{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T12:55:26Z","timestamp":1754398526860,"version":"3.40.5"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T00:00:00Z","timestamp":1553731200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsc Microanal"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The adsorption of intact liposomes on surfaces is of great importance for the development of sensors and drug delivery systems and, also, strongly dependent on the surface roughness where the liposomes are adsorbed. In this paper, we analyzed, by using atomic force microscopy in liquid, the evolution of the morphology of gold surfaces and of poly(allylamine hydrochloride) (PAH) surfaces with different roughness during the adsorption of liposomes prepared with the synthetic phospholipid 1,2-dipalmitoyl-<jats:italic>sn<\/jats:italic>-glycero-3-[phospho-rac-(1-glycerol)]. Our results reveal the following. On smooth surfaces of Au only and Au with PAH, the liposomes open and deploy on the substrate, creating a supported-lipid bilayer, with the opening process being faster on the Au\/PAH surface. On rough substrates of Au coated with polyelectrolyte multilayers, the liposomes were adsorbed intact on the surface. This was corroborated by power spectral density analysis that demonstrates the presence of superstructures with an average lateral size of 43 and 87 nm, in accordance with two and four times the mean liposome hydrodynamic diameter of about 21 nm. In addition, this work presents an adequate and effective methodology for analysis of adsorption phenomena of liposomes on rough surfaces.<\/jats:p>","DOI":"10.1017\/s1431927619000345","type":"journal-article","created":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T09:46:39Z","timestamp":1553766399000},"page":"798-809","source":"Crossref","is-referenced-by-count":4,"title":["<i>In Situ<\/i> AFM Imaging of Adsorption Kinetics of DPPG Liposomes: A Quantitative Analysis of Surface Roughness"],"prefix":"10.1093","volume":"25","author":[{"given":"Andreia A.","family":"Duarte","sequence":"first","affiliation":[]},{"given":"Joaquim T.","family":"Marqu\u00eas","sequence":"additional","affiliation":[]},{"given":"Francisco","family":"Brasil","sequence":"additional","affiliation":[]},{"given":"Ana S.","family":"Viana","sequence":"additional","affiliation":[]},{"given":"Pedro","family":"Tavares","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4710-0693","authenticated-orcid":false,"given":"Maria","family":"Raposo","sequence":"additional","affiliation":[]}],"member":"286","published-online":{"date-parts":[[2019,3,28]]},"reference":[{"doi-asserted-by":"publisher","key":"S1431927619000345_ref12","DOI":"10.1140\/epje\/i2013-13098-7"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref26","DOI":"10.1016\/j.bbamem.2010.10.006"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref21","DOI":"10.1116\/1.579869"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref6","DOI":"10.1016\/S0924-4247(01)00637-9"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref17","DOI":"10.1021\/la304036h"},{"volume-title":"Fractal Growth Phenomena","year":"1992","author":"Viscek","key":"S1431927619000345_ref32"},{"key":"S1431927619000345_ref3","first-page":"71","article-title":"The optimal estimation of finish parameters","volume":"1530","author":"Church","year":"1991","journal-title":"SPIE"},{"key":"S1431927619000345_ref27","first-page":"2244","article-title":"Quantitative topographic analysis of fractal surfaces by scanning tunneling microscopy","volume":"5","author":"Mitchell","year":"1990","journal-title":"J Mater Sci"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref5","DOI":"10.1021\/jp983996x"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref25","DOI":"10.1016\/j.jcis.2007.04.040"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref30","DOI":"10.1186\/1475-925X-9-59"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref31","DOI":"10.1007\/BF01337937"},{"key":"S1431927619000345_ref4","first-page":"136","article-title":"The prediction of BRDFs from surface profile measurements","volume":"1165","author":"Church","year":"1989","journal-title":"SPIE"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref7","DOI":"10.1016\/j.apsusc.2009.08.046"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref24","DOI":"10.1016\/0967-0645(95)00008-E"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref1","DOI":"10.1021\/la900181c"},{"key":"S1431927619000345_ref18","first-page":"291","article-title":"A power spectral density study of thin films morphology based on AFM profiling","volume":"10","author":"Gavrila","year":"2007","journal-title":"Rom J Inf Sci Technol"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref10","DOI":"10.1109\/ENBENG.2012.6331368"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref16","DOI":"10.1021\/jp908632a"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref33","DOI":"10.1017\/CBO9780511599798"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref28","DOI":"10.1016\/0022-4898(91)90030-A"},{"key":"S1431927619000345_ref11","first-page":"1","article-title":"Adsorption kinetics of DPPG liposome layers: A quantitative analysis of surface roughness","volume":"7","author":"Duarte","year":"2013","journal-title":"Microsc Microanal"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref14","DOI":"10.1063\/1.4921715"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref23","DOI":"10.1021\/es00069a009"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref22","DOI":"10.1016\/S0043-1354(01)00281-0"},{"unstructured":"Dittman, MG (2006). K-correlation power spectral density and surface scatter model. In Proc. SPIE 6291, Optical Systems Degradation, Contamination, and Stray Light: Effects, Measurements, and Control II, 62910R.","key":"S1431927619000345_ref9"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref15","DOI":"10.1364\/AO.40.002190"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref13","DOI":"10.1021\/acs.jpcb.5b02384"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref20","DOI":"10.1016\/j.apsusc.2007.01.056"},{"key":"S1431927619000345_ref29","first-page":"183","volume-title":"Microscopy and Imaging Science: Practical Approaches to Applied Research and Education","author":"Pires","year":"2017"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref8","DOI":"10.1126\/science.277.5330.1232"},{"unstructured":"Iazykov, M (2011). Growth of pentacene on parylene and on BCB for organic transistors application, and DNA-based nanostructures studied by Amplitude: Modulation Atomic Force Microscopy in air and in liquids. Ecole Centrale de Lyon.","key":"S1431927619000345_ref19"},{"doi-asserted-by":"publisher","key":"S1431927619000345_ref2","DOI":"10.1021\/ma048596o"}],"container-title":["Microscopy and Microanalysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S1431927619000345","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T21:31:43Z","timestamp":1676410303000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/mam\/article\/25\/3\/798\/6887475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,28]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["S1431927619000345"],"URL":"https:\/\/doi.org\/10.1017\/s1431927619000345","relation":{},"ISSN":["1431-9276","1435-8115"],"issn-type":[{"type":"print","value":"1431-9276"},{"type":"electronic","value":"1435-8115"}],"subject":[],"published":{"date-parts":[[2019,3,28]]}}}