{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:18:58Z","timestamp":1788434338891,"version":"build-2803163510"},"reference-count":32,"publisher":"American Chemical Society (ACS)","issue":"34","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21305053"],"award-info":[{"award-number":["21305053"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004030","name":"Jiangsu Normal University","doi-asserted-by":"publisher","award":["24XFRS044"],"award-info":[{"award-number":["24XFRS044"]}],"id":[{"id":"10.13039\/501100004030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Early detection and deactivation of Staphylococcus aureus (S. aureus) are of great importance to prevent pathogen-induced bacterial infectious diseases. Here, we initially synthesized stimuli-responsive DNA microcapsule coencapsulating gold nanoclusters (Au NCs) and silver nanoparticles (Ag NPs) (Au\/Ag\/DNA microcapsule) and, using it, developed a sensing platform for the detection and elimination of S. aureus. In this system, Ag NPs were used as inactivators, while Au NCs acted as fluorescence (FL) probes. Single-stranded DNA 1 (S1), single-stranded DNA 2 (S2), and aptamer (Apt) were hybridized on the surface of the microcapsule to form a DNA shell. In the presence of S. aureus, the Apt specifically bound to it, triggering the unlocking of the DNA microcapsule and the release of Au NCs and Ag NPs. The amount of Au NCs, which depends on the concentration of S. aureus, could be monitored by fluorescence (FL) spectroscopy and enable quantitative detection of S. aureus over a linear range of 101\u2013107 CFU\/mL. Meanwhile, the released Ag NPs could achieve in situ elimination of S. aureus. This study demonstrated promising application potential in the antibacterial treatment of skin infections and provided valuable insights into the integration of detection and deactivation strategies for S. aureus.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c01924","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:01:53Z","timestamp":1786986113000},"page":"24888-24897","source":"Crossref","is-referenced-by-count":0,"title":["An Au NC- and\nAg NP-Loaded DNA Microcapsule-Based\nSystem for the Detection and Elimination of\n                    <i>Staphylococcus aureus<\/i>"],"prefix":"10.1021","volume":"98","author":[{"given":"Kaiqiu","family":"Li","sequence":"first","affiliation":[{"name":"Jiangsu Normal University , , ,","place":["Xuzhou, P. R. China, 221116"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"Zhejiang Shuren University , , ,","place":["Hangzhou, PR China, 310015"]},{"name":"Zhejiang Chinese Medical University , , ,","place":["Hangzhou, PR China, 310053"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yani","family":"Chen","sequence":"additional","affiliation":[{"name":"Jiangsu Normal University , , ,","place":["Xuzhou, P. R. China, 221116"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Normal University , , ,","place":["Xuzhou, P. R. China, 221116"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Dong","sequence":"additional","affiliation":[{"name":"Zhejiang Shuren University , , ,","place":["Hangzhou, PR China, 310015"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5126-9304","authenticated-orcid":true,"given":"Xiangmin","family":"Miao","sequence":"additional","affiliation":[{"name":"Jiangsu Normal University , , ,","place":["Xuzhou, P. R. 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Mater."},{"key":"2026090306471013000_cit4","doi-asserted-by":"publisher","first-page":"5128","DOI":"10.1021\/jacs.3c09033","article-title":"Mechanism of Action and Design of\nPotent Antibacterial Block Copolymer Nanoparticles","volume":"146","author":"Parkin","year":"2024","journal-title":"J. Am. Chem. Soc."},{"key":"2026090306471013000_cit5","doi-asserted-by":"publisher","first-page":"124335","DOI":"10.1016\/j.jhazmat.2020.124335","article-title":"Detection\nand Distribution of Vbnc\/viable Pathogenic Bacteria in Full-scale\nDrinking Water Treatment Plants","volume":"406","author":"Guo","year":"2021","journal-title":"J. Hazard.\nMater."},{"key":"2026090306471013000_cit6","doi-asserted-by":"publisher","first-page":"9237","DOI":"10.1021\/acs.analchem.3c00743","article-title":"A Versatile pdRu Bimetallic Nanoenzyme-integrated\nEnzyme-linked Immunosorbent Assay for Highly Sensitive Escherichia coli O157:H7 Detection","volume":"95","author":"Wang","year":"2023","journal-title":"Anal. 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Mater.\nEng."},{"key":"2026090306471013000_cit30","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1007\/s11259-025-10900-y","article-title":"Antibacterial,\nAnti-biofilm and Anti-virulence\nActivity of Biosynthesized Silver Nanoparticles Against Drug-resistant Staphylococcus aureus","volume":"49","author":"Singh","year":"2025","journal-title":"Vet. Res. Commun."},{"key":"2026090306471013000_cit31","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.3390\/microorganisms11061539","article-title":"Biosynthesis of\nSilver Nanoparticles from Duchesnea\nIndica Extracts using Different Solvents and Their Antibacterial Activity","volume":"11","author":"Kim","year":"2023","journal-title":"Microorganisms"},{"key":"2026090306471013000_cit32","doi-asserted-by":"publisher","first-page":"1746","DOI":"10.3390\/foods12091746","article-title":"Silver Nanoparticle Synthesis by\nRumex Vesicarius Extract and Its Applicability Against Foodborne Pathogens","volume":"12","author":"Elsebaie","year":"2023","journal-title":"Foods"}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c01924","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/34\/24888\/66650824\/acs.analchem.6c01924.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/34\/24888\/66650824\/acs.analchem.6c01924.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:47:20Z","timestamp":1788432440000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/34\/24888\/5262235\/An-Au-NC-and-Ag-NP-Loaded-DNA-Microcapsule-Based"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":32,"journal-issue":{"issue":"34","published-online":{"date-parts":[[2026,8,17]]},"published-print":{"date-parts":[[2026,9,1]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c01924","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]}}}