{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T16:16:20Z","timestamp":1789488980854,"version":"build-2803163510"},"reference-count":38,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82402433"],"award-info":[{"award-number":["82402433"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Demonstration Center for Experimental Basic Medical Science Education, Xuzhou Medical University","award":["2024BMS19"],"award-info":[{"award-number":["2024BMS19"]}]},{"name":"National Demonstration Center for Experimental Basic Medical Science Education, Xuzhou Medical University","award":["2025BMS13"],"award-info":[{"award-number":["2025BMS13"]}]},{"name":"National Demonstration Center for Experimental Basic Medical Science Education, Xuzhou Medical University","award":["2025BMS19"],"award-info":[{"award-number":["2025BMS19"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cytoplasmic viscosity dynamically varies when cells respond to physiological, pathological, or external stimuli. Precise analysis of these minimal fluctuations requires high sensitivity for real-time, high-fidelity monitoring. Herein, we developed an amino-functionalized nanopipette as a highly sensitive nanosensor for real-time dynamic monitoring of cytoplasmic viscosity in DU-145 human prostate cancer cells under both hypertonic and hypotonic conditions. Amino functionalization significantly enhanced the nanopipette\u2019s sensitivity to viscosity. Within a scanning window of \u22121.0 V to +1.0 V, it yielded a 189.56-fold increase at +1.0 V and up to a 17.96-fold improvement at their respective optimal applied potentials. This enhancement is primarily attributed to the increased interfacial charge density introduced by amino groups, which strengthens both the electrical double-layer effect and electroosmotic flow. Notably, the nanosensor exhibits high sensitivity, excellent selectivity, and good reversibility in both voltammetry and chronoamperometry, while maintaining its viscosity-response performance across a wide range of electrolyte concentrations and pH levels. This work not only provides a powerful tool for dynamic monitoring of cytoplasmic viscosity but also presents a strategy to enhance viscosity sensitivity through inner-wall chemical modification, which regulates nanoscale mass transport.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c01931","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:10:08Z","timestamp":1788401408000},"page":"26441-26450","source":"Crossref","is-referenced-by-count":0,"title":["Amino Functionalization\nSignificantly Boosts Nanopipette\nSensitivity to Viscosity"],"prefix":"10.1021","volume":"98","author":[{"given":"Yuchen","family":"Wu","sequence":"first","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueqian","family":"Zhu","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xufan","family":"Wang","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijing","family":"Liu","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinqian","family":"Xu","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxuan","family":"Ma","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyi","family":"Peng","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yipei","family":"Xue","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoxin","family":"Bai","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianing","family":"Xing","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen","family":"Sun","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Liu","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Zhong","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , , ,","place":["Xuzhou, Jiangsu, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0936-1238","authenticated-orcid":true,"given":"Guochang","family":"Liu","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]},{"name":"Xuzhou Medical University , , ,","place":["Xuzhou, China, 221004"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"issue":"1","key":"2026091511074239000_cit1","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1021\/acssensors.4c02699","article-title":"Sulfone-Embedded\nNIR Fluorophore with Large Stokes Shift for Monitoring Viscosity Changes\nduring NAFLD-Induced Ferroptosis","volume":"10","author":"Chen","year":"2025","journal-title":"ACS Sens"},{"issue":"42","key":"2026091511074239000_cit2","doi-asserted-by":"publisher","first-page":"9642","DOI":"10.1039\/D0TB01146K","article-title":"A Minireview 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Cell Biol"},{"issue":"12","key":"2026091511074239000_cit36","doi-asserted-by":"publisher","first-page":"3532","DOI":"10.3390\/cells10123532","article-title":"Stability of Intracellular Protein Concentration under\nExtreme Osmotic Challenge","volume":"10","author":"Hollembeak","year":"2021","journal-title":"Cells"},{"issue":"47","key":"2026091511074239000_cit37","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2103228118","article-title":"Passive Coupling of Membrane Tension\nand Cell Volume during Active Response of Cells to Osmosis","volume":"118","author":"Roffay","year":"2021","journal-title":"Proc. Natl. Acad. Sci. U. S. 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