{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T11:16:33Z","timestamp":1792149393148,"version":"4.4.0"},"reference-count":55,"publisher":"American Chemical Society (ACS)","issue":"32","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"U.S. Department of Health and Human Services","doi-asserted-by":"publisher","award":["UH3CA255132"],"award-info":[{"award-number":["UH3CA255132"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004319","name":"Pfizer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004319","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anal. Chem."],"published-print":{"date-parts":[[2024,8,13]]},"DOI":"10.1021\/acs.analchem.4c00523","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T23:42:52Z","timestamp":1722555772000},"page":"12973-12982","source":"Crossref","is-referenced-by-count":6,"title":["Coupling Microdroplet-Based Sample Preparation, Multiplexed Isobaric Labeling, and Nanoflow Peptide Fractionation for Deep Proteome Profiling of the Tissue Microenvironment"],"prefix":"10.1021","volume":"96","author":[{"given":"Marija","family":"Veli\u010dkovi\u0107","sequence":"first","affiliation":[{"name":"Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas L.","family":"Fillmore","sequence":"additional","affiliation":[{"name":"Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9626-2069","authenticated-orcid":true,"given":"Isaac Kwame","family":"Attah","sequence":"additional","affiliation":[{"name":"Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camilo","family":"Posso","sequence":"additional","affiliation":[{"name":"Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Pino","sequence":"additional","affiliation":[{"name":"Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah M.","family":"Williams","sequence":"additional","affiliation":[{"name":"Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7945-9620","authenticated-orcid":true,"given":"Du\u0161an","family":"Veli\u010dkovi\u0107","sequence":"additional","affiliation":[{"name":"Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon M.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2722-4149","authenticated-orcid":true,"given":"Kristin E.","family":"Burnum-Johnson","sequence":"additional","affiliation":[{"name":"Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5416-0566","authenticated-orcid":true,"given":"Ying","family":"Zhu","sequence":"additional","affiliation":[{"name":"Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5108-2227","authenticated-orcid":true,"given":"Paul D.","family":"Piehowski","sequence":"additional","affiliation":[{"name":"Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2024,8,1]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.TIR118.000686"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1039\/D1AN00472G"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.2174\/138920207780076910"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201900226"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b00024"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA119.001857"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-018-1547-5"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c04174"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-01002-5"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b03349"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23667-y"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b01248"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1039\/C8SC02680G"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2022.116920"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-022-02817-5"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b00661"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26514-2"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03367-w"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2023.100665"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202100923"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b00345"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b00327"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b01921"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.057760"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201510411"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2020.00499"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b00327"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13858-z"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01551"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-018-1493-9"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00629"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2020.04.018"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2019.03.005"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1586\/epr.11.39"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c05022"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv437"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6277"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1021\/pr8001244"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1186\/s12014-022-09367-9"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100141"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1113"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201000752"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.1c00776"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2022.100426"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00042.2009"},{"key":"ref48\/cit48","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2017.08.017"},{"key":"ref49\/cit49","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-79313-y"},{"key":"ref50\/cit50","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c200026"},{"key":"ref51\/cit51","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201604030"},{"key":"ref52\/cit52","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2014.03.011"},{"key":"ref53\/cit53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115421"},{"key":"ref54\/cit54","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260419"},{"key":"ref55\/cit55","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2023.100592"}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.4c00523","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.4c00523","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T08:15:35Z","timestamp":1723536935000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.4c00523"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,1]]},"references-count":55,"journal-issue":{"issue":"32","published-print":{"date-parts":[[2024,8,13]]}},"alternative-id":["10.1021\/acs.analchem.4c00523"],"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.4c00523","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,1]]}}}