{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:43:24Z","timestamp":1792784604370,"version":"4.5.0"},"reference-count":44,"publisher":"American Chemical Society (ACS)","issue":"38","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009318","name":"Helmholtz Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009318","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Regional Development Fund (ERDF \/ EFRE)"},{"name":"Bundesministerium f?r Bildung und Forschung","id":[{"id":"https:\/\/ror.org\/04pz7b180","id-type":"ROR","asserted-by":"crossref"}]},{"name":"China Scholarship Council (CSC)","id":[{"id":"https:\/\/ror.org\/04atp4p48","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Formalin-fixed, paraffin-embedded (FFPE) tissues constitute the primary material for diagnostic pathology and retrospective clinical research, yet their use in metabolomics remains limited due to molecular cross-linking and analyte degradation. Here, we establish a cost-efficient molecular pathology workflow that integrates ultra-high-performance liquid chromatography mass spectrometry (UHPLC-MS) with matrix-assisted laser desorption\/ionization mass spectrometry imaging (MALDI-MSI) to quantify and spatially map nucleosides in FFPE breast cancer tissues. Optimized extraction using methanol yielded nucleoside profiles comparable to fresh-frozen tissues, while MALDI-MSI enabled the spatial visualization of nine nucleosides across distinct histological regions. Several nucleosides including deoxyadenosine and 5-formylcytosine showed strong discriminatory power between tumor stages, revealing progressive metabolic rewiring during breast cancer progression. Finally, spatial nucleoside patterns observed in a murine model were recapitulated in patient-derived FFPE tissues, underscoring the translational potential of nucleoside-based spatial metabolomics for clinical research and biomarker discovery. Together, this workflow establishes MALDI-MSI as a powerful and scalable spatial molecular pathology tool for interrogating nucleoside biology in archival breast cancer samples. Following MALDI-MSI, the same FFPE tissue sections can undergo laser capture microdissection, enabling genomic, proteomic, or targeted metabolomic profiling of MSI-defined tumor niches and microenvironmental regions. This integration directly links spatial nucleoside signatures to molecular alterations relevant to precision oncology in future.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c00978","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T20:02:35Z","timestamp":1789675355000},"page":"27906-27917","source":"Crossref","is-referenced-by-count":0,"title":["Integrating\nUHPLC-MS and MALDI-MSI for Spatial Nucleoside\nProfiling in FFPE Breast Cancer: A Multimodal Molecular Pathology\nFramework"],"prefix":"10.1021","volume":"98","author":[{"given":"Jierong","family":"Wu","sequence":"first","affiliation":[{"name":"Max Delbru\u0308ck Center for Molecular Medicine in the Helmholtz Association (MDC) , , ,","place":["Berlin, Germany, 13125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Geisberger","sequence":"additional","affiliation":[{"name":"Max Delbru\u0308ck Center for Molecular Medicine in the Helmholtz Association (MDC) , , ,","place":["Berlin, Germany, 13125"]},{"name":"MDC-Bruker Center of Excellence for Single Cell OMICS , ,","place":["Berlin, Germany, 10115"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Mastrobuoni","sequence":"additional","affiliation":[{"name":"Max Delbru\u0308ck Center for Molecular Medicine in the Helmholtz Association (MDC) , , ,","place":["Berlin, Germany, 13125"]},{"name":"MDC-Bruker Center of Excellence for Single Cell OMICS , ,","place":["Berlin, Germany, 10115"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamil","family":"Lisek","sequence":"additional","affiliation":[{"name":"Max Delbru\u0308ck Center for Molecular Medicine in the Helmholtz Association (MDC) , , ,","place":["Berlin, Germany, 13125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Raimundo","sequence":"additional","affiliation":[{"name":"Max Delbru\u0308ck Center for Molecular Medicine in the Helmholtz Association (MDC) , , ,","place":["Berlin, Germany, 13125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grit","family":"Nebrich","sequence":"additional","affiliation":[{"name":"Berlin Institute of Health at Charite\u0301\u2500Universita\u0308tsmedizin Berlin , , ,","place":["Berlin, Germany, 13353"]},{"name":"Berlin Institute of Health at Charite\u0301\u2500Universita\u0308tsmedizin Berlin , , ,","place":["Berlin, Germany, 13353"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Grzeski","sequence":"additional","affiliation":[{"name":"Berlin Institute of Health at Charite\u0301\u2500Universita\u0308tsmedizin Berlin , , ,","place":["Berlin, Germany, 13353"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaus","family":"Rajewsky","sequence":"additional","affiliation":[{"name":"Max Delbru\u0308ck Center for Molecular Medicine in the Helmholtz Association (MDC) , , ,","place":["Berlin, Germany, 13125"]},{"name":"Charite\u0301\u2500Universita\u0308tsmedizin Berlin, Corporate Member of Freie Universita\u0308t Berlin and Humboldt-Universita\u0308t zu Berlin , ,","place":["Berlin, Germany, 10117"]},{"name":"Charite\u0301\u2500Universita\u0308tsmedizin Berlin , , ,","place":["Berlin, Germany, 10117"]},{"name":"German Center for Cardiovascular Research (DZHK), Partner Site Berlin , ,","place":["Berlin, Germany, 10117"]},{"name":"German Cancer Consortium (DKTK) , ,","place":["Heidelberg, Germany, 69120"]},{"name":"National Center for Tumor Diseases (NCT), Partner Site Berlin , ,","place":["Berlin, Germany, 10117"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick","family":"Klauschen","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Ludwig Maximilians University Hospital Munich , ,","place":["Munich, Germany, 80336"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Klein","sequence":"additional","affiliation":[{"name":"Berlin Institute of Health at Charite\u0301\u2500Universita\u0308tsmedizin Berlin , , ,","place":["Berlin, Germany, 13353"]},{"name":"Berlin Institute of Health at Charite\u0301\u2500Universita\u0308tsmedizin Berlin , , ,","place":["Berlin, Germany, 13353"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0696-9299","authenticated-orcid":true,"given":"Stefan","family":"Kempa","sequence":"additional","affiliation":[{"name":"Max Delbru\u0308ck Center for Molecular Medicine in the Helmholtz Association (MDC) , , ,","place":["Berlin, Germany, 13125"]},{"name":"MDC-Bruker Center of Excellence for Single Cell OMICS , ,","place":["Berlin, Germany, 10115"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"2026092909092380400_cit1","doi-asserted-by":"publisher","first-page":"33","DOI":"10.20517\/cdr.2024.77","article-title":"Current applications\nof tumor local ablation (TLA) combined with immune checkpoint inhibitors\nin breast cancer treatment","volume":"7","author":"Tang","year":"2024","journal-title":"Cancer Drug Resist"},{"issue":"16","key":"2026092909092380400_cit2","doi-asserted-by":"publisher","first-page":"4156","DOI":"10.1021\/ol201073e","article-title":"Protection-free one-pot\nsynthesis of 2\u2019-deoxynucleoside 5\u2019-triphosphates and\nDNA polymerization","volume":"13","author":"Caton-Williams","year":"2011","journal-title":"Org. Lett."},{"issue":"8","key":"2026092909092380400_cit3","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1016\/j.trecan.2023.04.008","article-title":"Emerging roles of\nnucleotide metabolism in cancer","volume":"9","author":"Shi","year":"2023","journal-title":"Trends Cancer"},{"issue":"9","key":"2026092909092380400_cit4","doi-asserted-by":"publisher","first-page":"2155","DOI":"10.1111\/febs.17278","article-title":"Harnessing nucleotide\nmetabolism and immunity in cancer:\na tumour microenvironment perspective","volume":"292","author":"Sulieman","year":"2024","journal-title":"FEBS\nJ."},{"issue":"12","key":"2026092909092380400_cit5","doi-asserted-by":"publisher","first-page":"3263","DOI":"10.1093\/jxb\/eraf232","article-title":"A novel\nmolecular switch in plant growth: how adenosine kinase activity modulates\nplant development and growth pathways","volume":"76","author":"Watson","year":"2025","journal-title":"J. Exp\nBot"},{"key":"2026092909092380400_cit6","doi-asserted-by":"publisher","first-page":"1297826","DOI":"10.3389\/fmolb.2023.1297826","article-title":"Mechano-induced cell\nmetabolism disrupts the oxidative stress homeostasis\nof SAOS-2 osteosarcoma cells","volume":"10","author":"Fanelli","year":"2024","journal-title":"Front. Mol. Biosci"},{"issue":"17","key":"2026092909092380400_cit7","doi-asserted-by":"publisher","first-page":"9521","DOI":"10.3390\/ijms23179521","article-title":"Advances of Epigenetic Biomarkers\nand Epigenome Editing for Early\nDiagnosis in Breast Cancer","volume":"23","author":"Sarvari","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"11","key":"2026092909092380400_cit8","doi-asserted-by":"publisher","first-page":"1543","DOI":"10.1002\/rcm.4034","article-title":"Analysis of urinary\nnucleosides as\nhelper tumor markers in hepatocellular carcinoma diagnosis","volume":"23","author":"Jeng","year":"2009","journal-title":"Rapid Commun. Mass Spectrom"},{"issue":"12","key":"2026092909092380400_cit9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081701","article-title":"Urinary nucleosides as biomarkers of breast, colon,\nlung, and gastric cancer in Taiwanese","volume":"8","author":"Hsu","year":"2013","journal-title":"PLoS\nOne"},{"key":"2026092909092380400_cit10","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1186\/1471-2407-9-104","article-title":"Prediction\nof breast cancer by profiling\nof urinary RNA metabolites using Support Vector Machine-based feature\nselection","volume":"9","author":"Henneges","year":"2009","journal-title":"BMC Cancer"},{"issue":"6797","key":"2026092909092380400_cit11","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1038\/35021093","article-title":"Molecular\nportraits of human breast tumours","volume":"406","author":"Perou","year":"2000","journal-title":"Nature"},{"issue":"11","key":"2026092909092380400_cit12","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.3390\/ph15111307","article-title":"Evaluation\nof Formalin-Fixed and FFPE Tissues for Spatially Resolved Metabolomics\nand Drug Distribution Studies","volume":"15","author":"Dannhorn","year":"2022","journal-title":"Pharmaceuticals"},{"issue":"3","key":"2026092909092380400_cit13","doi-asserted-by":"publisher","DOI":"10.1038\/oncsis.2017.2","article-title":"Hyperglycemia exacerbates colon cancer\nmalignancy through hexosamine biosynthetic pathway","volume":"6","author":"Vasconcelos-Dos-Santos","year":"2017","journal-title":"Oncogenesis"},{"issue":"5","key":"2026092909092380400_cit14","doi-asserted-by":"publisher","DOI":"10.1172\/JCI145107","article-title":"Visualizing the dynamics of tuberculosis\npathology using molecular imaging","volume":"131","author":"Ordonez","year":"2021","journal-title":"J. Clin Invest"},{"issue":"1","key":"2026092909092380400_cit15","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1186\/s12935-023-03027-0","article-title":"Spatially resolved\nvisualization of reprogrammed metabolism in hepatocellular\ncarcinoma by mass spectrometry imaging","volume":"23","author":"Ma","year":"2023","journal-title":"Cancer.\nCell Int."},{"issue":"1","key":"2026092909092380400_cit16","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.molonc.2013.08.010","article-title":"Chemical\nmapping of the colorectal cancer microenvironment via MALDI imaging\nmass spectrometry (MALDI-MSI) reveals novel cancer-associated field\neffects","volume":"8","author":"Mirnezami","year":"2014","journal-title":"Mol. Oncol"},{"issue":"5","key":"2026092909092380400_cit17","doi-asserted-by":"publisher","first-page":"1145","DOI":"10.1158\/1535-7163.MCT-15-0165","article-title":"Modeling Therapy\nResponse and Spatial Tissue Distribution of Erlotinib in Pancreatic\nCancer","volume":"15","author":"Gru\u0308ner","year":"2016","journal-title":"Mol. Cancer Ther"},{"key":"2026092909092380400_cit18","doi-asserted-by":"publisher","first-page":"891018","DOI":"10.3389\/fonc.2022.891018","article-title":"Comparing\nDESI-MSI and MALDI-MSI Mediated Spatial Metabolomics and Their Applications\nin Cancer Studies","volume":"12","author":"He","year":"2022","journal-title":"Front Oncol"},{"issue":"8","key":"2026092909092380400_cit19","doi-asserted-by":"publisher","first-page":"1428","DOI":"10.1038\/nprot.2016.081","article-title":"High-Mass-Resolution MALDI Mass Spectrometry\nImaging of Metabolites from Formalin-Fixed Paraffin-Embedded Tissue","volume":"11","author":"Ly","year":"2016","journal-title":"Nat. Protoc"},{"key":"2026092909092380400_cit20","doi-asserted-by":"publisher","DOI":"10.1101\/2025.07.15.662972","article-title":"Spatiotemporal dynamics\nof tumor microenvironment remodeling","author":"Lisek","year":"2025","journal-title":"bioRxiv\nposted"},{"issue":"4","key":"2026092909092380400_cit21","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1158\/1541-7786.MCR-16-0262","article-title":"Metabolic\nProfiling in Formalin-Fixed\nand Paraffin-Embedded Prostate Cancer Tissues","volume":"15","author":"Cacciatore","year":"2017","journal-title":"Mol. Cancer Res."},{"issue":"15","key":"2026092909092380400_cit22","doi-asserted-by":"publisher","first-page":"4693","DOI":"10.1016\/j.jprot.2012.02.012","article-title":"MALDI Imaging Mass Spectrometry Reveals COX7A2, TAGLN2 and S100-A10\nas Novel Prognostic Markers in Barrett\u2019s Adenocarcinoma","volume":"75","author":"Elsner","year":"2012","journal-title":"J. Proteomics"},{"issue":"20","key":"2026092909092380400_cit23","doi-asserted-by":"publisher","first-page":"2249","DOI":"10.1002\/pmic.201400088","article-title":"MALDI Imaging Mass Spectrometry:\nDiscrimination of\nPathophysiological Regions in Traumatized Skeletal Muscle by Characteristic\nPeptide Signatures","volume":"14","author":"Klein","year":"2014","journal-title":"Proteomics"},{"key":"2026092909092380400_cit24","doi-asserted-by":"publisher","first-page":"102649","DOI":"10.1016\/j.dnarep.2019.102649","article-title":"Formation and Biological\nConsequences of 5-Formylcytosine in Genomic\nDNA","volume":"81","author":"Zhang","year":"2019","journal-title":"DNA Repair (Amst)"},{"key":"2026092909092380400_cit25","doi-asserted-by":"publisher","first-page":"1697","DOI":"10.3389\/fimmu.2018.01697","article-title":"Potential\nMechanisms Connecting Purine Metabolism and Cancer Therapy","volume":"9","author":"Yin","year":"2018","journal-title":"Front Immunol"},{"key":"2026092909092380400_cit26","doi-asserted-by":"publisher","first-page":"115080","DOI":"10.1016\/j.bcp.2022.115080","article-title":"Spatial Analysis\nof Drug Absorption, Distribution, Metabolism, and Toxicology Using\nMass Spectrometry Imaging","volume":"201","author":"Spruill","year":"2022","journal-title":"Biochem Pharmacol"},{"issue":"4","key":"2026092909092380400_cit27","doi-asserted-by":"publisher","first-page":"2285","DOI":"10.1021\/acs.analchem.2c03953","article-title":"Using the Synergy between HPLC-MS\nand MALDI-MS Imaging to Explore the Lipidomics of Clear Cell Renal\nCell Carcinoma","volume":"95","author":"Marti\u0301n-Saiz","year":"2023","journal-title":"Anal Chem."},{"issue":"1","key":"2026092909092380400_cit28","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1038\/s41467-022-28524-0","article-title":"Proteomic\nAnalysis of Archival Breast\nCancer Clinical Specimens Identifies Biological Subtypes with Distinct\nSurvival Outcomes","volume":"13","author":"Asleh","year":"2022","journal-title":"Nat. Commun."},{"key":"2026092909092380400_cit29","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.1007\/s10337-019-03769-y","article-title":"Paraffin-Embedded Tissue as a Novel Matrix in Metabolomics\nStudy: Optimization of Metabolite Extraction Method","volume":"82","author":"Buszewska-Forajta","year":"2019","journal-title":"Chromatographia"},{"issue":"1","key":"2026092909092380400_cit30","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.ccell.2020.09.004","article-title":"Immunometabolic\nInterplay in the Tumor Microenvironment","volume":"39","author":"Kaymak","year":"2021","journal-title":"Cancer\nCell"},{"key":"2026092909092380400_cit31","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1186\/s12934-015-0383-5","article-title":"Three-Dimensional Tumor Spheroids for In Vitro Analysis\nof Bacteria as Gene Delivery Vectors in Tumor Therapy","volume":"14","author":"Osswald","year":"2015","journal-title":"Microb Cell Fact"},{"key":"2026092909092380400_cit32","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1007\/978-3-540-89615-9_13","article-title":"Adenosine Receptors in Wound Healing,\nFibrosis and Angiogenesis","volume":"193","author":"Feoktistov","year":"2009","journal-title":"Handb Exp Pharmacol"},{"issue":"1","key":"2026092909092380400_cit33","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1186\/s12943-024-02193-7","article-title":"Metabolic\nTargeting of Regulatory T Cells in Oral Squamous Cell Carcinoma:\nNew Horizons in Immunotherapy","volume":"23","author":"Gan","year":"2024","journal-title":"Mol. Cancer"},{"issue":"6","key":"2026092909092380400_cit34","doi-asserted-by":"publisher","first-page":"503","DOI":"10.3109\/10409238.2015.1087960","article-title":"RNA Polymerase II Transcriptional\nFidelity Control and Its Functional\nInterplay with DNA Modifications. Crit","volume":"50","author":"Xu","year":"2015","journal-title":"Rev.\nBiochem Mol. Biol."},{"issue":"6","key":"2026092909092380400_cit35","doi-asserted-by":"publisher","first-page":"1901","DOI":"10.1083\/jcb.201802117","article-title":"Epigenetics\nand Epitranscriptomics\nin Temporal Patterning of Cortical Neural Progenitor Competence","volume":"217","author":"Yoon","year":"2018","journal-title":"J. Cell Biol."},{"issue":"8","key":"2026092909092380400_cit36","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1038\/nchembio.1848","article-title":"5-Formylcytosine\nCan. Be a Stable DNA Modification in Mammals","volume":"11","author":"Bachman","year":"2015","journal-title":"Nat. Chem. Biol."},{"issue":"1","key":"2026092909092380400_cit37","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1038\/nsmb.2936","article-title":"5-Formylcytosine Alters the Structure of the DNA Double\nHelix","volume":"22","author":"Raiber","year":"2015","journal-title":"Nat. Struct Mol. Biol."},{"issue":"8","key":"2026092909092380400_cit38","doi-asserted-by":"publisher","DOI":"10.1002\/bies.202300166","article-title":"Interplay between\nAltered Metabolism and DNA Damage\nand Repair in Ovarian Cancer","volume":"46","author":"Uboveja","year":"2024","journal-title":"BioEssays"},{"key":"2026092909092380400_cit39","doi-asserted-by":"publisher","first-page":"46176","DOI":"10.1038\/srep46176","article-title":"Three Serum Metabolite\nSignatures for Diagnosing Low-Grade\nand High-Grade Bladder Cancer","volume":"7","author":"Tan","year":"2017","journal-title":"Sci. Rep"},{"key":"2026092909092380400_cit40","doi-asserted-by":"publisher","first-page":"956","DOI":"10.1002\/tox.21970","article-title":"Modified nucleosides\nas biomarkers for early cancer diagnose in exposed populations","volume":"30","author":"Seidel","year":"2015","journal-title":"Environ. Toxicol."},{"issue":"8","key":"2026092909092380400_cit41","doi-asserted-by":"publisher","first-page":"2127","DOI":"10.1158\/0008-5472.CAN-17-2649","article-title":"Spatial\nHeterogeneity and Evolutionary Dynamics Modulate Time to Recurrence\nin Continuous and Adaptive Cancer Therapies","volume":"78","author":"Gallaher","year":"2018","journal-title":"Cancer Res."},{"issue":"7","key":"2026092909092380400_cit42","doi-asserted-by":"publisher","first-page":"6126","DOI":"10.1021\/acsomega.2c06659","article-title":"Mapping Spatiotemporal\nHeterogeneity in Tumor Profiles by Integrating High-Throughput Imaging\nand Omics Analysis","volume":"8","author":"Patkulkar","year":"2023","journal-title":"ACS Omega"},{"key":"2026092909092380400_cit43","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1186\/1756-0500-3-239","article-title":"Comparative Analysis\nof Four Methods to Extract DNA\nfrom Paraffin-Embedded Tissues: Effect on Downstream Molecular Applications","volume":"3","author":"Huijsmans","year":"2010","journal-title":"BMC Res. Notes"},{"key":"2026092909092380400_cit44","doi-asserted-by":"publisher","first-page":"734134","DOI":"10.3389\/fonc.2021.734134","article-title":"Targeted\nNext-Generation Sequencing\nof Cancer-Related Genes in a Norwegian Patient Cohort With Head and\nNeck Squamous Cell Carcinoma Reveals Novel Actionable Mutations and\nCorrelations With Pathological Parameters","volume":"11","author":"Dongre","year":"2021","journal-title":"Front\nOncol"}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c00978","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/38\/27906\/68547983\/acs.analchem.6c00978.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/38\/27906\/68547983\/acs.analchem.6c00978.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:43:13Z","timestamp":1790689393000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/38\/27906\/5432231\/Integrating-UHPLC-MS-and-MALDI-MSI-for-Spatial"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,17]]},"references-count":44,"journal-issue":{"issue":"38","published-online":{"date-parts":[[2026,9,17]]},"published-print":{"date-parts":[[2026,9,29]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c00978","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,17]]}}}