{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T20:49:47Z","timestamp":1788554987028,"version":"build-2803163510"},"reference-count":35,"publisher":"American Chemical Society (ACS)","issue":"30","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32401107"],"award-info":[{"award-number":["32401107"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82502420"],"award-info":[{"award-number":["82502420"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fusobacterium nucleatum (F. nucleatum), an opportunistic oral anaerobic pathogen, is closely associated with various oral disorders and colorectal cancer (CRC). Current detection methods for this bacterium suffer from technical complexity and labor intensiveness. In this study, we engineered TPAI-YQPVPE, a peptidic fluorescent probe, by conjugating an aggregation-induced emission (AIE) fluorophore (TPAI) with the F. nucleatum-targeting peptide YQPVPE. This probe binds specifically to FomA, a major outer membrane protein of F. nucleatum, enabling its selective accumulation and robust fluorescence emission, even in complex biological environments. Its specificity and detection were validated both in vitro and in vivo in an F. nucleatum infection model, where the probe achieved precise localization at F. nucleatum inoculation foci and exhibited excellent biocompatibility. This AIE-based peptidic probe established a specific and noninvasive detection platform for F. nucleatum, which might represent a promising tool for the early screening and diagnosis of F. nucleatum-associated CRC.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c01904","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T10:29:42Z","timestamp":1784716182000},"page":"22215-22225","source":"Crossref","is-referenced-by-count":2,"title":["Fluorescent\nPeptide Probe for Specific Detection and\nIn Vivo Imaging of Fusobacterium Nucleatum"],"prefix":"10.1021","volume":"98","author":[{"given":"Hang","family":"Wu","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital, Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang Province, P. R. China, 325035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Buyue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nankai University , , ,","place":["Tianjin, P. R. China, 300071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziqiang","family":"Lu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang Province, P. R. China, 325035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Wen","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang Province, P. R. China, 325035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghao","family":"Shen","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang Province, P. R. China, 325035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boya","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang Province, P. R. China, 325035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junchao","family":"Xiong","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang Province, P. R. China, 325035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengyan","family":"Xu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang Province, P. R. China, 325035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2967-6920","authenticated-orcid":true,"given":"Zhimou","family":"Yang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang Province, P. R. China, 325035"]},{"name":"Nankai University , , ,","place":["Tianjin, P. R. China, 300071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0485-7020","authenticated-orcid":true,"given":"Jiali","family":"Chen","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang Province, P. R. 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Animalis Comes to the Spotlight in Oral\nDiseases","volume":"32","author":"Bibek","year":"2024","journal-title":"Cell Host Microbe."},{"key":"2026080408080745100_cit4","doi-asserted-by":"publisher","first-page":"102742","DOI":"10.1016\/j.nantod.2025.102742","article-title":"Oral Delivery\nof Clostridium Butyricum Using Selective\nAntibacterial Lipids for Enhanced Treatment of Fusobacterium Nucleatum-Associated Intestinal Diseases","volume":"62","author":"Zhao","year":"2025","journal-title":"Nano Today"},{"issue":"1","key":"2026080408080745100_cit5","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1136\/gut.2009.191320","article-title":"Acute Appendicitis Is Characterised by Local\nInvasion with Fusobacterium Nucleatum\/Necrophorum","volume":"60","author":"Swidsinski","year":"2009","journal-title":"Gut"},{"issue":"8007","key":"2026080408080745100_cit6","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1038\/s41586-024-07182-w","article-title":"A Distinct Fusobacterium Nucleatum Clade Dominates\nthe Colorectal Cancer Niche","volume":"628","author":"Zepeda-Rivera","year":"2024","journal-title":"Nature"},{"issue":"3","key":"2026080408080745100_cit7","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1016\/j.ccell.2025.02.023","article-title":"Fusobacterium Nucleatum Facilitates Anti-Pd-1 Therapy\nin Microsatellite Stable Colorectal Cancer","volume":"43","author":"Wang","year":"2025","journal-title":"Cancer Cell"},{"key":"2026080408080745100_cit8","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1038\/s41551-019-0423-2","article-title":"Phage-Guided\nModulation of the Gut\nMicrobiota of Mouse Models of Colorectal Cancer Augments Their Responses\nto Chemotherapy","volume":"3","author":"Zheng","year":"2019","journal-title":"Nat. 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Microbiol."},{"issue":"45","key":"2026080408080745100_cit27","doi-asserted-by":"publisher","first-page":"2306281","DOI":"10.1002\/adma.202306281","article-title":"Antibacterial Fusobacterium\nNucleatum-Mimicking Nanomedicine\nto Selectively Eliminate Tumor-Colonized Bacteria and Enhance Immunotherapy\nagainst Colorectal Cancer","volume":"35","author":"Chen","year":"2023","journal-title":"Adv. Mater."},{"key":"2026080408080745100_cit28","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ads3844","article-title":"Self-Assembling\nPeptide\nwith Dual Function of Cell Penetration and Antibacterial as a Nano\nWeapon to Combat Intracellular Bacteria","volume":"11","author":"Zhu","year":"2025","journal-title":"Sci.\nAdv."},{"issue":"18","key":"2026080408080745100_cit29","doi-asserted-by":"publisher","first-page":"1740","DOI":"10.1039\/b105159h","article-title":"Aggregation-Induced Emission of 1-Methyl-1,2,3,4,5-Pentaphenylsilole","author":"Luo","year":"2001","journal-title":"Chem. 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Microbiol."},{"key":"2026080408080745100_cit33","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1080\/19490976.2019.1591137","article-title":"Probiotics to Prevent Staphylococcus Aureus Disease?","volume":"11","author":"Piewngam","year":"2020","journal-title":"Gut\nMicrobes"},{"issue":"13","key":"2026080408080745100_cit34","doi-asserted-by":"publisher","first-page":"9188","DOI":"10.1021\/acs.analchem.0c01541","article-title":"Chalcogenides-Based Tubular Micromotors in Fluorescent\nAssays","volume":"92","author":"de la Asuncio\u0301n-Nadal","year":"2020","journal-title":"Anal. Chem."},{"issue":"1","key":"2026080408080745100_cit35","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1002\/anie.201507031","article-title":"Highly Efficient Far\nRed\/near-Infrared Solid Fluorophores: Aggregation-Induced Emission,\nIntramolecular Charge Transfer, Twisted Molecular Conformation, and\nBioimaging Applications","volume":"55","author":"Lu","year":"2016","journal-title":"Angew. 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