{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T14:24:40Z","timestamp":1794493480403,"version":"4.9.1"},"reference-count":46,"publisher":"American Chemical Society (ACS)","issue":"22","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004073","name":"Killam Trusts","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004073","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020797","name":"NanoMedicines Innovation Network","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020797","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007711","name":"British Columbia Knowledge Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004489","name":"Mitacs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004489","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Sciences and Engineering Research Council of Canada (NSERC)"},{"name":"Canadian Institutes of Health Research (CIHR)","award":["FBD 193487"],"award-info":[{"award-number":["FBD 193487"]}]},{"name":"Swedish Research Council (VR)","award":["2024-00439"],"award-info":[{"award-number":["2024-00439"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anal. Chem."],"published-print":{"date-parts":[[2026,6,9]]},"DOI":"10.1021\/acs.analchem.6c00937","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T04:33:58Z","timestamp":1780029238000},"page":"15961-15967","source":"Crossref","is-referenced-by-count":3,"title":["Direct Counting of mRNA Copies Inside Individual Lipid Nanoparticles Using In Situ Lysis and Labeling"],"prefix":"10.1021","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3157-9446","authenticated-orcid":true,"given":"Sadie","family":"Graves","sequence":"first","affiliation":[{"name":"Michael Smith Laboratories","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]},{"name":"Department of Physics and Astronomy","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]},{"name":"School of Biomedical Engineering","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6020-9786","authenticated-orcid":true,"given":"Martin","family":"Jasinski","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]},{"name":"Department of Physics and Astronomy","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4002-0917","authenticated-orcid":true,"given":"Erik","family":"Ols\u00e9n","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]},{"name":"Department of Physics","place":["Gothenburg, Sweden"]},{"name":"University of Gothenburg","place":["Gothenburg, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6790-1140","authenticated-orcid":true,"given":"Albert","family":"Kamanzi","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]},{"name":"ScopeSys, Inc.","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2783-1317","authenticated-orcid":true,"given":"Yao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]},{"name":"School of Biomedical Engineering","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]},{"name":"Department of Biochemistry and Molecular Biology","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry","family":"Leung","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]},{"name":"Department of Biochemistry and Molecular Biology","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Venier-Karzis","sequence":"additional","affiliation":[{"name":"ScopeSys, Inc.","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6500-5086","authenticated-orcid":true,"given":"Amin","family":"Safaeesirat","sequence":"additional","affiliation":[{"name":"Department of Physics","place":["Burnaby, Canada"]},{"name":"Simon Fraser University","place":["Burnaby, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9586-2508","authenticated-orcid":true,"given":"Pieter R.","family":"Cullis","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4336-0447","authenticated-orcid":true,"given":"Sabrina R.","family":"Leslie","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]},{"name":"Department of Physics and Astronomy","place":["Vancouver, Canada"]},{"name":"University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,5,29]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-024-00977-6"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1039\/D4NR00019F"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1002\/bmm2.12116"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c06296"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1007\/s40005-022-00569-9"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.3390\/v17040566"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.2c00032"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2024.07.046"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2024.06.158"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2024.124097"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1002\/admi.202301083"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-023-01663-y"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01825-5"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.4c20077"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c02341"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.5c17445"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2018.03.010"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1021\/jp049684d"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.6b00053"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202110137"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz084"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1021\/nn505162u"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2023.11.017"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2025.114082"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2021.03.012"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c04862"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1021\/ac101041s"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-52685-1"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.8b01516"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.5b02891"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c12981"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b13349"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202303370"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33157-4"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202206187"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-01728-8"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.5c02232"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02441-2"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1720542115"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202105832"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2426601122"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1111\/bph.70340"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2016.10.014"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2021.120586"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines12030318"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-022-00549-y"}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c00937","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c00937","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T08:26:31Z","timestamp":1780993591000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.6c00937"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,29]]},"references-count":46,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2026,6,9]]}},"alternative-id":["10.1021\/acs.analchem.6c00937"],"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c00937","relation":{"has-part":[{"id-type":"doi","id":"https:\/\/doi.org\/10.1021\/acs.analchem.6c00937.s001","asserted-by":"subject"}]},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,29]]}}}