{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T12:49:06Z","timestamp":1785847746640,"version":"3.56.0"},"reference-count":36,"publisher":"American Chemical Society (ACS)","issue":"30","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22304074"],"award-info":[{"award-number":["22304074"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2022JJ30484"],"award-info":[{"award-number":["2022JJ30484"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2022JJ40358"],"award-info":[{"award-number":["2022JJ40358"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2025JJ50072"],"award-info":[{"award-number":["2025JJ50072"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mercury ions (Hg2+) are highly toxic heavy metals that are widely distributed in the environment and pose a serious threat to both ecosystems and human health. After entering the body, mercury preferentially accumulates in the liver and kidneys, underscoring the importance of early detection of acute and chronic hepatorenal injuries. Although fluorescent probes have been widely explored for disease diagnosis, most existing systems cannot achieve simultaneous in vivo imaging of the liver and kidneys, nor can they monitor multiple pathological parameters during hepatorenal injury, thus limiting their utility in evaluating Hg2+-induced multiorgan damage. In this study, we developed a multifunctional fluorescent probe, VPO, with high sensitivity and good biocompatibility, which can be used to monitor polarity, viscosity, and ONOO\u2013 levels in cells. Using VPO, we observed, for the first time, a significant increase in ONOO\u2013 during pyroptosis, accompanied by an elevated mitochondrial viscosity and reduced polarity. In addition, VPO successfully enabled the real-time visual monitoring of these changes in mercury-exposed cells, zebrafish, and mice. Moreover, its multiresponsive property enabled the evaluation of the therapeutic efficacy of N-acetylcysteine (NAC) against Hg2+-induced hepatorenal injury. NAC alleviated hepatic and renal mitochondrial oxidative stress, as well as pyroptosis-related inflammatory signaling, by reversing Hg2+-induced biomarker abnormalities. In summary, these findings demonstrate that VPO provides a comprehensive platform for the early diagnosis, therapeutic evaluation, and mechanistic investigation of mercury poisoning.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c02189","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T09:11:45Z","timestamp":1784711505000},"page":"22226-22234","source":"Crossref","is-referenced-by-count":0,"title":["A Multi-Analyte-Responsive, Multi-Organ-Imaging, Dual-NIR-Emissive\nFluorescent Probe for Visualizing Pyroptosis-Mediated Hg2+-Induced Hepatorenal Dysfunction"],"prefix":"10.1021","volume":"98","author":[{"given":"Zibo","family":"Zhai","sequence":"first","affiliation":[{"name":"University of South China , , , ,","place":["Hengyang, Hunan, China, 421001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Tao","sequence":"additional","affiliation":[{"name":"University of South China , , , ,","place":["Hengyang, Hunan, China, 421001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Huang","sequence":"additional","affiliation":[{"name":"University of South China , , ,","place":["Hengyang, China, 421002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailin","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of South China , , , ,","place":["Hengyang, Hunan, China, 421001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xijian","family":"Wang","sequence":"additional","affiliation":[{"name":"University of South China , , , ,","place":["Hengyang, Hunan, China, 421001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Deng","sequence":"additional","affiliation":[{"name":"University of South China , , , ,","place":["Hengyang, Hunan, China, 421001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiqi","family":"Wen","sequence":"additional","affiliation":[{"name":"University of South China , , , ,","place":["Hengyang, Hunan, China, 421001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Cheng","sequence":"additional","affiliation":[{"name":"University of South China , , , ,","place":["Hengyang, Hunan, China, 421001"]},{"name":"University of South China , , ,","place":["Hengyang, P. R. China, 421002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8206-8783","authenticated-orcid":true,"given":"Longwei","family":"He","sequence":"additional","affiliation":[{"name":"University of South China , , , ,","place":["Hengyang, Hunan, China, 421001"]},{"name":"University of South China , , ,","place":["Hengyang, China, 421002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2289-9284","authenticated-orcid":true,"given":"Songjiao","family":"Li","sequence":"additional","affiliation":[{"name":"University of South China , , , ,","place":["Hengyang, Hunan, China, 421001"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"2026080408081777400_cit1","doi-asserted-by":"publisher","first-page":"113211","DOI":"10.1016\/j.envres.2022.113211","article-title":"Surface\ngrafted silica adsorbent for efficient removal of Hg2+ ions\nfrom contaminated water","volume":"212","author":"Santhamoorthy","year":"2022","journal-title":"Environ. 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Total Environ."},{"key":"2026080408081777400_cit3","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/s12940-022-00838-y","article-title":"Past mercury\nexposure and current symptoms of nervous system dysfunction in adults\nof a First Nation community (Canada)","volume":"21","author":"Philibert","year":"2022","journal-title":"Environ.\nHealth"},{"key":"2026080408081777400_cit4","doi-asserted-by":"publisher","first-page":"113018","DOI":"10.1016\/j.ecoenv.2021.113018","article-title":"Selenium\nalleviates mercury chloride-induced liver injury by regulating mitochondrial\ndynamics to inhibit the crosstalk between energy metabolism disorder\nand NF-\u03baB\/NLRP3 inflammasome-mediated inflammation","volume":"228","author":"Gao","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"2026080408081777400_cit5","doi-asserted-by":"publisher","first-page":"129673","DOI":"10.1016\/j.chemosphere.2021.129673","article-title":"Evaluation of dietary\nselenium, vitamin C and E as the multi-antioxidants on the methylmercury\nintoxicated mice based on mercury bioaccumulation, antioxidant enzyme\nactivity, lipid peroxidation and mitochondrial oxidative stress","volume":"273","author":"Moniruzzaman","year":"2021","journal-title":"Chemosphere"},{"key":"2026080408081777400_cit6","doi-asserted-by":"publisher","first-page":"338941","DOI":"10.1016\/j.aca.2021.338941","article-title":"Investigation on selenium\nand mercury interactions and the distribution patterns in mice organs\nwith LA-ICP-MS imaging","volume":"1182","author":"Liu","year":"2021","journal-title":"Anal. Chim. Acta"},{"key":"2026080408081777400_cit7","doi-asserted-by":"publisher","first-page":"799","DOI":"10.1021\/acs.analchem.5c05931","article-title":"Label-Free\nSERS Strategy for Simultaneous Detection of Triplex Renal Biomarkers\nin Serum toward Precise Renal Function Evaluation","volume":"98","author":"Li","year":"2026","journal-title":"Anal. Chem."},{"key":"2026080408081777400_cit8","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.procbio.2022.01.010","article-title":"Current advancement\nin biosensing techniques for determination of alanine aminotransferase\nand aspartate aminotransferase a mini review","volume":"114","author":"Yadav","year":"2022","journal-title":"Process Biochem."},{"key":"2026080408081777400_cit9","doi-asserted-by":"publisher","first-page":"1338","DOI":"10.1021\/acs.chemrev.7b00568","article-title":"Biochemistry\nof peroxynitrite and protein tyrosine nitration","volume":"118","author":"Ferrer-Sueta","year":"2018","journal-title":"Chem. Rev."},{"key":"2026080408081777400_cit10","doi-asserted-by":"publisher","first-page":"102900","DOI":"10.1016\/j.nantod.2025.102900","article-title":"Electronic\ncloud-regulation strategy enabling rapid and sensitive near-infrared\ndetection of ONOO\u2013 for dynamic monitoring of ferroptosis-mediated\ndrug-induced liver injury","volume":"66","author":"Li","year":"2026","journal-title":"Nano Today"},{"key":"2026080408081777400_cit11","doi-asserted-by":"publisher","first-page":"342611","DOI":"10.1016\/j.aca.2024.342611","article-title":"A novel ESIPT fluorescent probe for\nearly detection and assessment\nof ferroptosis-mediated acute kidney injury via peroxynitrite fluctuation","volume":"1308","author":"Chen","year":"2024","journal-title":"Anal. Chim. Acta"},{"key":"2026080408081777400_cit12","doi-asserted-by":"publisher","first-page":"1738","DOI":"10.1021\/acs.chemrev.3c00573","article-title":"Design and\napplication of fluorescent probes to detect cellular physical microenvironments","volume":"124","author":"Ma","year":"2024","journal-title":"Chem. Rev."},{"key":"2026080408081777400_cit13","doi-asserted-by":"publisher","first-page":"9642","DOI":"10.1039\/D0TB01146K","article-title":"A minireview of viscosity-sensitive\nfluorescent probes: design and biological applications","volume":"8","author":"Ma","year":"2020","journal-title":"J. Mater. Chem. B"},{"key":"2026080408081777400_cit14","doi-asserted-by":"publisher","first-page":"1856","DOI":"10.1007\/s00284-021-02454-4","article-title":"Comparative\nstudy of cytotoxicity, DNA damage and oxidative\nstress induced by heavy metals Cd(II), Hg(II) and Cr(III) in yeast","volume":"78","author":"Wang","year":"2021","journal-title":"Curr. Microbiol."},{"key":"2026080408081777400_cit15","doi-asserted-by":"publisher","first-page":"2749","DOI":"10.1039\/C9TB00043G","article-title":"Immobilizable fluorescent\nprobes for monitoring the mitochondria microenvironment: a next step\nfrom the classic","volume":"7","author":"Zhang","year":"2019","journal-title":"J. Mater. Chem. B"},{"key":"2026080408081777400_cit16","doi-asserted-by":"publisher","first-page":"125216","DOI":"10.1016\/j.talanta.2023.125216","article-title":"Engineering an activatable fluorescent probe for studying ONOO\u2013 in pyroptotic process","volume":"267","author":"Gong","year":"2024","journal-title":"Talanta"},{"key":"2026080408081777400_cit17","doi-asserted-by":"publisher","first-page":"138498","DOI":"10.1016\/j.snb.2025.138498","article-title":"Engineering neutral\nprobes for investigating the role of ROS in the formation of G4s by\nfluorescence imaging in tumor cells","volume":"444","author":"Chen","year":"2025","journal-title":"Sens. Actuators\nB Chem."},{"key":"2026080408081777400_cit18","doi-asserted-by":"publisher","first-page":"118675","DOI":"10.1016\/j.trac.2026.118675","article-title":"Chemical design of\norganelle-targeting molecular rotors for intracellular\nviscosity imaging","volume":"197","author":"Han","year":"2026","journal-title":"TrAC, Trends Anal. Chem."},{"key":"2026080408081777400_cit19","doi-asserted-by":"publisher","first-page":"213582","DOI":"10.1016\/j.ccr.2020.213582","article-title":"Recent progresses in\nfluorescent probes for detection\nof polarity","volume":"427","author":"Xiao","year":"2021","journal-title":"Coord. Chem. Rev."},{"key":"2026080408081777400_cit20","doi-asserted-by":"publisher","first-page":"13629","DOI":"10.1039\/D4CC03452J","article-title":"Recent progresses\nin organelle-targeting fluorescent probes for the detection of peroxynitrite","volume":"60","author":"Li","year":"2024","journal-title":"Chem. Commun."},{"key":"2026080408081777400_cit21","doi-asserted-by":"publisher","first-page":"2302466","DOI":"10.1002\/adhm.202302466","article-title":"De Novo Design\nof Near-Infrared Fluorescent\nAgents Activated by Peroxynitrite and Glutathione-Responsive Imaging\nfor Diabetic Liver Disease","volume":"13","author":"Jiang","year":"2024","journal-title":"Adv. Healthcare\nMater."},{"key":"2026080408081777400_cit22","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202515602","article-title":"Dual-Responsive\nFluorescent Probes: Advances in Biosensing, Diagnosis and Therapy","volume":"36","author":"Liu","year":"2026","journal-title":"Adv. Funct. Mater."},{"key":"2026080408081777400_cit23","doi-asserted-by":"publisher","first-page":"139556","DOI":"10.1016\/j.snb.2026.139556","article-title":"Multifunctional\nfluorescence probe for detecting viscosity, ONOO\u2013 and polarity in vitro and in vivo with three channels","volume":"453","author":"Shang","year":"2026","journal-title":"Sens. Actuators B Chem."},{"key":"2026080408081777400_cit24","doi-asserted-by":"publisher","first-page":"10155","DOI":"10.1021\/acs.analchem.2c05733","article-title":"Mitochondria-Targeting\nMultifunctional Fluorescent Probe toward Polarity, Viscosity, and\nONOO\u2013 and Cell Imaging","volume":"95","author":"Huang","year":"2023","journal-title":"Anal.\nChem."},{"key":"2026080408081777400_cit25","doi-asserted-by":"publisher","first-page":"116233","DOI":"10.1016\/j.bios.2024.116233","article-title":"Multifunctional\nfluorescence probe\nfor simultaneous detection of viscosity, polarity, and ONOO\u2013 and its bioimaging in vitro and vivo","volume":"254","author":"Li","year":"2024","journal-title":"Biosens.\nBioelectron."},{"key":"2026080408081777400_cit26","doi-asserted-by":"publisher","first-page":"5780","DOI":"10.1021\/acs.analchem.3c00142","article-title":"Multifunctional\nfluorescent probe for simultaneous detection of ONOO\u2013, viscosity, and polarity and its application in ferroptosis and\ncancer models","volume":"95","author":"Fan","year":"2023","journal-title":"Anal. Chem."},{"key":"2026080408081777400_cit27","doi-asserted-by":"publisher","first-page":"130612","DOI":"10.1016\/j.jhazmat.2022.130612","article-title":"Monitoring Hg2+ and MeHg+ poisoning in living\nbody with an activatable near-infrared II fluorescence probe","volume":"445","author":"Ye","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"2026080408081777400_cit28","doi-asserted-by":"publisher","first-page":"136059","DOI":"10.1016\/j.jhazmat.2024.136059","article-title":"Shortening\nthe early diagnostic window of Hg2+-induced liver injury\nwith a H2O2-activated fluorescence\/afterglow\nimaging assay","volume":"480","author":"Li","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"2026080408081777400_cit29","doi-asserted-by":"publisher","first-page":"139462","DOI":"10.1016\/j.jhazmat.2025.139462","article-title":"Real-time tracking and early diagnosis of mercury(II)\nexposure via\na novel NIR-based fluorescent probe based on thiahemicyanine dye","volume":"496","author":"Liu","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"2026080408081777400_cit30","doi-asserted-by":"publisher","first-page":"1446","DOI":"10.1021\/acs.analchem.4c06622","article-title":"Mercury(II)-Triggered\nTargeted and NIR-II Fluorescence\/Photoacoustic\nImaging Probe for High-Sensitivity Early Diagnosis and Evaluating\nDrug against Acute Liver and Kidney Injury","volume":"97","author":"Li","year":"2025","journal-title":"Anal. Chem."},{"key":"2026080408081777400_cit31","doi-asserted-by":"publisher","first-page":"139091","DOI":"10.1016\/j.snb.2025.139091","article-title":"Long-emission\nfluorescent probe for\nsulfite detection: In vivo imaging in acute liver\nand renal injury models","volume":"449","author":"Zhang","year":"2026","journal-title":"Sens. Actuators B Chem."},{"key":"2026080408081777400_cit32","doi-asserted-by":"publisher","first-page":"10004","DOI":"10.1039\/D4TB01446D","article-title":"Imaging of\nONOO\u2013 fluctuations during drug-induced liver\/kidney\ninjury in vitro and in vivo via a dicyanoisophorone-based\nNIR fluorescent probe\nwith a large Stokes shift","volume":"12","author":"Kong","year":"2024","journal-title":"J. Mater. Chem. B"},{"key":"2026080408081777400_cit33","doi-asserted-by":"publisher","first-page":"123647","DOI":"10.1016\/j.talanta.2022.123647","article-title":"A new NIR\nemission mitochondrial targetable fluorescent probe and its application\nin detecting viscosity changes in mouse liver and kidney injury","volume":"249","author":"Tang","year":"2022","journal-title":"Talanta"},{"key":"2026080408081777400_cit34","doi-asserted-by":"publisher","first-page":"117881","DOI":"10.1016\/j.bios.2025.117881","article-title":"A near-infrared ratiometric\noptical sensor for real-time monitoring hydroxyl radical levels in\nferroptosis-mediated Parkinson\u2019s disease","volume":"289","author":"Deng","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"2026080408081777400_cit35","doi-asserted-by":"publisher","first-page":"2998","DOI":"10.1021\/acs.analchem.4c06015","article-title":"A triple-responsive\nand dual-NIR emissive fluorescence\nprobe for precise cancer imaging and therapy by activating pyroptosis\npathway","volume":"97","author":"Deng","year":"2025","journal-title":"Anal. Chem."},{"key":"2026080408081777400_cit36","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1186\/s12944-020-01274-y","article-title":"Long term\nN-acetylcysteine administration rescues liver steatosis via endoplasmic\nreticulum stress with unfolded protein response in mice","volume":"19","author":"Tsai","year":"2020","journal-title":"Lipids Health Dis."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c02189","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/30\/22226\/66116195\/acs.analchem.6c02189.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/30\/22226\/66116195\/acs.analchem.6c02189.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T12:21:52Z","timestamp":1785846112000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/30\/22226\/5226284\/A-Multi-Analyte-Responsive-Multi-Organ-Imaging"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,22]]},"references-count":36,"journal-issue":{"issue":"30","published-online":{"date-parts":[[2026,7,22]]},"published-print":{"date-parts":[[2026,8,4]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c02189","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,22]]}}}