{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T18:57:02Z","timestamp":1793818622674,"version":"4.6.1"},"reference-count":90,"publisher":"American Chemical Society (ACS)","issue":"32","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000002","name":"US National Institutes of Health","doi-asserted-by":"crossref","award":["R01AI155735"],"award-info":[{"award-number":["R01AI155735"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University of Florida, USA"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mosquito-borne viruses pose a significant global health challenge, particularly in resource-limited settings where multiple viruses often cause illnesses with similar symptoms that require different treatment. We introduce the first 7-plex reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in a hand-held device capable of detecting the presence of Chikungunya virus (CHIKV), dengue virus serotypes (DENV 1\u20134), Mayaro virus (MAYV), and Zika virus (ZIKV) in a single test. If the result is positive from the single-plex device for the 7-plex assay, 3-plex and 4-plex devices are then used to identify the exact virus within a specimen. In-situ detection is achieved by integrating valve-enabled, paper-based sample preparation with fluorescence detection using a blue LED flashlight as a light source and a yellow plastic film as a filter, allowing visual discrimination between positive and negative samples by the naked eye or by recording images using a smart phone. The detection limits ranged from 2 genome equivalents (GE)\/reaction (for ZIKV) to 92 GE\/reaction (for DENV-3) across 7 types of viruses when 1 \u03bcL of viral RNA was used. We observed 90% overall agreement between the point-of-care (POC) device and lab-based reverse transcription polymerase chain reactions (RT-PCR) when blinded clinical specimens were tested. This assay and device have a potential to address critical surveillance gaps in endemic regions, enabling timely detection of multiple mosquito-borne viruses to guide appropriate clinical management and public health countermeasures in settings where laboratory resources are scarce.<\/jats:p>","DOI":"10.1021\/acs.analchem.5c08263","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:02:49Z","timestamp":1785934969000},"page":"23450-23463","source":"Crossref","is-referenced-by-count":2,"title":["Multiplexed\nRT-LAMP Assays in Handheld Devices for\nIn-Situ Detection of Chikungunya, Dengue, Mayaro, and Zika Viruses"],"prefix":"10.1021","volume":"98","author":[{"given":"George","family":"Adedokun","sequence":"first","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morteza","family":"Alipanah","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracey L.","family":"Moquin","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32610"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca L.","family":"Rollins","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rigan","family":"Louis","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32610"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"J. Glenn","family":"Morris","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32610"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Lednicky","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32610"]},{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32610"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1812-8016","authenticated-orcid":true,"given":"Z. Hugh","family":"Fan","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]},{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"issue":"9","key":"2026081809072635300_cit1","doi-asserted-by":"publisher","first-page":"1508","DOI":"10.1038\/s41564-019-0476-8","article-title":"The current\nand future global distribution and population at risk of dengue","volume":"4","author":"Messina","year":"2019","journal-title":"Nat. Microbiol"},{"issue":"13","key":"2026081809072635300_cit2","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1056\/NEJMra1406035","article-title":"Chikungunya virus and\nthe global spread of a mosquito-borne\ndisease","volume":"372","author":"Weaver","year":"2015","journal-title":"N Engl J. Med."},{"issue":"3","key":"2026081809072635300_cit3","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1128\/CMR.00072-15","article-title":"Zika Virus","volume":"29","author":"Musso","year":"2016","journal-title":"Clin. Microbiol.\nRev."},{"issue":"3","key":"2026081809072635300_cit4","doi-asserted-by":"publisher","first-page":"e101","DOI":"10.1016\/S1473-3099(16)30518-7","article-title":"Epidemic arboviral diseases: priorities for research\nand public health","volume":"17","author":"Wilder-Smith","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"2026081809072635300_cit5","unstructured":"World Health Organization.\n          \n          Global Arbovirus\nInitiative. Available via the Internet at: https:\/\/www.who.int\/initiatives\/global-arbovirus-initiative (accessed May 19, 2026)."},{"key":"2026081809072635300_cit6","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/978-3-031-68419-7_5","article-title":"Mayaro Virus in the Americas:\nA Short Review","volume-title":"Emerging Viruses in Latin\nAmerica: Contemporary Virology","author":"Lednicky","year":"2024"},{"issue":"7446","key":"2026081809072635300_cit7","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution\nand burden\nof dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"2026081809072635300_cit8","unstructured":"World Health Organization. Dengue and severe\ndengue. Available via the Internet at: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/dengue-and-severe-dengue (accessed May 19, 2026)."},{"key":"2026081809072635300_cit9","doi-asserted-by":"publisher","first-page":"S7","DOI":"10.1038\/nrmicro2460","article-title":"Dengue:\na continuing global threat","volume":"8","author":"Guzman","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"issue":"2","key":"2026081809072635300_cit10","doi-asserted-by":"publisher","first-page":"245","DOI":"10.3390\/pathogens12020245","article-title":"Emerging and Re-Emerging Diseases\nCaused by Badnaviruses","volume":"12","author":"Ishwara Bhat","year":"2023","journal-title":"Pathogens"},{"key":"2026081809072635300_cit11","unstructured":"U.S. Centers for Disease Control and Prevention.\n          , Surveillance\nguide for reporting arboviral diseases: Human case data only. Available via the Internet at: https:\/\/ndc.services.cdc.gov\/wp-content\/uploads\/ArboviralDiseaseReportingSurveillanceGuide_December-2021_12-10-21.pdf (accessed May 18, 2026)."},{"issue":"21","key":"2026081809072635300_cit12","doi-asserted-by":"publisher","first-page":"13458","DOI":"10.1021\/acs.analchem.9b02455","article-title":"Selective\nDetermination of Isothermally Amplified Zika Virus RNA Using a Universal\nDNA-Hairpin Probe in Less than 1 h","volume":"91","author":"Lynch","year":"2019","journal-title":"Analytical\nchemistry"},{"issue":"9","key":"2026081809072635300_cit13","doi-asserted-by":"publisher","first-page":"1207","DOI":"10.1016\/j.cmi.2021.03.015","article-title":"The role of antibody indexes in clinical\nvirology","volume":"27","author":"Shamier","year":"2021","journal-title":"Clin Microbiol Infect"},{"issue":"1","key":"2026081809072635300_cit14","doi-asserted-by":"publisher","first-page":"22323","DOI":"10.1038\/s41598-022-27028-7","article-title":"Molecular\nsurveillance of arboviruses circulation and co-infection during a\nlarge chikungunya virus outbreak in Thailand, October 2018 to February\n2020","volume":"12","author":"Khongwichit","year":"2022","journal-title":"Sci. Rep."},{"issue":"5","key":"2026081809072635300_cit15","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.3390\/microorganisms11051159","article-title":"Latest\nAdvances in Arbovirus Diagnostics","volume":"11","author":"Varghese","year":"2023","journal-title":"Microorganisms"},{"issue":"5","key":"2026081809072635300_cit16","doi-asserted-by":"publisher","first-page":"302","DOI":"10.3390\/diagnostics10050302","article-title":"Serological Diagnosis\nof Flavivirus-Associated Human\nInfections","volume":"10","author":"Musso","year":"2020","journal-title":"Diagnostics (Basel)"},{"issue":"8","key":"2026081809072635300_cit17","doi-asserted-by":"publisher","first-page":"5415","DOI":"10.1021\/acs.analchem.9b00704","article-title":"Rapid Diagnostic Platform\nfor Colorimetric\nDifferential Detection of Dengue and Chikungunya Viral Infections","volume":"91","author":"Wang","year":"2019","journal-title":"Anal. Chem."},{"issue":"1","key":"2026081809072635300_cit18","doi-asserted-by":"publisher","first-page":"18","DOI":"10.3390\/antib12010018","article-title":"Serological Cross-Reactivity in Zoonotic\nFlaviviral Infections of Medical Importance","volume":"12","author":"Gomes da Silva","year":"2023","journal-title":"Antibodies (Basel)"},{"issue":"37","key":"2026081809072635300_cit19","doi-asserted-by":"publisher","first-page":"22727","DOI":"10.1073\/pnas.2014739117","article-title":"Rapid isothermal amplification and\nportable detection system for SARS-CoV-2","volume":"117","author":"Ganguli","year":"2020","journal-title":"Proc.\nNatl. Acad. Sci. U.S.A."},{"issue":"15","key":"2026081809072635300_cit20","doi-asserted-by":"publisher","first-page":"3626","DOI":"10.1039\/D3LC00943B","article-title":"Sample\npreparation and detection\nmethods in point-of-care devices towards future at-home testing","volume":"24","author":"Adedokun","year":"2024","journal-title":"Lab Chip"},{"issue":"39","key":"2026081809072635300_cit21","doi-asserted-by":"publisher","first-page":"24450","DOI":"10.1073\/pnas.2011221117","article-title":"SARS-CoV-2 detection\nusing isothermal amplification and a rapid,\ninexpensive protocol for sample inactivation and purification","volume":"117","author":"Rabe","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"3","key":"2026081809072635300_cit22","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1016\/j.jbbm.2006.08.008","article-title":"Tolerance of loop-mediated\nisothermal amplification to a culture medium and biological substances","volume":"70","author":"Kaneko","year":"2007","journal-title":"J. Biochem Biophys Methods"},{"issue":"12","key":"2026081809072635300_cit23","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated\nisothermal amplification\nof DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"2026081809072635300_cit24","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.1021\/acs.analchem.8b04324","article-title":"Real-Time, Digital LAMP with Commercial\nMicrofluidic Chips Reveals the Interplay of Efficiency, Speed, and\nBackground Amplification as a Function of Reaction Temperature and\nTime","volume":"91","author":"Rolando","year":"2019","journal-title":"Anal. Chem."},{"issue":"7","key":"2026081809072635300_cit25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using\nrecombination proteins","volume":"4","author":"Piepenburg","year":"2006","journal-title":"PLoS Biol."},{"issue":"6313","key":"2026081809072635300_cit26","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1038\/350091a0","article-title":"Nucleic acid\nsequence-based amplification","volume":"350","author":"Compton","year":"1991","journal-title":"Nature"},{"issue":"8","key":"2026081809072635300_cit27","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1038\/sj.embor.7400200","article-title":"Helicase-dependent\nisothermal DNA amplification","volume":"5","author":"Vincent","year":"2004","journal-title":"EMBO Rep"},{"issue":"9","key":"2026081809072635300_cit28","doi-asserted-by":"publisher","first-page":"3132","DOI":"10.1128\/JCM.02046-10","article-title":"Multiplex real-time\nPCR melting curve assay to detect drug-resistant\nmutations of Mycobacterium tuberculosis","volume":"49","author":"Luo","year":"2011","journal-title":"J.\nClin Microbiol"},{"issue":"3","key":"2026081809072635300_cit29","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1006\/mcpr.2002.0415","article-title":"Accelerated reaction by loop-mediated\nisothermal amplification using loop primers","volume":"16","author":"Nagamine","year":"2002","journal-title":"Mol. Cell Probes"},{"issue":"12","key":"2026081809072635300_cit30","doi-asserted-by":"publisher","first-page":"6374","DOI":"10.3390\/ijms25126374","article-title":"Multiplexing\nLAMP Assays: A Methodological Review and Diagnostic Application","volume":"25","author":"Crego-Vicente","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"1","key":"2026081809072635300_cit31","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1186\/s12879-017-2382-0","article-title":"Point of\nsampling detection of Zika virus within a multiplexed kit capable\nof detecting dengue and chikungunya","volume":"17","author":"Yaren","year":"2017","journal-title":"BMC Infect.\nDis."},{"key":"2026081809072635300_cit32","doi-asserted-by":"publisher","first-page":"17925","DOI":"10.1038\/srep17925","article-title":"A novel multiplex\nisothermal amplification method for\nrapid detection and identification of viruses","volume":"5","author":"Nyan","year":"2015","journal-title":"Sci. Rep."},{"issue":"5","key":"2026081809072635300_cit33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006381","article-title":"Development\nand validation\nof four one-step real-time RT-LAMP assays for specific detection of\neach dengue virus serotype","volume":"12","author":"Lopez-Jimena","year":"2018","journal-title":"PLoS Negl Trop Dis"},{"key":"2026081809072635300_cit34","doi-asserted-by":"publisher","first-page":"44778","DOI":"10.1038\/srep44778","article-title":"A smartphone-based diagnostic platform\nfor rapid detection\nof Zika, chikungunya, and dengue viruses","volume":"7","author":"Priye","year":"2017","journal-title":"Sci.\nRep."},{"issue":"14","key":"2026081809072635300_cit35","doi-asserted-by":"publisher","first-page":"7289","DOI":"10.1021\/acs.analchem.6b01632","article-title":"Instrument-Free Point-of-Care\nMolecular Detection of Zika Virus","volume":"88","author":"Song","year":"2016","journal-title":"Anal. Chem."},{"issue":"12","key":"2026081809072635300_cit36","doi-asserted-by":"publisher","first-page":"714","DOI":"10.3390\/v10120714","article-title":"Simultaneous\nDetection of Different Zika Virus Lineages via Molecular Computation\nin a Point-of-Care Assay","volume":"10","author":"Bhadra","year":"2018","journal-title":"Viruses"},{"key":"2026081809072635300_cit37","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1186\/1471-2334-13-387","article-title":"Detection of dengue\nviruses using reverse transcription-loop-mediated isothermal amplification","volume":"13","author":"Teoh","year":"2013","journal-title":"BMC Infect. Dis."},{"key":"2026081809072635300_cit38","doi-asserted-by":"publisher","first-page":"44924","DOI":"10.1038\/srep44924","article-title":"Multiplex Reverse-Transcription Loop-Mediated Isothermal\nAmplification Coupled with Cascade Invasive Reaction and Nanoparticle\nHybridization for Subtyping of Influenza A Virus","volume":"7","author":"Chi","year":"2017","journal-title":"Sci. Rep."},{"issue":"8","key":"2026081809072635300_cit39","doi-asserted-by":"publisher","first-page":"1432","DOI":"10.3390\/diagnostics13081432","article-title":"Comparative\nClinical Evaluation of a Novel FluA\/FluB\/SARS-CoV-2 Multiplex LAMP\nand Commercial FluA\/FluB\/SARS-CoV-2\/RSV RT-qPCR Assays","volume":"13","author":"Jee","year":"2023","journal-title":"Diagnostics (Basel)"},{"key":"2026081809072635300_cit40","doi-asserted-by":"publisher","first-page":"114169","DOI":"10.1016\/j.bios.2022.114169","article-title":"Argonaute-integrated isothermal amplification\nfor rapid, portable, multiplex detection of SARS-CoV-2 and influenza\nviruses","volume":"207","author":"Ye","year":"2022","journal-title":"Biosens Bioelectron"},{"issue":"11","key":"2026081809072635300_cit41","doi-asserted-by":"publisher","first-page":"4176","DOI":"10.1021\/acssensors.1c01718","article-title":"A Valve-Enabled Sample Preparation\nDevice with Isothermal\nAmplification for Multiplexed Virus Detection at the Point-of-Care","volume":"6","author":"Manzanas","year":"2021","journal-title":"ACS Sens"},{"issue":"9","key":"2026081809072635300_cit42","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0238615","article-title":"Development\nof a multiplex isothermal\namplification molecular diagnosis method for on-site diagnosis of\ninfluenza","volume":"15","author":"Jang","year":"2020","journal-title":"PLoS One"},{"issue":"9","key":"2026081809072635300_cit43","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0011651","article-title":"The increasing\ncomplexity of arbovirus serology: An\nin-depth systematic review on cross-reactivity","volume":"17","author":"Kasbergen","year":"2023","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"2026081809072635300_cit44","doi-asserted-by":"publisher","first-page":"336","DOI":"10.3201\/eid3102.240791","article-title":"Dengue and Other\nArbovirus Infections among Schoolchildren, Haiti, 2021","volume":"31","author":"Louis","year":"2025","journal-title":"Emerg. Infect. Dis."},{"issue":"4","key":"2026081809072635300_cit45","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004687","article-title":"Zika Virus Outbreak in Haiti in 2014: Molecular and\nClinical Data","volume":"10","author":"Lednicky","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"6","key":"2026081809072635300_cit46","doi-asserted-by":"publisher","first-page":"919","DOI":"10.1093\/cid\/ciy582","article-title":"Clinical and Epidemiologic Patterns\nof Chikungunya Virus Infection and Coincident Arboviral Disease in\na School Cohort in Haiti, 2014\u20132015","volume":"68","author":"Ball","year":"2019","journal-title":"Clin Infect Dis"},{"key":"2026081809072635300_cit47","unstructured":"Pan American Health Organization. Epidemiological Update for Dengue,\nChikungunya and Zika in 2022. Available\nvia the Internet at: https:\/\/ais.paho.org\/ha_viz\/Arbo\/Arbo_Bulletin_2022.asp?env=pri (accessed Apr. 25, 2026)."},{"issue":"11","key":"2026081809072635300_cit48","doi-asserted-by":"publisher","first-page":"4873","DOI":"10.1021\/ac300476k","article-title":"Specific multiplex\nanalysis of pathogens using a direct 16S rRNA hybridization in microarray\nsystem","volume":"84","author":"Hwang","year":"2012","journal-title":"Analytical chemistry"},{"issue":"10","key":"2026081809072635300_cit49","doi-asserted-by":"publisher","first-page":"5428","DOI":"10.1021\/acs.analchem.7b00255","article-title":"Multiplex Paper-Based Colorimetric DNA Sensor Using\nPyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting\nMERS-CoV, MTB, and HPV Oligonucleotides","volume":"89","author":"Teengam","year":"2017","journal-title":"Analytical\nchemistry"},{"key":"2026081809072635300_cit50","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.bios.2014.05.039","article-title":"Brief review of\nmonitoring methods\nfor loop-mediated isothermal amplification (LAMP)","volume":"61","author":"Zhang","year":"2014","journal-title":"Biosens Bioelectron"},{"key":"2026081809072635300_cit51","doi-asserted-by":"publisher","first-page":"106009","DOI":"10.1016\/j.mimet.2020.106009","article-title":"A loop-mediated\nisothermal amplification\n(LAMP) assay for the consensus detection of human pathogenic Campylobacter\nspecies","volume":"176","author":"Babu","year":"2020","journal-title":"J. Microbiol Methods"},{"issue":"4","key":"2026081809072635300_cit52","doi-asserted-by":"publisher","first-page":"1376","DOI":"10.1128\/JCM.44.4.1376-1381.2006","article-title":"Rapid detection\nof norovirus from fecal specimens by real-time reverse transcription-loop-mediated\nisothermal amplification assay","volume":"44","author":"Fukuda","year":"2006","journal-title":"Journal of clinical\nmicrobiology"},{"issue":"1","key":"2026081809072635300_cit53","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.diagmicrobio.2015.05.004","article-title":"Development of a pan-serotype reverse\ntranscription loop-mediated isothermal amplification assay for the\ndetection of dengue virus","volume":"83","author":"Dauner","year":"2015","journal-title":"Diagn Microbiol Infect\nDis"},{"issue":"52","key":"2026081809072635300_cit54","doi-asserted-by":"publisher","first-page":"17211","DOI":"10.1002\/anie.201809993","article-title":"Valve-Enabled\nSample Preparation and RNA Amplification in a Coffee Mug for Zika\nVirus Detection","volume":"57","author":"Jiang","year":"2018","journal-title":"Angew. Chem., Int. Ed. Engl."},{"issue":"23","key":"2026081809072635300_cit55","doi-asserted-by":"publisher","first-page":"5605","DOI":"10.1007\/s00216-023-04856-8","article-title":"Mayaro virus detection by integrating\nsample preparation with isothermal amplification in portable devices","volume":"415","author":"Alipanah","year":"2023","journal-title":"Anal. Bioanal. Chem."},{"issue":"2","key":"2026081809072635300_cit56","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1128\/JCM.01734-06","article-title":"Rapid and real-time\ndetection of Chikungunya virus by reverse transcription loop-mediated\nisothermal amplification assay","volume":"45","author":"Parida","year":"2007","journal-title":"J. Clin Microbiol"},{"issue":"1","key":"2026081809072635300_cit57","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1038\/s41378-024-00822-1","article-title":"A handheld\nHIV detection platform using paper-based sample preparation and real-time\nisothermal amplification","volume":"10","author":"Adedokun","year":"2024","journal-title":"Microsyst. Nanoeng."},{"key":"2026081809072635300_cit58","unstructured":"Colosimo, F.; Tanner, N. A.; Patton, G. C.\n          LAMP Primer\nDesign using the NEB LAMP Primer Design Tool: Critical Considerations\nfor Assay Robustness, Speed and Sensitivity. Technical\nNote, New England Biolabs, 2025; pp 1.7."},{"issue":"1","key":"2026081809072635300_cit59","doi-asserted-by":"publisher","first-page":"21557","DOI":"10.1038\/s41598-023-48493-8","article-title":"Detection and persistence of Zika virus in body fluids and associated\nfactors: a prospective cohort study","volume":"13","author":"Calvet","year":"2023","journal-title":"Sci. Rep."},{"issue":"3","key":"2026081809072635300_cit60","doi-asserted-by":"publisher","first-page":"890","DOI":"10.1021\/acssynbio.4c00819","article-title":"Nanobody-Based\nLateral Flow Assay\nfor Rapid Zika Virus Detection","volume":"14","author":"Peng","year":"2025","journal-title":"ACS Synth. Biol."},{"issue":"5","key":"2026081809072635300_cit61","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0233309","article-title":"Short Report: Asymptomatic Zika virus infections with\nlow viral loads not likely to establish transmission in New Orleans\nAedes populations","volume":"15","author":"Ward","year":"2020","journal-title":"PLoS One"},{"issue":"40","key":"2026081809072635300_cit62","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01160-17","article-title":"Complete Genomic Sequence of\nDengue Virus Serotype 4 Isolated from\nPlasma Collected from a Haitian Child in 2014","volume":"5","author":"Elbadry","year":"2017","journal-title":"Genome Announc."},{"issue":"11","key":"2026081809072635300_cit63","doi-asserted-by":"publisher","first-page":"2000","DOI":"10.3201\/eid2211.161015","article-title":"Mayaro Virus\nin Child with Acute Febrile Illness, Haiti, 2015","volume":"22","author":"Lednicky","year":"2016","journal-title":"Emerg Infect Dis"},{"issue":"22","key":"2026081809072635300_cit64","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00331-17","article-title":"Complete Genomic\nSequence of Dengue virus 1, Isolated from Plasma Collected from a\nHaitian Child in 2014","volume":"5","author":"Elbadry","year":"2017","journal-title":"Genome Announc."},{"issue":"6","key":"2026081809072635300_cit65","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0234682","article-title":"Rapid detection\nof novel coronavirus\/Severe Acute Respiratory Syndrome Coronavirus\n2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification","volume":"15","author":"Lamb","year":"2020","journal-title":"PLoS One"},{"issue":"1","key":"2026081809072635300_cit66","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.jcv.2009.02.010","article-title":"Development\nand validation of real-time one-step reverse transcription-PCR for\nthe detection and typing of dengue viruses","volume":"45","author":"Leparc-Goffart","year":"2009","journal-title":"J. Clin Virol"},{"issue":"7","key":"2026081809072635300_cit67","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00405-24","article-title":"CDC Trioplex diagnostic\nassay underperforms in detection\nof circulating Chikungunya West African genotype","volume":"62","author":"Ndiaye","year":"2024","journal-title":"J. Clin Microbiol."},{"issue":"1","key":"2026081809072635300_cit68","doi-asserted-by":"publisher","first-page":"10321","DOI":"10.1038\/s41598-021-89070-1","article-title":"Genomic investigation of a dengue virus outbreak in\nThies, Senegal, in 2018","volume":"11","author":"Gaye","year":"2021","journal-title":"Sci. Rep."},{"issue":"8","key":"2026081809072635300_cit69","doi-asserted-by":"publisher","first-page":"1062","DOI":"10.1111\/j.1469-0691.2010.03279.x","article-title":"Point-of-care tests\nfor diagnosing infections in the developing world","volume":"16","author":"Peeling","year":"2010","journal-title":"Clin Microbiol Infect"},{"issue":"12","key":"2026081809072635300_cit70","doi-asserted-by":"publisher","first-page":"1249","DOI":"10.1016\/j.jiac.2020.07.001","article-title":"Simultaneous\ndiagnosis of dengue\nvirus, Chikungunya virus, and Zika virus infection using a new point-of-care\ntesting (POCT) system based on the loop-mediated isothermal amplification\n(LAMP) method","volume":"26","author":"Kutsuna","year":"2020","journal-title":"J. Infect Chemother"},{"issue":"3","key":"2026081809072635300_cit71","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1007\/s10156-013-0590-0","article-title":"Loop-mediated isothermal amplification\n(LAMP): recent progress in research and development","volume":"19","author":"Mori","year":"2013","journal-title":"J. Infect Chemother"},{"issue":"8","key":"2026081809072635300_cit72","doi-asserted-by":"publisher","first-page":"1232","DOI":"10.3201\/eid1408.080287","article-title":"Genetic and serologic\nproperties of Zika virus associated with an epidemic, Yap State, Micronesia,\n2007","volume":"14","author":"Lanciotti","year":"2008","journal-title":"Emerg Infect Dis"},{"issue":"6","key":"2026081809072635300_cit73","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001572","article-title":"Loop-mediated\nisothermal amplification technology: towards point of care diagnostics","volume":"6","author":"Njiru","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"2026081809072635300_cit74","doi-asserted-by":"publisher","first-page":"S30","DOI":"10.1038\/nrmicro2459","article-title":"Evaluation\nof diagnostic tests: dengue","volume":"8","author":"Peeling","year":"2010","journal-title":"Nat. Rev. Microbiol"},{"issue":"3","key":"2026081809072635300_cit75","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1038\/nrmicro841","article-title":"Diagnostics for\nthe developing world","volume":"2","author":"Mabey","year":"2004","journal-title":"Nat. Rev. Microbiol"},{"issue":"5","key":"2026081809072635300_cit76","doi-asserted-by":"publisher","first-page":"1144","DOI":"10.3390\/v15051144","article-title":"Genetic Analysis\nof Dengue Virus in Severe and Non-Severe Cases in Dhaka, Bangladesh,\nin 2018\u20132022","volume":"15","author":"Rahim","year":"2023","journal-title":"Viruses"},{"key":"2026081809072635300_cit77","doi-asserted-by":"publisher","first-page":"107299","DOI":"10.1016\/j.ijid.2024.107299","article-title":"Evaluating a rapid molecular assay in a mobile laboratory\nfor improved diagnosis of dengue in Bangladesh","volume":"150","author":"Hossain","year":"2025","journal-title":"Int. J. Infect Dis"},{"issue":"7","key":"2026081809072635300_cit78","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1089\/vbz.2015.1899","article-title":"Genetic\nVariability in Probe Binding Regions Explains False Negative Results\nof a Molecular Assay for the Detection of Dengue Virus","volume":"16","author":"Koo","year":"2016","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"1","key":"2026081809072635300_cit79","doi-asserted-by":"publisher","first-page":"18108","DOI":"10.1038\/s41598-021-97534-7","article-title":"Nested pool\ntesting strategy for the diagnosis of infectious\ndiseases","volume":"11","author":"Armendariz","year":"2021","journal-title":"Sci. Rep."},{"issue":"589","key":"2026081809072635300_cit80","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abf1568","article-title":"Using viral load\nand epidemic dynamics to optimize pooled testing in resource-constrained\nsettings","volume":"13","author":"Cleary","year":"2021","journal-title":"Sci. Transl Med."},{"issue":"1","key":"2026081809072635300_cit81","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1021\/ac9013989","article-title":"Diagnostics for the\ndeveloping world: microfluidic paper-based analytical devices","volume":"82","author":"Martinez","year":"2010","journal-title":"Analytical chemistry"},{"issue":"1","key":"2026081809072635300_cit82","doi-asserted-by":"publisher","first-page":"8479","DOI":"10.1038\/s41467-023-44199-7","article-title":"Quantitatively assessing\nearly detection strategies for mitigating COVID-19 and future pandemics","volume":"14","author":"Liu","year":"2023","journal-title":"Nat. Commun."},{"issue":"4","key":"2026081809072635300_cit83","doi-asserted-by":"publisher","first-page":"860","DOI":"10.1128\/JCM.00279-16","article-title":"Zika Virus: Diagnostics for an Emerging\nPandemic Threat","volume":"54","author":"Waggoner","year":"2016","journal-title":"J. Clin Microbiol"},{"key":"2026081809072635300_cit84","unstructured":"US Centers for Disease Control and Prevention,\nDengue Clinical Management\nPocket Guide. Available via the Internet at: https:\/\/www.cdc.gov\/dengue\/hcp\/pocketguide\/ (accessed May 14, 2026)."},{"issue":"3","key":"2026081809072635300_cit85","doi-asserted-by":"publisher","first-page":"542","DOI":"10.4269\/ajtmh.23-0205","article-title":"Therapy for Chikungunya Arthritis: A Study of 133 Brazilian\nPatients","volume":"109","author":"Amaral","year":"2023","journal-title":"Am. J. Trop Med. Hyg"},{"issue":"2","key":"2026081809072635300_cit86","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1007\/s12098-022-04394-8","article-title":"Management of Dengue: An Updated\nReview","volume":"90","author":"Tayal","year":"2023","journal-title":"Indian J. Pediatr"},{"issue":"5","key":"2026081809072635300_cit87","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1080\/14728222.2024.2351504","article-title":"Current challenges\nin the discovery of treatments against Mayaro fever","volume":"28","author":"Marques","year":"2024","journal-title":"Expert Opin Ther Targets"},{"issue":"3","key":"2026081809072635300_cit88","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003603","article-title":"Specific management\nof post-chikungunya rheumatic disorders: a retrospective\nstudy of 159 cases in Reunion Island from 2006\u20132012","volume":"9","author":"Javelle","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"issue":"10","key":"2026081809072635300_cit89","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1056\/NEJMoa1600651","article-title":"Zika Virus\nAssociated with Microcephaly","volume":"374","author":"Mlakar","year":"2016","journal-title":"N Engl J. Med."},{"issue":"1","key":"2026081809072635300_cit90","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000130","article-title":"Arbovirus coinfection and co-transmission: A neglected\npublic health concern?","volume":"17","author":"Vogels","year":"2019","journal-title":"PLoS Biol."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.5c08263","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/32\/23450\/66379400\/acs.analchem.5c08263.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/32\/23450\/66379400\/acs.analchem.5c08263.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:09:14Z","timestamp":1787069354000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/32\/23450\/5242281\/Multiplexed-RT-LAMP-Assays-in-Handheld-Devices-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":90,"journal-issue":{"issue":"32","published-online":{"date-parts":[[2026,8,4]]},"published-print":{"date-parts":[[2026,8,18]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.5c08263","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,4]]}}}