{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T16:04:09Z","timestamp":1789488249015,"version":"build-2803163510"},"reference-count":34,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The size characterization of nano-objects in the 0.1\u2013300 nm range can be achieved by Taylor dispersion analysis (TDA), with typical analysis times of 10\u201320 min in capillaries with an internal diameter of 50 \u03bcm. In TDA, longer detection times lead to improved detection sensitivity. To increase throughput while preserving sensitivity, sequential TDA is investigated, in which successive injections are performed within a single experiment without waiting for complete elution of the previous sample. In this work, the feasibility of sequential TDA applied to different protein samples is evaluated, using thyroglobulin and \u03b3-globulin as model compounds. This strategy provides significantly higher analytical throughput without compromising detection sensitivity or the accessible sizing range of TDA. Based on five to six successive injections performed in a single run, the total analysis time can be reduced by a factor of more than three. Sequential analysis is not restricted to Gaussian fitting and can be also analyzed using Constrained Regularized Linear Inversion (CRLI).<\/jats:p>","DOI":"10.1021\/acs.analchem.6c00765","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T16:16:48Z","timestamp":1788452208000},"page":"26353-26359","source":"Crossref","is-referenced-by-count":0,"title":["High-throughput and sensitive Taylor dispersion analysis\nof proteins using sequential injections"],"prefix":"10.1021","volume":"98","author":[{"given":"Jean-Philippe","family":"Biron","sequence":"first","affiliation":[{"name":"University of Montpellier, CNRS, ENSCM , , ,","place":["Montpellier, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6876-175X","authenticated-orcid":true,"given":"Herve\u0301","family":"Cottet","sequence":"additional","affiliation":[{"name":"University of Montpellier, CNRS, ENSCM , , ,","place":["Montpellier, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"2026091511071683600_cit1","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1098\/rspa.1953.0139","article-title":"Dispersion\nof soluble matter in solvent flowing slowly through a tube","volume":"219","author":"Taylor","year":"1953","journal-title":"Proceedings of the Royal Society of London, A"},{"key":"2026091511071683600_cit2","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1126\/science.266.5186.773","article-title":"Use of taylor-aris dispersion for\nmeasurement of a solute diffusion coefficient in thin capillaries","volume":"266","author":"Bello","year":"1994","journal-title":"Science."},{"key":"2026091511071683600_cit3","doi-asserted-by":"publisher","first-page":"3552","DOI":"10.1039\/C4AN00192C","article-title":"On the optimization\nof operating\nconditions for Taylor dispersion analysis of mixtures","volume":"139","author":"Cottet","year":"2014","journal-title":"Analyst."},{"key":"2026091511071683600_cit4","doi-asserted-by":"publisher","first-page":"13487","DOI":"10.1021\/acs.analchem.7b03806","article-title":"Limits in size of Taylor dispersion\nanalysis: representation of the different hydrodynamic regimes and\napplication to the size-characterization of cubosomes","volume":"89","author":"Chamieh","year":"2017","journal-title":"Anal. Chem."},{"key":"2026091511071683600_cit5","doi-asserted-by":"publisher","first-page":"2357","DOI":"10.1039\/D1AY00588J","article-title":"Taylor dispersion analysis in fused\nsilica capillaries:\na tutorial review","volume":"13","author":"Moser","year":"2021","journal-title":"Anal Methods."},{"key":"2026091511071683600_cit6","doi-asserted-by":"publisher","first-page":"1969","DOI":"10.1002\/app.1991.070420722","article-title":"Flow injection analysis\nestimation of diffusion coefficients of pauci-and polydisperse polymers\nsuch as polystyrene sulfonates","volume":"42","author":"Boyle","year":"1991","journal-title":"J. Appl. Polym.\nSci."},{"key":"2026091511071683600_cit7","doi-asserted-by":"publisher","first-page":"462949","DOI":"10.1016\/j.chroma.2022.462949","article-title":"Determination\nof ultrahigh\nmolar mass of polyelectrolytes by Taylor dispersion analysis","volume":"1670","author":"Leclercq","year":"2022","journal-title":"J. Chromatogr. 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Sci."},{"key":"2026091511071683600_cit31","doi-asserted-by":"publisher","first-page":"465325","DOI":"10.1016\/j.chroma.2024.465325","article-title":"Preventing\nthe impact of solute adsorption in Taylor dispersion analysis: Application\nto protein and lipid nanoparticle analysis","volume":"1736","author":"Roca","year":"2024","journal-title":"J. Chromatogr. A"},{"key":"2026091511071683600_cit32","doi-asserted-by":"publisher","first-page":"8489","DOI":"10.1021\/acs.analchem.5b02053","article-title":"Measuring Arbitrary\nDiffusion Coefficient Distributions of Nano-Objects by Taylor Dispersion\nAnalysis","volume":"87","author":"Cipelletti","year":"2015","journal-title":"Anal. Chem."},{"key":"2026091511071683600_cit33","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/j.polymertesting.2018.01.017","article-title":"Size-characterization\nof natural and synthetic polyisoprenes by Taylor dispersion analysis","volume":"66","author":"Biron","year":"2018","journal-title":"Polymer Testing."},{"key":"2026091511071683600_cit34","unstructured":"Lebedev, I.; Brill, L.; Rivera, B.; Tackett, B.\n          Size\nExclusion Chromatography\nfor the Determination of Protein Hydrodynamic Radius\u2500Revisiting\nthe Calibration Curve Size-characterization of natural and synthetic\npolyisoprenes by Taylor dispersion analysis. White\nPaper, Phenomenex, 2021."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c00765","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/36\/26353\/68189163\/acs.analchem.6c00765.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/36\/26353\/68189163\/acs.analchem.6c00765.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:20:22Z","timestamp":1789485622000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/36\/26353\/5404747\/High-throughput-and-sensitive-Taylor-dispersion"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,2]]},"references-count":34,"journal-issue":{"issue":"36","published-online":{"date-parts":[[2026,9,2]]},"published-print":{"date-parts":[[2026,9,15]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c00765","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,2]]}}}