{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T18:45:20Z","timestamp":1749581120511},"reference-count":15,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2011,3,16]],"date-time":"2011-03-16T00:00:00Z","timestamp":1300233600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plant Genet. Res."],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:p>Woad (<jats:italic>Isatis tinctoria<\/jats:italic> L.) was introduced in Europe in ancient times to produce indigo, a natural blue pigment used mainly for dyestuff. This species was cultivated in Portugal until the beginning of the 20th century, especially in the inner North and South. A set of nine inter-simple sequence repeat (ISSR) markers generated 177 reproducible fragments, of which 171 were polymorphic. The mean number of fragments\/accession was 111, ranging between 100 (Portugal-Coimbra) and 124 (Poland). The total polymorphism observed was 0.3272, the average polymorphism was 0.1784 and the gene differentiation between accessions was 0.4546. Polymorphism ranged between 53.8% (Austria) and 73.1% (Belgium). The genetic relationship among woad accessions was obtained with unweighted pair group method with arithmetic mean dendrogram based on a molecular marker, clearly clustering the woad accessions according to their geographic origin. The genetic diversity observed in this collection shows that there is a considerable potential for its improvement and that ISSR could be used to evaluate intra- and inter-accession similarities in <jats:italic>I. tinctoria<\/jats:italic> species.<\/jats:p>","DOI":"10.1017\/s1479262111000463","type":"journal-article","created":{"date-parts":[[2011,3,16]],"date-time":"2011-03-16T07:19:49Z","timestamp":1300259989000},"page":"210-213","source":"Crossref","is-referenced-by-count":3,"title":["Genetic diversity in woad (<i>Isatis tinctoria<\/i> L.) accessions detected by ISSR markers"],"prefix":"10.1017","volume":"9","author":[{"given":"Lu\u00eds","family":"Rocha","sequence":"first","affiliation":[]},{"given":"Sandra","family":"Martins","sequence":"additional","affiliation":[]},{"given":"Valdemar","family":"Carnide","sequence":"additional","affiliation":[]},{"given":"Fernando","family":"Braga","sequence":"additional","affiliation":[]},{"given":"Carlos","family":"Carvalho","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2011,3,16]]},"reference":[{"key":"S1479262111000463_ref1","first-page":"467","article-title":"Application of inter simple sequence repeat (ISSR marker) to detect genotoxic effect of heavy metals on Eruca sativa (L.)","volume":"9","author":"Al-Qurainy","year":"2010","journal-title":"African Journal of Biotechnology"},{"key":"S1479262111000463_ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s10722-006-0014-4"},{"key":"S1479262111000463_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2004.10.014"},{"key":"S1479262111000463_ref7","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1007\/s001220051602","article-title":"Genetic relationships among species of the genus Diplotaxis (Brassicaceae) using inter-simple sequence repeat markers","volume":"101","author":"Mart\u00edn","year":"2000","journal-title":"Theoretical and Applied Genetics"},{"key":"S1479262111000463_ref4","doi-asserted-by":"publisher","DOI":"10.1023\/A:1013374618870"},{"key":"S1479262111000463_ref14","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006042331385"},{"key":"S1479262111000463_ref9","doi-asserted-by":"publisher","DOI":"10.1007\/s002170100367"},{"key":"S1479262111000463_ref11","first-page":"221","article-title":"Characteristics and a comparison of three classes of microsatellite-based markers and their application in plants","volume":"9","author":"Rakoczy-Trojanowska","year":"2004","journal-title":"Cellular and Molecular Biology Letters"},{"key":"S1479262111000463_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2113(08)60578-7"},{"key":"S1479262111000463_ref5","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1007\/s00122-001-0863-3","article-title":"A high degree of genetic diversity is revealed in Isatis spp. (dyer's woad) by amplified fragment length polymorphism (AFLP)","volume":"104","author":"Gilbert","year":"2002","journal-title":"Theoretical and Applied Genetics"},{"key":"S1479262111000463_ref12","doi-asserted-by":"publisher","DOI":"10.1007\/s10681-007-9604-2"},{"key":"S1479262111000463_ref15","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1994.1151"},{"key":"S1479262111000463_ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s10722-008-9354-6"},{"key":"S1479262111000463_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2009.06.017"},{"key":"S1479262111000463_ref3","first-page":"38","volume-title":"Chromosome Atlas of Flowering Plants","author":"Darlington","year":"1955"}],"container-title":["Plant Genetic Resources"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S1479262111000463","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,27]],"date-time":"2019-04-27T04:03:15Z","timestamp":1556337795000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S1479262111000463\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3,16]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,7]]}},"alternative-id":["S1479262111000463"],"URL":"https:\/\/doi.org\/10.1017\/s1479262111000463","relation":{},"ISSN":["1479-2621","1479-263X"],"issn-type":[{"value":"1479-2621","type":"print"},{"value":"1479-263X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,3,16]]}}}