{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T09:30:35Z","timestamp":1787045435106,"version":"build-2736575974"},"reference-count":23,"publisher":"American Chemical Society (ACS)","issue":"14","license":[{"start":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:00:00Z","timestamp":1590624000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:00:00Z","timestamp":1590624000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:00:00Z","timestamp":1590624000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom?a y Competitividad","doi-asserted-by":"publisher","award":["BIO2016-80417-P"],"award-info":[{"award-number":["BIO2016-80417-P"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003451","name":"Euskal Herriko Unibertsitatea","doi-asserted-by":"publisher","award":["ESPPOC 16\/65"],"award-info":[{"award-number":["ESPPOC 16\/65"]}],"id":[{"id":"10.13039\/501100003451","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003451","name":"Euskal Herriko Unibertsitatea","doi-asserted-by":"publisher","award":["PIF16\/204"],"award-info":[{"award-number":["PIF16\/204"]}],"id":[{"id":"10.13039\/501100003451","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003451","name":"Euskal Herriko Unibertsitatea","doi-asserted-by":"publisher","award":["PIF17\/177"],"award-info":[{"award-number":["PIF17\/177"]}],"id":[{"id":"10.13039\/501100003451","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Departamento de Educaci?n del Gobierno Vasco","award":["IT127219"],"award-info":[{"award-number":["IT127219"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Low cost, easy to use cell viability tests are needed in the pharmaceutical, biomaterial, and environmental industries to measure adverse cellular effects. We present a new methodology to track cell death with high resolution. Adherent cells commonly detach from the surface when they die, but some toxic compounds promote cell adhesion. A methodology that enables both dynamic detachment monitoring but also rapid detection of toxic effects of compounds that promote cell adhesion would constitute a step forward toward high-throughput cytotoxicity measurements. We achieved dynamic digital quantification of cell viability by simple optical imaging using \u201csingle cell adhesion dot arrays\u201d (SCADA), fibronectin (FN) dot arrays designed to accommodate a single cell on each fibronectin dot. For cytotoxicity measurements, cell-filled SCADA substrates were exposed to K2CrO4, HgSO4 salts, and dimethyl sulfoxide (DMSO). The toxic effect of DMSO and K2CrO4 was dynamically monitored by measuring the cell detachment rate during more than 30 h by quantifying the number of occupied dots in the SCADA array. HgSO4 inhibited cellular detachment from the surface, and cytotoxicity was monitored using the trypan blue life\/death assay directly on the surface. In all cases, the cytotoxicity effects were easily monitored with single cell resolution, and the results were comparable to previous reports. SCADA enabled dynamic measurements at the highest resolution due to the digital measuring in this method. The integration of SCADA substrates into microfluidic platforms will provide a practical tool that will extend to fundamental research and commercial applications.<\/jats:p>","DOI":"10.1021\/acs.analchem.0c00940","type":"journal-article","created":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T03:47:13Z","timestamp":1590637633000},"page":"9658-9665","source":"Crossref","is-referenced-by-count":20,"title":["Optical Single Cell Resolution Cytotoxicity Biosensor Based on Single Cell Adhesion\nDot Arrays"],"prefix":"10.1021","volume":"92","author":[{"given":"Maite","family":"Garcia-Hernando","sequence":"first","affiliation":[{"name":"University of the Basque Country UPV\/EHU , , ,","place":["Vitoria-Gasteiz, Spain, 01006"]},{"name":"University of the Basque Country UPV\/EHU , , ,","place":["Vitoria-Gasteiz, Spain, 01006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Calatayud-Sanchez","sequence":"additional","affiliation":[{"name":"University of the Basque Country UPV\/EHU , , ,","place":["Vitoria-Gasteiz, Spain, 01006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaione","family":"Etxebarria-Elezgarai","sequence":"additional","affiliation":[{"name":"University of the Basque Country UPV\/EHU , , ,","place":["Vitoria-Gasteiz, Spain, 01006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian M.","family":"de Pancorbo","sequence":"additional","affiliation":[{"name":"University of the Basque Country UPV\/EHU , , ,","place":["Vitoria-Gasteiz, Spain, 01006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0699-5507","authenticated-orcid":true,"given":"Fernando","family":"Benito-Lopez","sequence":"additional","affiliation":[{"name":"University of the Basque Country UPV\/EHU , , ,","place":["Vitoria-Gasteiz, Spain, 01006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6638-7370","authenticated-orcid":true,"given":"Lourdes","family":"Basabe-Desmonts","sequence":"additional","affiliation":[{"name":"University of the Basque Country UPV\/EHU , , ,","place":["Vitoria-Gasteiz, Spain, 01006"]},{"name":"IKERBASQUE, Basque Foundation of Science , , ,","place":["Bilbao, Bizkaia, Spain, 48013"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,5,28]]},"reference":[{"key":"2026081803302980300_cit1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-61779-108-6","volume-title":"Mammalian Cell Viability: Methods and Protocols","author":"Stoddart","year":"2011"},{"key":"2026081803302980300_cit2","doi-asserted-by":"publisher","first-page":"1668","DOI":"10.1016\/j.celrep.2019.01.053","article-title":"Microcolony\nSize Distribution Assay Enables High-Throughput Cell Survival Quantitation","volume":"26","author":"Ngo","year":"2019","journal-title":"Cell Rep."},{"key":"2026081803302980300_cit3","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.332","article-title":"Robust high-throughput kinetic analysis\nof apoptosis\nwith real-time high-content live-cell imaging","volume":"7","author":"Gelles","year":"2016","journal-title":"Cell Death Dis."},{"key":"2026081803302980300_cit4","doi-asserted-by":"publisher","first-page":"600","DOI":"10.1016\/j.cels.2017.05.002","article-title":"Systematic\nquantification of population cell death kinetics in mammalian cells","volume":"4","author":"Forcina","year":"2017","journal-title":"Cell Syst."},{"key":"2026081803302980300_cit100","doi-asserted-by":"publisher","first-page":"3648","DOI":"10.1021\/ac2000225","article-title":"Microfluidic device with chemical\ngradient for single-cell cytotoxicity assays","volume":"83","author":"Hosokawa","year":"2011","journal-title":"Anal. 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