{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T09:36:26Z","timestamp":1793266586623,"version":"4.5.0"},"reference-count":47,"publisher":"American Chemical Society (ACS)","issue":"44","license":[{"start":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T00:00:00Z","timestamp":1793232000000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai Municipality","doi-asserted-by":"publisher","award":["23dz2260100"],"award-info":[{"award-number":["23dz2260100"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62425501"],"award-info":[{"award-number":["62425501"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["17YF1402100"],"award-info":[{"award-number":["17YF1402100"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010108","name":"Zhongshan Hospital","doi-asserted-by":"publisher","award":["2019ZSYQ05"],"award-info":[{"award-number":["2019ZSYQ05"]}],"id":[{"id":"10.13039\/501100010108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFF0502900"],"award-info":[{"award-number":["2021YFF0502900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Congenital defects and abnormal hemodynamics are widely accepted theories in bicuspid aortic valve (BAV)-associated aortopathy. However, current clinical criteria for surgical intervention in BAV patients fall short of direct correlation with neither factor. Here, we applied nonlinear optical (NLO) microscopy to investigate the microstructural changes of the aortic wall associated with bicuspid aortopathy at different stages. Fresh aortic specimens were collected from 24 study patients and assessed by NLO scanning layer-by-layer to probe the distribution of the extracellular matrix and smooth muscle cells (SMCs). NLO images demonstrated near-perfect concordance with traditional histology (Masson\u2019s and Weigert\u2019s staining), affirming its reliability for histologic assessment. In BAV-associated aortic dilatation (BAV-AD), collagen fibers exhibited better alignment with a helix-like pattern compared to tricuspid aortic valve (TAV) patients, while their content was markedly reduced relative to nondilated BAV (BAV-NA) cases. Notably, both collagen and elastin fibers in BAV patients displayed more ordered alignment in regions subjected to high wall shear stress than in low wall shear stress regions. Quantitative analysis of fiber anisotropy and relative abundance revealed distinct interpatient and spatial variations in collagen organization, underscoring the complex biomechanical landscape associated with aortic remodeling. Our results demonstrate that rapid and precise tomography of aortic architecture could be realized by NLO microscopy, serving as a potential histological marker to guide surgical intervention in bicuspid aortopathy.<\/jats:p>","DOI":"10.1021\/acs.analchem.5c04657","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T02:45:30Z","timestamp":1761792330000},"page":"24596-24607","source":"Crossref","is-referenced-by-count":0,"title":["Shear Stress-Induced\nMicrostructure Remodeling of\nHuman Bicuspid Aortopathy Revealed by Nonlinear Optical Microscopy"],"prefix":"10.1021","volume":"97","author":[{"given":"Lili","family":"Zhang","sequence":"first","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]},{"name":"Shanghai Jiao Tong University School of Medicine , , ,","place":["Shanghai, China, 200025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuntao","family":"Lu","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200030"]},{"name":"Shanghai Engineering Research Center of Cardiac Valve , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wencong","family":"Hong","sequence":"additional","affiliation":[{"name":"The Hospital of Nanan City , , ,","place":["Nanan, China, 362300"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Cong","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200030"]},{"name":"Shanghai Engineering Research Center of Cardiac Valve , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaying","family":"Li","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Liu","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200030"]},{"name":"Shanghai Engineering Research Center of Cardiac Valve , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunsheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200030"]},{"name":"Shanghai Engineering Research Center of Cardiac Valve , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lai","family":"Wei","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200030"]},{"name":"Shanghai Engineering Research Center of Cardiac Valve , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianpeng","family":"Ao","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenshuo","family":"Wang","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200030"]},{"name":"Shanghai Engineering Research Center of Cardiac Valve , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9066-4008","authenticated-orcid":true,"given":"Minbiao","family":"Ji","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"issue":"8","key":"2026102905064716600_cit1","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1016\/j.amjmed.2016.03.009","article-title":"Bicuspid\nAortic Valve: Unlocking the Morphogenetic Puzzle","volume":"129","author":"Longobardo","year":"2016","journal-title":"Am. J. Med."},{"key":"2026102905064716600_cit2","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1016\/j.ijcard.2015.08.106","article-title":"Bicuspid aortic\nvalve aortopathy in adults: Incidence,\netiology, and clinical significance","volume":"201","author":"Michelena","year":"2015","journal-title":"Int. J.\nCardiol."},{"issue":"25","key":"2026102905064716600_cit3","doi-asserted-by":"publisher","first-page":"2691","DOI":"10.1161\/circulationaha.113.007851","article-title":"Bicuspid Aortic Valve","volume":"129","author":"Michelena","year":"2014","journal-title":"Circulation"},{"issue":"6","key":"2026102905064716600_cit4","doi-asserted-by":"publisher","first-page":"724","DOI":"10.1016\/j.jacc.2015.11.006","article-title":"Surgery for Aortic\nDilatation in Patients With Bicuspid Aortic Valves","volume":"67","author":"Hiratzka","year":"2016","journal-title":"J. Am. Coll. Cardiol."},{"issue":"36","key":"2026102905064716600_cit5","doi-asserted-by":"publisher","first-page":"2739","DOI":"10.1093\/eurheartj\/ehx391","article-title":"ESC\/EACTS guidelines for the management\nof valvular heart disease","volume":"38","author":"Baumgartner","year":"2017","journal-title":"Eur. Heart J."},{"issue":"2","key":"2026102905064716600_cit6","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/j.jtcvs.2017.10.161","article-title":"The American Association for Thoracic Surgery consensus\nguidelines on bicuspid aortic valve\u2013related aortopathy: Executive\nsummary","volume":"156","author":"Borger","year":"2018","journal-title":"J. Thorac. Cardiovasc. Surg."},{"issue":"5909","key":"2026102905064716600_cit7","doi-asserted-by":"publisher","first-page":"1857","DOI":"10.1126\/science.1165758","article-title":"Label-Free Biomedical\nImaging with High Sensitivity by Stimulated Raman Scattering Microscopy","volume":"322","author":"Freudiger","year":"2008","journal-title":"Science"},{"issue":"4951","key":"2026102905064716600_cit8","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1126\/science.2321027","article-title":"Two-photon laser scanning fluorescence\nmicroscopy","volume":"248","author":"Denk","year":"1990","journal-title":"Science"},{"issue":"7314","key":"2026102905064716600_cit9","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1038\/467407a","article-title":"Biological\nimaging: Beyond fluorescence","volume":"467","author":"Cohen","year":"2010","journal-title":"Nature"},{"issue":"20","key":"2026102905064716600_cit10","doi-asserted-by":"publisher","first-page":"7907","DOI":"10.1021\/acs.analchem.4c02061","article-title":"Stain-Free Histopathology with Stimulated\nRaman Scattering Microscopy","volume":"96","author":"Ma","year":"2024","journal-title":"Anal. Chem."},{"issue":"10","key":"2026102905064716600_cit11","doi-asserted-by":"publisher","first-page":"105401","DOI":"10.1088\/1612-202x\/aa7c9a","article-title":"Label-free imaging of\nbrain and brain tumor specimens with combined\ntwo-photon excited fluorescence and second harmonic generation microscopy","volume":"14","author":"Jiang","year":"2017","journal-title":"Laser Phys. Lett."},{"issue":"5","key":"2026102905064716600_cit12","doi-asserted-by":"publisher","first-page":"1730010","DOI":"10.1142\/S1793545817300105","article-title":"Stimulated Raman scattering microscopy\nfor rapid brain tumor histology","volume":"10","author":"Yang","year":"2017","journal-title":"J. Innovative\nOpt. Health Sci."},{"issue":"15","key":"2026102905064716600_cit13","doi-asserted-by":"publisher","first-page":"4129","DOI":"10.7150\/thno.26946","article-title":"Label-free\nimaging of hemoglobin\ndegradation and hemosiderin formation in brain tissues with femtosecond\npump-probe microscopy","volume":"8","author":"Zhang","year":"2018","journal-title":"Theranostics"},{"key":"2026102905064716600_cit14","doi-asserted-by":"crossref","unstructured":"Eibl, M.; Karpf, S.; Hakert, H.; Weng, D.; Huber, R.. In  Two-photon-excited fluorescence\n(TPEF) and fluorescence\nlifetime imaging (FLIM) with sub-nanosecond pulses and a high analog\nbandwidth signal detection; SPIE, 2017.","DOI":"10.1117\/12.2250831"},{"issue":"7","key":"2026102905064716600_cit15","doi-asserted-by":"publisher","first-page":"1813","DOI":"10.1016\/j.optcom.2007.07.067","article-title":"Label-free\nImaging of Arterial Cells\nand Extracellular Matrix Using a Multimodal CARS Microscope","volume":"281","author":"Wang","year":"2008","journal-title":"Opt. Commun."},{"issue":"4","key":"2026102905064716600_cit16","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1038\/nprot.2012.009","article-title":"Second\nharmonic generation microscopy for quantitative analysis of collagen\nfibrillar structure","volume":"7","author":"Chen","year":"2012","journal-title":"Nat. Protoc."},{"issue":"6","key":"2026102905064716600_cit17","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1002\/jbio.200810060","article-title":"Three-dimensional (3D)\nbackward and forward second\nharmonic generation (SHG) microscopy of biological tissues","volume":"1","author":"Bianchini","year":"2008","journal-title":"J. Biophotonics"},{"issue":"2","key":"2026102905064716600_cit18","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1364\/OL.11.000094","article-title":"Second-harmonic microscopy of biological tissue","volume":"11","author":"Freund","year":"1986","journal-title":"Opt. 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Commun."},{"issue":"46","key":"2026102905064716600_cit22","doi-asserted-by":"publisher","first-page":"15550","DOI":"10.1021\/acs.analchem.1c03861","article-title":"Label-Free Histology and Evaluation of Human\nPancreatic Cancer with\nCoherent Nonlinear Optical Microscopy","volume":"93","author":"Zhang","year":"2021","journal-title":"Anal.\nChem."},{"issue":"4","key":"2026102905064716600_cit23","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1158\/0008-5472.CAN-22-2146","article-title":"Stimulated Raman Scattering\nMicroscopy Enables Gleason Scoring of Prostate Core Needle Biopsy\nby a Convolutional Neural Network","volume":"83","author":"Ao","year":"2023","journal-title":"Cancer Res."},{"issue":"2","key":"2026102905064716600_cit24","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201700005","article-title":"Chemical imaging\nof fresh vascular smooth muscle cell\nresponse by epi-detected stimulated Raman scattering","volume":"11","author":"Zhang","year":"2018","journal-title":"J. Biophotonics"},{"issue":"9","key":"2026102905064716600_cit25","doi-asserted-by":"publisher","first-page":"2541","DOI":"10.7150\/thno.32655","article-title":"Rapid\nhistology of laryngeal squamous cell carcinoma with deep-learning\nbased stimulated Raman scattering microscopy","volume":"9","author":"Zhang","year":"2019","journal-title":"Theranostics"},{"issue":"309","key":"2026102905064716600_cit26","doi-asserted-by":"publisher","first-page":"309ra163","DOI":"10.1126\/scitranslmed.aab0195","article-title":"Detection\nof human brain tumor infiltration with quantitative stimulated Raman\nscattering microscopy","volume":"7","author":"Ji","year":"2015","journal-title":"Sci. Transl. Med."},{"issue":"40","key":"2026102905064716600_cit27","doi-asserted-by":"publisher","first-page":"405401","DOI":"10.1088\/0022-3727\/48\/40\/405401","article-title":"Identifying three different\narchitectural subtypes of mammary ductal\ncarcinoma in situ using multiphoton microscopy","volume":"48","author":"Wu","year":"2015","journal-title":"J. Phys. D: Appl. Phys."},{"issue":"11","key":"2026102905064716600_cit28","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aat7715","article-title":"Label-free imaging\nof amyloid plaques in Alzheimer\u2019s\ndisease with stimulated Raman scattering microscopy","volume":"4","author":"Ji","year":"2018","journal-title":"Sci. Adv."},{"issue":"6","key":"2026102905064716600_cit29","doi-asserted-by":"publisher","first-page":"2604","DOI":"10.1364\/boe.9.002604","article-title":"Rapid large-scale stimulated Raman histology with strip\nmosaicing and dual-phase detection","volume":"9","author":"Zhang","year":"2018","journal-title":"Biomed.\nOpt. Express"},{"issue":"1","key":"2026102905064716600_cit30","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1364\/OPTICA.4.000044","article-title":"Dual-phase stimulated Raman scattering microscopy for real-time two-color\nimaging","volume":"4","author":"He","year":"2017","journal-title":"Optica"},{"issue":"4","key":"2026102905064716600_cit31","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1364\/OL.42.000659","article-title":"Stimulated\nRaman scattering microscopy and spectroscopy with a rapid scanning\noptical delay line","volume":"42","author":"He","year":"2017","journal-title":"Opt. Lett."},{"issue":"7","key":"2026102905064716600_cit32","doi-asserted-by":"publisher","first-page":"3074","DOI":"10.7150\/thno.53755","article-title":"Highly specific and label-free histological\nidentification of microcrystals in fresh human gout tissues with stimulated\nRaman scattering","volume":"11","author":"Zhang","year":"2021","journal-title":"Theranostics"},{"issue":"5","key":"2026102905064716600_cit33","doi-asserted-by":"publisher","first-page":"1507","DOI":"10.1007\/s10237-019-01161-1","article-title":"A computational\nmodel for understanding\nthe micro-mechanics of collagen fiber network in the tunica adventitia","volume":"18","author":"Ayyalasomayajula","year":"2019","journal-title":"Biomech. Model. Mechanobiol."},{"issue":"6","key":"2026102905064716600_cit34","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1016\/j.ejcts.2011.01.001","article-title":"Is aortopathy in bicuspid aortic valve disease a congenital\ndefect or a result of abnormal hemodynamics? A critical reappraisal\nof a one-sided argument","volume":"39","author":"Girdauskas","year":"2011","journal-title":"Eur. J. Cardio. Thorac.\nSurg."},{"issue":"8","key":"2026102905064716600_cit35","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1016\/j.jacc.2015.06.1310","article-title":"Valve-Related\nHemodynamics\nMediate Human Bicuspid Aortopathy: Insights From Wall Shear Stress\nMapping","volume":"66","author":"Guzzardi","year":"2015","journal-title":"J. Am. Coll. Cardiol."},{"issue":"6","key":"2026102905064716600_cit36","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1161\/CIRCULATIONAHA.113.003026","article-title":"Bicuspid\naortic cusp fusion morphology alters aortic\nthree-dimensional outflow patterns, wall shear stress, and expression\nof aortopathy","volume":"129","author":"Mahadevia","year":"2014","journal-title":"Circulation"},{"issue":"5","key":"2026102905064716600_cit37","doi-asserted-by":"publisher","first-page":"1226","DOI":"10.1016\/j.jtcvs.2007.01.039","article-title":"A classification\nsystem for the bicuspid aortic valve\nfrom 304 surgical specimens","volume":"133","author":"Sievers","year":"2007","journal-title":"J. Thorac. Cardiovasc.\nSurg."},{"issue":"15","key":"2026102905064716600_cit38","doi-asserted-by":"publisher","first-page":"1786","DOI":"10.1016\/j.jacc.2016.02.015","article-title":"Functional Aortic Root Parameters\nand Expression of Aortopathy in Bicuspid Versus Tricuspid Aortic Valve\nStenosis","volume":"67","author":"Girdauskas","year":"2016","journal-title":"J. Am. Coll. Cardiol."},{"issue":"1","key":"2026102905064716600_cit39","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1186\/1532-429X-14-84","article-title":"Evaluation\nof 3D blood flow patterns and wall shear stress in the normal and\ndilated thoracic aorta using flow-sensitive 4D CMR","volume":"14","author":"Bu\u0308rk","year":"2012","journal-title":"J. Cardiovasc. Magn. Reson."},{"issue":"3","key":"2026102905064716600_cit40","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1093\/ejcts\/ezw123","article-title":"Natural history of 40\u201350 mm root\/ascending aortic\naneurysms in the current era of dedicated thoracic aortic clinics","volume":"50","author":"Gagne\u0301-Loranger","year":"2016","journal-title":"Eur. J. Cardio. Thorac. Surg."},{"issue":"25","key":"2026102905064716600_cit41","doi-asserted-by":"publisher","first-page":"2691","DOI":"10.1161\/circulationaha.113.007851","article-title":"Bicuspid Aortic Valve","volume":"129","author":"Michelena","year":"2014","journal-title":"Circulation"},{"issue":"2","key":"2026102905064716600_cit42","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1136\/heartjnl-2013-304920","article-title":"Aortic\ndilatation patterns and rates in adults with\nbicuspid aortic valves: a comparative study with Marfan syndrome and\ndegenerative aortopathy","volume":"100","author":"Detaint","year":"2014","journal-title":"Heart"},{"issue":"15","key":"2026102905064716600_cit43","doi-asserted-by":"publisher","first-page":"6223","DOI":"10.1021\/acs.analchem.1c00522","article-title":"Microcalcification-Based\nTumor Malignancy\nEvaluation in Fresh Breast Biopsies with Hyperspectral Stimulated\nRaman Scattering","volume":"93","author":"Yang","year":"2021","journal-title":"Anal. Chem."},{"issue":"14","key":"2026102905064716600_cit44","doi-asserted-by":"publisher","first-page":"6608","DOI":"10.1073\/pnas.1813044116","article-title":"Volumetric chemical imaging by clearing-enhanced stimulated\nRaman scattering microscopy","volume":"116","author":"Wei","year":"2019","journal-title":"Proc. Natl. Acad.\nSci. U.S.A."},{"issue":"3","key":"2026102905064716600_cit45","doi-asserted-by":"publisher","first-page":"1056","DOI":"10.1016\/j.jtcvs.2013.04.028","article-title":"Mechanism of aortic medial matrix remodeling is distinct\nin patients with bicuspid aortic valve","volume":"147","author":"Phillippi","year":"2014","journal-title":"J. Thorac.\nCardiovasc. Surg."},{"issue":"6","key":"2026102905064716600_cit46","doi-asserted-by":"publisher","first-page":"1718","DOI":"10.1016\/j.jtcvs.2016.02.019","article-title":"Extracellular matrix fiber microarchitecture\nis region-specific\nin bicuspid aortic valve-associated ascending aortopathy","volume":"151","author":"Tsamis","year":"2016","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"2026102905064716600_cit47","volume-title":"Histology: A Text and\nAtlas: With Correlated Cell and Molecular Biology","author":"Ross","year":"2011"}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.5c04657","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/97\/44\/24596\/69020246\/acs.analchem.5c04657.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/97\/44\/24596\/69020246\/acs.analchem.5c04657.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T09:11:02Z","timestamp":1793265062000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/97\/44\/24596\/3624119\/Shear-Stress-Induced-Microstructure-Remodeling-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,29]]},"references-count":47,"journal-issue":{"issue":"44","published-online":{"date-parts":[[2025,10,29]]},"published-print":{"date-parts":[[2025,11,11]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.5c04657","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,29]]}}}