{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T22:18:06Z","timestamp":1759961886418,"version":"3.37.3"},"reference-count":68,"publisher":"American Chemical Society (ACS)","issue":"10","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21173247","31270785"],"award-info":[{"award-number":["21173247","31270785"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Director Innovation Foundation, Qingdao Institute of Bioenergy and Bioprocess Technology"},{"name":"Outstanding Young Scientist Foundation of Shandong Province","award":["JQ201104","ZR2011BQ008"],"award-info":[{"award-number":["JQ201104","ZR2011BQ008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Chem. Inf. Model."],"published-print":{"date-parts":[[2014,10,27]]},"DOI":"10.1021\/ci500339v","type":"journal-article","created":{"date-parts":[[2014,10,6]],"date-time":"2014-10-06T15:54:54Z","timestamp":1412610894000},"page":"2826-2833","source":"Crossref","is-referenced-by-count":19,"title":["Improving <i>Trichoderma reesei<\/i> Cel7B Thermostability by Targeting the Weak Spots"],"prefix":"10.1021","volume":"54","author":[{"given":"Shujun","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory\rof Biofuels, Qingdao\rInstitute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences, Qingdao, 266061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yefei","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory\rof Biofuels, Qingdao\rInstitute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences, Qingdao, 266061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangfei","family":"Song","sequence":"additional","affiliation":[{"name":"Laboratory\rof Biofuels, Qingdao\rInstitute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences, Qingdao, 266061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingbo","family":"Hong","sequence":"additional","affiliation":[{"name":"Laboratory\rof Biofuels, Qingdao\rInstitute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences, Qingdao, 266061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory\rof Biofuels, Qingdao\rInstitute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences, Qingdao, 266061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lishan","family":"Yao","sequence":"additional","affiliation":[{"name":"Laboratory\rof Biofuels, Qingdao\rInstitute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences, Qingdao, 266061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"316","published-online":{"date-parts":[[2014,10,6]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1126\/science.1137016","volume":"315","author":"Himmel M. E.","year":"2007","journal-title":"Science"},{"key":"ref2\/cit2","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1042\/BJ20040892","volume":"382","author":"Boraston A. B.","year":"2004","journal-title":"Biochem. J."},{"key":"ref3\/cit3","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1023\/A:1009280109519","volume":"7","author":"Suurnakki A.","year":"2000","journal-title":"Cellulose"},{"key":"ref4\/cit4","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0168-1656(97)00087-4","volume":"57","author":"Linder M.","year":"1997","journal-title":"J. Biotechnol."},{"key":"ref5\/cit5","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.biortech.2013.05.079","volume":"143","author":"Le Costaouec T.","year":"2013","journal-title":"Bioresour. Technol."},{"key":"ref6\/cit6","first-page":"7","author":"Pakarinen A.","year":"2014","journal-title":"Biotechnol. Biofuels"},{"key":"ref7\/cit7","first-page":"6","author":"Varnai A.","year":"2013","journal-title":"Biotechnol. Biofuels"},{"key":"ref8\/cit8","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.jbiotec.2011.07.016","volume":"155","author":"Hall M.","year":"2011","journal-title":"J. Biotechnol."},{"key":"ref9\/cit9","doi-asserted-by":"crossref","first-page":"12809","DOI":"10.1073\/pnas.95.22.12809","volume":"95","author":"Giver L.","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref10\/cit10","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1038\/nmeth0109-3","volume":"6","author":"Benedix A.","year":"2009","journal-title":"Nat. Methods"},{"key":"ref11\/cit11","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.jmb.2004.12.019","volume":"347","author":"Pokala N.","year":"2005","journal-title":"J. Mol. Biol."},{"key":"ref12\/cit12","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0022-2836(02)00442-4","volume":"320","author":"Guerois R.","year":"2002","journal-title":"J. Mol. Biol."},{"key":"ref13\/cit13","doi-asserted-by":"crossref","first-page":"W306","DOI":"10.1093\/nar\/gki375","volume":"33","author":"Capriotti E.","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref14\/cit14","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1002\/prot.22921","volume":"79","author":"Kellogg E. H.","year":"2011","journal-title":"Proteins: Struct., Funct., Bioinf."},{"key":"ref15\/cit15","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1016\/j.jmb.2006.11.080","volume":"366","author":"Dantas G.","year":"2007","journal-title":"J. Mol. Biol."},{"key":"ref16\/cit16","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/B978-0-12-381270-4.00019-6","volume":"487","author":"Leaver-Fay A.","year":"2011","journal-title":"Method. Enzymol."},{"key":"ref17\/cit17","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1093\/protein\/gzt061","volume":"27","author":"Wijma H. J.","year":"2014","journal-title":"Protein Eng. Des. Sel."},{"key":"ref18\/cit18","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1093\/protein\/gzp030","volume":"22","author":"Potapov V.","year":"2009","journal-title":"Protein Eng., Des. Sel."},{"key":"ref19\/cit19","first-page":"9","author":"Song X. F.","year":"2013","journal-title":"PLoS Comput. Biol."},{"key":"ref20\/cit20","doi-asserted-by":"crossref","first-page":"2627","DOI":"10.1016\/S0006-3495(02)75604-X","volume":"82","author":"Losi A.","year":"2002","journal-title":"Biophys. J."},{"key":"ref21\/cit21","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.bioeng.2004.12.003","volume":"22","author":"Eijsink V. G. H.","year":"2005","journal-title":"Biomol. Eng."},{"key":"ref22\/cit22","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jbiotec.2004.03.026","volume":"113","author":"Eijsink V. G. H.","year":"2004","journal-title":"J. Biotechnol."},{"key":"ref23\/cit23","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1038\/nsb0595-374","volume":"2","author":"Eijsink V. G. H.","year":"1995","journal-title":"Nat. Struct. Biol."},{"key":"ref24\/cit24","doi-asserted-by":"crossref","first-page":"11152","DOI":"10.1074\/jbc.272.17.11152","volume":"272","author":"Mansfeld J.","year":"1997","journal-title":"J. Biol. Chem."},{"key":"ref25\/cit25","doi-asserted-by":"crossref","first-page":"35074","DOI":"10.1074\/jbc.273.52.35074","volume":"273","author":"Vriend G.","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref26\/cit26","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1006\/jmbi.2002.5446","volume":"317","author":"Cordier F.","year":"2002","journal-title":"J. Mol. Biol."},{"key":"ref27\/cit27","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s10858-012-9689-3","volume":"55","author":"Hong J. B.","year":"2013","journal-title":"J. Biomol. NMR"},{"key":"ref28\/cit28","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1038\/nchem.1396","volume":"4","author":"Nisius L.","year":"2012","journal-title":"Nat.Chem."},{"key":"ref29\/cit29","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1016\/j.jmb.2003.11.042","volume":"335","author":"Ding K. Y.","year":"2004","journal-title":"J. Mol. Biol."},{"key":"ref30\/cit30","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1093\/protein\/gzr029","volume":"24","author":"Wang T.","year":"2011","journal-title":"Protein Eng. Des. Sel."},{"key":"ref31\/cit31","doi-asserted-by":"crossref","first-page":"6680","DOI":"10.1021\/bi100929x","volume":"49","author":"Meharenna Y. T.","year":"2010","journal-title":"Biochemistry"},{"key":"ref32\/cit32","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1093\/protein\/15.3.185","volume":"15","author":"Pikkemaat M. G.","year":"2002","journal-title":"Protein Eng."},{"key":"ref33\/cit33","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1093\/protein\/gzp090","volume":"23","author":"Merkley E. D.","year":"2010","journal-title":"Protein Eng. Des. Sel."},{"key":"ref34\/cit34","doi-asserted-by":"crossref","first-page":"2451","DOI":"10.1529\/biophysj.106.082891","volume":"91","author":"Huang X.","year":"2006","journal-title":"Biophys. J."},{"key":"ref35\/cit35","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1002\/prot.21729","volume":"71","author":"Motono C.","year":"2008","journal-title":"Proteins: Struct., Funct., Bioinf."},{"key":"ref36\/cit36","first-page":"133","volume":"97","author":"Alonso D. O. V.","year":"2000","journal-title":"Eur. J. Med. Chem."},{"key":"ref37\/cit37","doi-asserted-by":"crossref","first-page":"13518","DOI":"10.1073\/pnas.250473497","volume":"97","author":"Mayor U.","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref38\/cit38","doi-asserted-by":"crossref","first-page":"13286","DOI":"10.1073\/pnas.1835776100","volume":"100","author":"Gianni S.","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref39\/cit39","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/j.jmb.2004.12.061","volume":"347","author":"Ferguson N.","year":"2005","journal-title":"J. Mol. Biol."},{"key":"ref40\/cit40","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1110\/ps.073004907","volume":"16","author":"Sharpe T.","year":"2007","journal-title":"Protein Sci."},{"key":"ref41\/cit41","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1006\/jmbi.1997.1243","volume":"272","author":"Kleywegt G. J.","year":"1997","journal-title":"J. Mol. Biol."},{"key":"ref42\/cit42","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1021\/ct100578z","volume":"7","author":"Olsson M. H. M.","year":"2011","journal-title":"J. Chem. Theory Comput."},{"key":"ref43\/cit43","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1021\/ct700301q","volume":"4","author":"Hess B.","year":"2008","journal-title":"J. Chem. Theory Comput."},{"key":"ref44\/cit44","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1002\/jcc.20291","volume":"26","author":"Van der Spoel D.","year":"2005","journal-title":"J. Comput. Chem."},{"key":"ref45\/cit45","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1002\/prot.21123","volume":"65","author":"Hornak V.","year":"2006","journal-title":"Proteins: Struct., Funct., Bioinf."},{"key":"ref46\/cit46","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1063\/1.445869","volume":"79","author":"Jorgensen W. L.","year":"1983","journal-title":"J. Chem. Phys."},{"key":"ref47\/cit47","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1080\/00268978300102851","volume":"50","author":"Nose S.","year":"1983","journal-title":"Mol. Phys."},{"key":"ref48\/cit48","doi-asserted-by":"crossref","first-page":"7182","DOI":"10.1063\/1.328693","volume":"52","author":"Parrinello M.","year":"1981","journal-title":"J. Appl. Phys."},{"key":"ref49\/cit49","first-page":"139","volume":"205","author":"Berendsen H. J. C.","year":"1991","journal-title":"Nato. Adv. Sci. I E-Appl."},{"key":"ref50\/cit50","doi-asserted-by":"crossref","first-page":"10089","DOI":"10.1063\/1.464397","volume":"98","author":"Darden T.","year":"1993","journal-title":"J. Chem. Phys."},{"key":"ref51\/cit51","doi-asserted-by":"crossref","first-page":"8577","DOI":"10.1063\/1.470117","volume":"103","author":"Essmann U.","year":"1995","journal-title":"J. Chem. Phys."},{"key":"ref52\/cit52","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1002\/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H","volume":"18","author":"Hess B.","year":"1997","journal-title":"J. Comput. Chem."},{"key":"ref53\/cit53","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1002\/jcc.540130805","volume":"13","author":"Miyamoto S.","year":"1992","journal-title":"J. Comput. Chem."},{"key":"ref54\/cit54","doi-asserted-by":"crossref","first-page":"8714","DOI":"10.1021\/jp403999s","volume":"117","author":"Zhang Y.","year":"2013","journal-title":"J. Phys. Chem. B"},{"key":"ref55\/cit55","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1042\/bj2700251","volume":"270","author":"Claeyssens M.","year":"1990","journal-title":"Biochem. J."},{"key":"ref56\/cit56","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1074\/jbc.M111.276485","volume":"287","author":"Murphy L.","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref57\/cit57","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1007\/s11274-009-0121-4","volume":"25","author":"Xue Y. M.","year":"2009","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref58\/cit58","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/0003-2697(77)90594-2","volume":"81","author":"Lever M.","year":"1977","journal-title":"Anal. Biochem."},{"key":"ref59\/cit59","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1021\/ac60147a030","volume":"31","author":"Miller G. L.","year":"1959","journal-title":"Anal. Chem."},{"key":"ref60\/cit60","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1002\/bit.24864","volume":"110","author":"Wu I.","year":"2013","journal-title":"Biotechnol. Bioeng."},{"key":"ref61\/cit61","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1093\/bioinformatics\/btg231","volume":"19","author":"Dombkowski A. A.","year":"2003","journal-title":"Bioinformatics"},{"key":"ref62\/cit62","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/1754-6834-3-20","volume":"3","author":"Lantz S. E.","year":"2010","journal-title":"Biotechnol. Biofuels"},{"key":"ref63\/cit63","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1126\/science.8036495","volume":"265","author":"Divne C.","year":"1994","journal-title":"Science"},{"key":"ref64\/cit64","doi-asserted-by":"crossref","first-page":"7745","DOI":"10.1002\/anie.200602795","volume":"45","author":"Reetz M. T.","year":"2006","journal-title":"Angew. Chem.\u2014Int. Ed."},{"key":"ref65\/cit65","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1021\/ci400044m","volume":"53","author":"Pfleger C.","year":"2013","journal-title":"J. Chem. Inf. Model."},{"key":"ref66\/cit66","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1002\/prot.22946","volume":"79","author":"Radestock S.","year":"2011","journal-title":"Proteins: Struct., Funct., Bioinf."},{"key":"ref67\/cit67","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.jbiotec.2012.01.027","volume":"159","author":"Rathi P. C.","year":"2012","journal-title":"J. Biotechnol."},{"key":"ref68\/cit68","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0263-7855(96)00018-5","volume":"14","author":"Humphrey W.","year":"1996","journal-title":"J. Mol. Graph. Model."}],"container-title":["Journal of Chemical Information and Modeling"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/ci500339v","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T02:30:01Z","timestamp":1682217001000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/ci500339v"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10,6]]},"references-count":68,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10,27]]}},"alternative-id":["10.1021\/ci500339v"],"URL":"https:\/\/doi.org\/10.1021\/ci500339v","relation":{},"ISSN":["1549-9596","1549-960X"],"issn-type":[{"type":"print","value":"1549-9596"},{"type":"electronic","value":"1549-960X"}],"subject":[],"published":{"date-parts":[[2014,10,6]]}}}