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Thermal fluctuations are responsible for transitions between these conformers. Normal-modes analysis (NMA) using elastic network models (ENMs) provides an efficient procedure to explore global dynamics of proteins commonly associated with conformational transitions. In the present work, we present an iterative approach to explore protein conformational spaces by introducing structural distortions according to their equilibrium dynamics at room temperature. The approach can be used either to perform unbiased explorations of conformational space or to explore guided pathways connecting two different conformations, e.g., apo and holo forms. In order to test its performance, four proteins with different magnitudes of structural distortions upon ligand binding have been tested. In all cases, the conformational selection model has been confirmed and the conformational space between apo and holo forms has been encompassed. Different strategies have been tested that impact on the efficiency either to achieve a desired conformational change or to achieve a balanced exploration of the protein conformational multiplicity.<\/jats:p>","DOI":"10.1021\/acs.jcim.9b01136","type":"journal-article","created":{"date-parts":[[2020,3,27]],"date-time":"2020-03-27T11:06:23Z","timestamp":1585307183000},"page":"3068-3080","source":"Crossref","is-referenced-by-count":6,"title":["Exploring Conformational Space with Thermal Fluctuations\nObtained by Normal-Mode Analysis"],"prefix":"10.1021","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0017-4660","authenticated-orcid":true,"given":"Tadeo\nE.","family":"Saldan\u0303o","sequence":"first","affiliation":[{"name":"Universidad Nacional de Quilmes\/CONICET , Roque Saenz Pen\u0303a 352 , ,","place":["Bernal, Argentina, B1876BXD"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor M.","family":"Freixas","sequence":"additional","affiliation":[{"name":"Universidad Nacional de Quilmes\/CONICET , Roque Saenz Pen\u0303a 352 , ,","place":["Bernal, Argentina, B1876BXD"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvio C. E.","family":"Tosatto","sequence":"additional","affiliation":[{"name":"University of Padova , , Viale G. 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