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Employing molecular dynamics (MD) simulations coupled with enhanced sampling, we map the structural thermodynamics of pore nucleation across multiple lipid species and Chol concentrations, analyzing 99 membrane systems (33 zero- and 66 applied-field) totaling &amp;gt;0.40 ms of MD sampling. We reveal that pore-induced acyl-chain interdigitation acts as a thermodynamic barrier, forcibly expelling rigid Chol. Whole-membrane z-profiles of membrane potential and water-dipole orientation largely mirror bulk behavior, showing that global averaging masks the pore-core electrostatic response and necessitating characteristic-radius-resolved local sampling. Crucially, high applied potentials induce an electro-topological disparity: voltage-driven leaflet interpenetration structurally outpaces aqueous pore dilation. Radius-resolved electrostatic matrices, phenotype clustering, and state-progression analyses show that interdigitation-defined local domains concentrate core water ordering and potential amplification. Rather than a simple nanoscale hole, this extensive electro-deformation generates an expanded \u201clipid scar\u201d characterized by substantial tail entanglement, sharp lateral sterol gradients, and localized electrostatic enrichment. This amplified structural prestrain creates a thermodynamically vulnerable domain, potentially lowering the activation energy for consecutive poration, clarifying the molecular origins of electrosensitization in pulsed electric field therapies.<\/jats:p>","DOI":"10.1021\/acs.jcim.6c01534","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T19:51:36Z","timestamp":1784749896000},"page":"9396-9407","source":"Crossref","is-referenced-by-count":0,"title":["Structural Thermodynamics\nof Membrane Electroporation:\nVoltage-Driven Interdigitation Induces Electro-Topological Disparity"],"prefix":"10.1021","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6035-8414","authenticated-orcid":true,"given":"Xin","family":"Song","sequence":"first","affiliation":[{"name":"Chongqing University of Posts and Telecommunications , , ,","place":["Chongqing, China, 400065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7230-018X","authenticated-orcid":true,"given":"Fei","family":"Guo","sequence":"additional","affiliation":[{"name":"Chongqing University of Posts and Telecommunications , , ,","place":["Chongqing, China, 400065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilin","family":"Dan","sequence":"additional","affiliation":[{"name":"Chongqing University of Posts and Telecommunications , , ,","place":["Chongqing, China, 400065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Zhu","sequence":"additional","affiliation":[{"name":"Chongqing University of Posts and Telecommunications , , ,","place":["Chongqing, China, 400065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chongqing Hospital of Traditional Chinese Medicine , , ,","place":["Chongqing, China, 400013"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"issue":"11","key":"2026081008104585200_cit1","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1007\/s11745-004-1336-x","article-title":"What\u2019s so Special about Cholesterol?","volume":"39","author":"Mouritsen","year":"2004","journal-title":"Lipids"},{"issue":"3","key":"2026081008104585200_cit2","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/S0304-4157(00)00016-2","article-title":"Structure of Lipid Bilayers","volume":"1469","author":"Nagle","year":"2000","journal-title":"Biochim. 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Acta Biomembr."},{"issue":"2","key":"2026081008104585200_cit5","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/j.bbamem.2015.12.003","article-title":"Interdigitation\nof Long-Chain Sphingomyelin Induces Coupling of Membrane Leaflets\nin a Cholesterol Dependent Manner","volume":"1858","author":"Ro\u0301g","year":"2016","journal-title":"Biochim.\nBiophys. Acta, Biomembr."},{"issue":"1\u20132","key":"2026081008104585200_cit6","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/s0009-3084(99)00071-7","article-title":"Sphingomyelin\u2013Cholesterol\nInteractions in Biological and Model Membranes","volume":"102","author":"Slotte","year":"1999","journal-title":"Chem. Phys. 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