doi-asserted-by":"publisher","DOI":"10.3109\/10520297309116648"},{"key":"S1431927613001906_ref007","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1002\/pro.324","article-title":"Isoniazid-resistance conferring mutations in Mycobacterium tuberculosis KatG: Catalase, peroxidase, and INH-NADH adduct formation activities","volume":"19","author":"Cade","year":"2010","journal-title":"Protein Sci"},{"key":"S1431927613001906_ref008","doi-asserted-by":"publisher","DOI":"10.1126\/science.339.6116.130"},{"key":"S1431927613001906_ref032","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3192-3197.2005"},{"key":"S1431927613001906_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.07.022"},{"key":"S1431927613001906_ref010","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1128\/JB.180.4.801-808.1998","article-title":"Mycobacterial stationary phase induced by low oxygen tension: Cell wall thickening and localization of the 16-kilodalton alpha-crystallin homolog","volume":"180","author":"Cunningham","year":"1998","journal-title":"J Bacteriol"},{"key":"S1431927613001906_ref013","first-page":"405","volume-title":"Tuberculosis and the Tubercle Bacillus","author":"Drapper","year":"2005"},{"key":"S1431927613001906_ref014","doi-asserted-by":"publisher","DOI":"10.1038\/nm0307-284"},{"key":"S1431927613001906_ref016","first-page":"405","volume-title":"Tuberculosis and the Tubercle Bacillus","author":"Fenton","year":"2005"},{"key":"S1431927613001906_ref019","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124410"},{"key":"S1431927613001906_ref024","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/403623"},{"key":"S1431927613001906_ref027","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1128\/AAC.40.8.1768","article-title":"Gyrase mutations in laboratory-selected, fluoroquinolones-resistant mutations of Mycobacterium tuberculosis H37Ra","volume":"40","author":"Kocag\u00f6z","year":"1996","journal-title":"Antimicrob Agents Chemother"},{"key":"S1431927613001906_ref029","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2004.07.016"},{"key":"S1431927613001906_ref030","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03162.x"},{"key":"S1431927613001906_ref038","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.5.1900-1902.2006"},{"key":"S1431927613001906_ref042","doi-asserted-by":"publisher","DOI":"10.1038\/35085034"},{"key":"S1431927613001906_ref044","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1128\/AAC.40.4.1024","article-title":"Characterization of rpsl and rrs mutations in streptomycin-resistant Mycobacterium tuberculosis isolates from diverse geographic localities","volume":"40","author":"Sreevatsan","year":"1996","journal-title":"Antimicrob Agents Chemother"},{"key":"S1431927613001906_ref045","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1128\/AAC.41.3.636","article-title":"Mutations associated with pyrazinamide resistance in pncA of Mycobacterium tuberculosis complex organisms","volume":"41","author":"Sreevatsan","year":"1997","journal-title":"Antimicrob Agents Chemother"},{"key":"S1431927613001906_ref052","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1000824"},{"key":"S1431927613001906_ref057","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907925106"},{"key":"S1431927613001906_ref054","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq351"}],"container-title":["Microscopy and Microanalysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S1431927613001906","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T21:03:27Z","timestamp":1676408607000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/mam\/article\/19\/5\/1159\/6932641"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,24]]},"references-count":57,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["S1431927613001906"],"URL":"https:\/\/doi.org\/10.1017\/s1431927613001906","relation":{},"ISSN":["1431-9276","1435-8115"],"issn-type":[{"value":"1431-9276","type":"print"},{"value":"1435-8115","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,24]]}}}