{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:18:57Z","timestamp":1788326337419,"version":"build-2803163510"},"reference-count":44,"publisher":"American Chemical Society (ACS)","issue":"5","license":[{"start":{"date-parts":[[2015,2,8]],"date-time":"2015-02-08T00:00:00Z","timestamp":1423353600000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The sensitive detection of highly toxic botulinum neurotoxin (BoNT) from Clostridium botulinum is of critical importance because it causes human illnesses if foodborne or introduced in wounds and as an iatrogenic substance. Moreover, it has been recently considered a possible biological warfare agent. Over the past decade, significant progress has been made in BoNT detection technologies, including mouse lethality assays, enzyme-linked immunosorbent assays, and endopeptidase assays and by mass spectrometry. Critical assay requirements, including rapid assay, active toxin detection, sensitive and accurate detection, still remain challenging. Here, we present a novel method to detect active BoNTs using a Glyco-quantitative polymerase chain-reaction (qPCR) approach. Sialyllactose, which interacts with the binding-domain of BoNTs, is incorporated into a sialyllactose-DNA conjugate as a binding-probe for active BoNT and recovered through BoNT-immunoprecipitation. Glyco-qPCR analysis of the bound sialyllactose-DNA is then used to detect low attomolar concentrations of BoNT and attomolar to femtomolar concentrations of BoNT in honey, the most common foodborne source of infant botulism.<\/jats:p>","DOI":"10.1021\/ac500262d","type":"journal-article","created":{"date-parts":[[2014,2,8]],"date-time":"2014-02-08T12:07:44Z","timestamp":1391861264000},"page":"2279-2284","source":"Crossref","is-referenced-by-count":6,"title":["High Sensitivity Detection of Active Botulinum Neurotoxin\nby Glyco-Quantitative Polymerase Chain-Reaction"],"prefix":"10.1021","volume":"86","author":[{"given":"Seok Joon","family":"Kwon","sequence":"first","affiliation":[{"name":"Rensselaer Polytechnic Institute , , , ,","place":["Troy, New York, United States, 12180"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Ji","family":"Jeong","sequence":"additional","affiliation":[{"name":"Konyang University , , ,","place":["Daejeon, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung Choon","family":"Yoo","sequence":"additional","affiliation":[{"name":"Konyang University , , ,","place":["Daejeon, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Cai","sequence":"additional","affiliation":[{"name":"Rensselaer Polytechnic Institute , , , ,","place":["Troy, New York, United States, 12180"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gi-Hyeok","family":"Yang","sequence":"additional","affiliation":[{"name":"Medy-Tox Inc. Microbial Toxin Research Institute, , ,","place":["Chungnam, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Chul","family":"Lee","sequence":"additional","affiliation":[{"name":"Medy-Tox Inc. Microbial Toxin Research Institute, , ,","place":["Chungnam, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan S.","family":"Dordick","sequence":"additional","affiliation":[{"name":"Rensselaer Polytechnic Institute , , , ,","place":["Troy, New York, United States, 12180"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Linhardt","sequence":"additional","affiliation":[{"name":"Rensselaer Polytechnic Institute , , , ,","place":["Troy, New York, United States, 12180"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung Bok","family":"Lee","sequence":"additional","affiliation":[{"name":"Konyang University , , ,","place":["Daejeon, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2014,2,8]]},"reference":[{"issue":"Suppl 8","key":"2026090200123748000_cit1","doi-asserted-by":"crossref","first-page":"S2","DOI":"10.1002\/mds.20003","volume":"19","author":"Erbguth","year":"2004","journal-title":"Movement Disorders"},{"key":"2026090200123748000_cit2","doi-asserted-by":"crossref","first-page":"653","DOI":"10.5858\/2004-128-653-CBATCL","volume":"128","author":"Caya","year":"2004","journal-title":"Arch. Pathol.\nLab. Med."},{"key":"2026090200123748000_cit3","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1086\/444507","volume":"41","author":"Sobel","year":"2005","journal-title":"Clin. Infect. Dis."},{"key":"2026090200123748000_cit4","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0022-3476(79)80863-X","volume":"94","author":"Arnon","year":"1979","journal-title":"J.\nPediatrics"},{"key":"2026090200123748000_cit5","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1093\/infdis\/jit449","volume":"209","author":"Barash","year":"2013","journal-title":"J. Infect. Dis."},{"key":"2026090200123748000_cit6","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1093\/infdis\/jit450","volume":"209","author":"Dover","year":"2013","journal-title":"J. Infect. 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Ed."},{"key":"2026090200123748000_cit32b","doi-asserted-by":"crossref","first-page":"2459","DOI":"10.1016\/j.bmcl.2007.02.028","volume":"17","author":"Kale","year":"2007","journal-title":"Bioorg. Med. Chem. 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