{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T16:06:34Z","timestamp":1789488394748,"version":"build-2803163510"},"reference-count":81,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"name":"Military Infectious Diseases Research Program","award":["HT9425-23-C-0103"],"award-info":[{"award-number":["HT9425-23-C-0103"]}]},{"name":"Military Infectious Diseases Research Program","award":["MI230112"],"award-info":[{"award-number":["MI230112"]}]},{"name":"National Institutes of Health (NIH)","award":["U54EB034654"],"award-info":[{"award-number":["U54EB034654"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ebola virus (EBOV) and Lassa virus (LASV) infections present with indistinguishable early symptoms, creating a persistent diagnostic gap in coendemic regions. This challenge is compounded in resource-limited settings, where existing lateral flow assays are single-target, qualitative, and reliant on subjective interpretation. We developed a duplex colorimetric lateral flow immunoassay (LFIA) for simultaneous detection of EBOV VP40 and LASV nucleoprotein (NP), integrated with the Portable Rapid Onsite Versatile Image-based Detection System (PROVIDS), a compact optical reader that automates line segmentation, trichannel deconvolution, and test-to-control ratio quantification in under 5 s. The assay achieved limits of detection of 44 ng\/mL for EBOV and 69 ng\/mL for LASV, with intra-assay CVs below 10%, retained full analytical performance after 8 weeks of ambient storage, and showed high specificity for Zaire-lineage EBOV and LASV lineages II (Macenta) and IV (Josiah) without cross-reactivity to Marburg virus (MARV). Meeting WHO Target Product Profile (TPP) benchmarks, this PROVIDS-enabled duplex LFIA provides a portable, quantitative, and multiplexed platform for outbreak triage and establishes a scalable foundation for future syndromic surveillance of viral hemorrhagic fevers (VHFs).<\/jats:p>","DOI":"10.1021\/acs.analchem.6c00820","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:39:37Z","timestamp":1788406777000},"page":"26360-26372","source":"Crossref","is-referenced-by-count":0,"title":["Portable Duplex\nDiagnostic Platform for Point-of-Care\nDifferentiation of Ebola and Lassa Virus"],"prefix":"10.1021","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2356-5244","authenticated-orcid":true,"given":"Wenting","family":"Gao","sequence":"first","affiliation":[{"name":"Cornell University , , 121 Weill Hall, 237 Tower Road , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theopisti A.","family":"Nikolaou","sequence":"additional","affiliation":[{"name":"Cornell University , , 121 Weill Hall, 237 Tower Road , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monalisa","family":"Almeida","sequence":"additional","affiliation":[{"name":"Cornell University , , 121 Weill Hall, 237 Tower Road , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber","family":"Consul","sequence":"additional","affiliation":[{"name":"U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID) , 1425 Porter Street, Fort Detrick , , ,","place":["Frederick, Maryland, United States, 21702-5011"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunting","family":"Guo","sequence":"additional","affiliation":[{"name":"Cornell University , , Upson Hall, 124 Hoy Road , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Olguin","sequence":"additional","affiliation":[{"name":"U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID) , 1425 Porter Street, Fort Detrick , , ,","place":["Frederick, Maryland, United States, 21702-5011"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarence","family":"Un","sequence":"additional","affiliation":[{"name":"Cornell University , , Klarman Hall, 232 Feeney Way , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noon","family":"Elbedawi","sequence":"additional","affiliation":[{"name":"Cornell University , , 121 Weill Hall, 237 Tower Road , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antra","family":"Ganguly","sequence":"additional","affiliation":[{"name":"Cornell University , 3219 Martha Van Rensselaer Hall, 116 Reservoir Avenue , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maguette","family":"Diop","sequence":"additional","affiliation":[{"name":"Cornell University , , Upson Hall, 124 Hoy Road , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3788-0665","authenticated-orcid":true,"given":"Saurabh","family":"Mehta","sequence":"additional","affiliation":[{"name":"Cornell University , 3219 Martha Van Rensselaer Hall, 116 Reservoir Avenue , , ,","place":["Ithaca, New York, United States, 14853"]},{"name":"Cornell University , , 369 Upson Hall, 124 Hoy Road , , ,","place":["Ithaca, New York, United States, 14850"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keersten","family":"Ricks","sequence":"additional","affiliation":[{"name":"U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID) , 1425 Porter Street, Fort Detrick , , ,","place":["Frederick, Maryland, United States, 21702-5011"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1624-9711","authenticated-orcid":true,"given":"David","family":"Erickson","sequence":"additional","affiliation":[{"name":"Cornell University , , Upson Hall, 124 Hoy Road , , ,","place":["Ithaca, New York, United States, 14853"]},{"name":"Cornell University , 3219 Martha Van Rensselaer Hall, 116 Reservoir Avenue , , ,","place":["Ithaca, New York, United States, 14853"]},{"name":"Cornell University , , 369 Upson Hall, 124 Hoy Road , , ,","place":["Ithaca, New York, United States, 14850"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"2026091511074984800_cit1","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1016\/S0140-6736(10)60667-8","article-title":"Ebola haemorrhagic\nfever","volume":"377","author":"Feldmann","year":"2011","journal-title":"The\nLancet"},{"key":"2026091511074984800_cit2","doi-asserted-by":"publisher","first-page":"2079","DOI":"10.3201\/eid1512.091044","article-title":"Filoviruses:\nA Compendium of 40 Years of Epidemiological, Clinical, and Laboratory\nStudies","volume":"15","author":"Rollin","year":"2009","journal-title":"Emerging Infect. 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