{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T12:00:15Z","timestamp":1794312015466,"version":"4.9.0"},"reference-count":47,"publisher":"American Chemical Society (ACS)","issue":"6","license":[{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22204072"],"award-info":[{"award-number":["22204072"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172691"],"award-info":[{"award-number":["82172691"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82473307"],"award-info":[{"award-number":["82473307"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Jiangsu Provincial Social Development Project","award":["BE2023659"],"award-info":[{"award-number":["BE2023659"]}]},{"name":"Jiangsu Province Capability Improvement Project through Science,Technology and Education,Jiangsu Provincial Medical Key Discipline (Laboratory) Cultivation Unit","award":["JSDW202221"],"award-info":[{"award-number":["JSDW202221"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anal. Chem."],"published-print":{"date-parts":[[2025,2,18]]},"DOI":"10.1021\/acs.analchem.4c05648","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T15:48:41Z","timestamp":1738770521000},"page":"3438-3448","source":"Crossref","is-referenced-by-count":4,"title":["In Vivo Time-Resolved Single-Cell RNA-Seq Reveals Chemotherapy-Induced Transcriptional Dynamics in Tumor Infiltrating Lymphocytes"],"prefix":"10.1021","volume":"97","author":[{"given":"Wenqi","family":"Zhu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center at Nanjing University, School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Avenue, Nanjing, Jiangsu 210008, China"},{"name":"Singleron Biotechnologies","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8004-9976","authenticated-orcid":true,"given":"Xiangyu","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Urology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School","place":["Nanjing, China"]},{"name":"Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3391-1041","authenticated-orcid":true,"given":"Zihan","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center at Nanjing University, School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Avenue, Nanjing, Jiangsu 210008, China"},{"name":"Department of Urology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School","place":["Nanjing, China"]},{"name":"Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Urology","place":["Nanjing, China"]},{"name":"Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese Medicine","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center at Nanjing University, School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Avenue, Nanjing, Jiangsu 210008, China"},{"name":"University of Glasgow, University Avenue, Glasgow G12 8QQ, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek","family":"Zhao","sequence":"additional","affiliation":[{"name":"Lake Oswego High School, 2501 Country Club Road, Lake Oswego, Oregon 97034, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Urology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School","place":["Nanjing, China"]},{"name":"Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School","place":["Nanjing, China"]},{"name":"Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3121-5157","authenticated-orcid":true,"given":"Hongqian","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Urology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School","place":["Nanjing, China"]},{"name":"Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Fang","sequence":"additional","affiliation":[{"name":"Singleron Biotechnologies","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8065-5749","authenticated-orcid":true,"given":"Jie P.","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center at Nanjing University, School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Avenue, Nanjing, Jiangsu 210008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School","place":["Nanjing, China"]},{"name":"Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5712-6162","authenticated-orcid":true,"given":"Tian","family":"Tian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center at Nanjing University, School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Avenue, Nanjing, Jiangsu 210008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2025,2,5]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-023-00615-w"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c05785"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.20892\/j.issn.2095-3941.2024.0012"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-120123-105748"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-020-0668-4"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-018-0288-5"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1039\/D1LC00607J"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-022-00157-z"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2859"},{"issue":"7","key":"ref10\/cit10","first-page":"e54","volume":"45","author":"Guo M.","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0414-6"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01622-1"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1654-9"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-023-01257-5"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-021-00444-7"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.12.029"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.11.032"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1184-5"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-023-01899-4"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1369-y"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-0935-4"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36902-5"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1186\/s40364-024-00643-4"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4282"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c02602"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.4c00635"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1039\/D0SC00197J"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1039\/D2CB00046F"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1607303"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2009.07.030"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2016.08.002"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c02765"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b00554"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2167"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.107357"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22147512"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-020-00308-y"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2024.04.032"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-024-01221-7"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1085476"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39920-5"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2016.01.017"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202317613"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)32598-8"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-1298"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2024.04.032"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-54202-4"}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.4c05648","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.4c05648","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T04:13:03Z","timestamp":1739851983000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.4c05648"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,5]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,2,18]]}},"alternative-id":["10.1021\/acs.analchem.4c05648"],"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.4c05648","relation":{"has-part":[{"id-type":"doi","id":"https:\/\/doi.org\/10.1021\/acs.analchem.4c05648.s001","asserted-by":"subject"}]},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,5]]}}}