{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T09:20:38Z","timestamp":1762852838757,"version":"build-2065373602"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"44","license":[{"start":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:00:00Z","timestamp":1760313600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:00:00Z","timestamp":1760313600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:00:00Z","timestamp":1760313600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100004040","name":"KU Leuven","doi-asserted-by":"publisher","award":["C24E\/20\/035","C3\/19\/024","iBOF\/23\/005","KA\/20\/019"],"award-info":[{"award-number":["C24E\/20\/035","C3\/19\/024","iBOF\/23\/005","KA\/20\/019"]}],"id":[{"id":"10.13039\/501100004040","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["1S43421N","G090120N","S000825N"],"award-info":[{"award-number":["1S43421N","G090120N","S000825N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anal. Chem."],"published-print":{"date-parts":[[2025,11,11]]},"DOI":"10.1021\/acs.analchem.5c03396","type":"journal-article","created":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:46:38Z","timestamp":1760366798000},"page":"24359-24369","source":"Crossref","is-referenced-by-count":0,"title":["Multiplex and Spatiotemporal Detection of Single-Cell Antibody Secretions Using Protein-Patterned Microwells"],"prefix":"10.1021","volume":"97","author":[{"given":"Julie","family":"Van Lent","sequence":"first","affiliation":[{"name":"Department of Biosystems, Biosensors Group","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]},{"name":"LISCO, the KU Leuven Institute for Single Cell Omics","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5906-0532","authenticated-orcid":true,"given":"Karen","family":"Ven","sequence":"additional","affiliation":[{"name":"Department of Biosystems, Biosensors Group","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]},{"name":"LISCO, the KU Leuven Institute for Single Cell Omics","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maya","family":"Imbrechts","sequence":"additional","affiliation":[{"name":"Laboratory for Therapeutic and Diagnostic Antibodies","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]},{"name":"PharmAbs, The KU Leuven Antibody Center","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nick","family":"Geukens","sequence":"additional","affiliation":[{"name":"PharmAbs, The KU Leuven Antibody Center","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1259-9105","authenticated-orcid":true,"given":"Paul","family":"Declerck","sequence":"additional","affiliation":[{"name":"Laboratory for Therapeutic and Diagnostic Antibodies","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]},{"name":"PharmAbs, The KU Leuven Antibody Center","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Vanhoorelbeke","sequence":"additional","affiliation":[{"name":"PharmAbs, The KU Leuven Antibody Center","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]},{"name":"Laboratory for Thrombosis Research","place":["Kortrijk, Belgium"]},{"name":"IRF Life Sciences, KU Leuven Campus Kulak Kortrijk","place":["Kortrijk, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8143-6794","authenticated-orcid":true,"given":"Jeroen","family":"Lammertyn","sequence":"additional","affiliation":[{"name":"Department of Biosystems, Biosensors Group","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]},{"name":"LISCO, the KU Leuven Institute for Single Cell Omics","place":["Leuven, Belgium"]},{"name":"KU Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"316","published-online":{"date-parts":[[2025,10,13]]},"reference":[{"key":"ref1\/cit1","volume-title":"Mol. Biol. Cell","author":"Alberts B.","year":"2002","edition":"4"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1093\/ilar.46.3.258"},{"key":"ref3\/cit3","doi-asserted-by":"crossref","unstructured":"Philippidis, A., Top 10 Best Selling Drugs,\n                      Genetic Engineering & Biotechnology News\n                      , 2024. [Online]. Available: https:\/\/www.genengnews.com\/topics\/drug-discovery\/top-10-best-selling-drugs\/ (accessed Aug 23 2024).","DOI":"10.1089\/gen.44.07.07"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.106639"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1186\/s43141-021-00264-6"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-019-0551-y"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9853-1_23"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8958-4_5"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(89)90150-6"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1039\/D1LC00243K"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1002\/biot.202100623"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1039\/D3LC00887H"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1007\/s40259-024-00667-0"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0466-7"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1177\/2472630319892571"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1204514109"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-023-01017-1"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1021\/ac101956e"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1039\/c0lc00150c"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1089\/gen.40.02.09"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8958-4_6"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1210"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2019.1624126"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202048976"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201801361"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.109840"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1039\/C5AN01648G"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201600725"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-01841-y"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202201477"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1039\/D4LC00904E"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-anchem-061318-115055"},{"key":"ref33\/cit33","unstructured":"Burgoyne, F., Optimal protocol for moulding PDMS with a PDMS master \u2013 Chips and Tips. 2010. [Online]. Available: https:\/\/blogs.rsc.org\/chipsandtips\/2010\/07\/06\/optimal-protocol-for-moulding-pdms-with-a-pdms-master\/?doing_wp_cron=1721396018.8917169570922851562500 (accessed Aug 23 2024)."},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201504154"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000340"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260360814"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.107574"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104515"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260341109"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1966"},{"key":"ref42\/cit42","unstructured":"Single cell analysis \u2013 VyCAP [Online]. Available: https:\/\/www.vycap.com\/technology\/isolate-single-cells\/ (accessed Aug 19 2025)."},{"key":"ref43\/cit43","unstructured":"Antibody discovery\nand cell line generation \u2013 VyCAP [Online].\nAvailable: https:\/\/www.vycap.com\/applications\/antibody-discovery-and-cell-line-generation\/ (accessed Aug 19 2025)."},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1039\/C9LC00100J"},{"key":"ref45\/cit45","unstructured":"Aridis Pharmaceuticals | \u03bbPEXTM [Online]. Available: https:\/\/www.aridispharma.com\/\u03bbpex\/ (accessed Aug 25 2025)."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.5c03396","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.5c03396","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T09:15:26Z","timestamp":1762852526000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.5c03396"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,13]]},"references-count":45,"journal-issue":{"issue":"44","published-print":{"date-parts":[[2025,11,11]]}},"alternative-id":["10.1021\/acs.analchem.5c03396"],"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.5c03396","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"type":"print","value":"0003-2700"},{"type":"electronic","value":"1520-6882"}],"subject":[],"published":{"date-parts":[[2025,10,13]]}}}