{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T13:04:34Z","timestamp":1785243874839,"version":"3.55.0"},"reference-count":54,"publisher":"American Chemical Society (ACS)","issue":"29","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100003347","name":"Fudan University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22377016"],"award-info":[{"award-number":["22377016"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Noncommunicable Chronic Diseases-National Science and Technology Major Program","award":["2023ZD0500400"],"award-info":[{"award-number":["2023ZD0500400"]}]},{"DOI":"10.13039\/501100008839","name":"Shanghai Xuhui Central Hospital","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008839","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fund of Fudan University and Cao?ejiang Basic Research Program","award":["24FCA08"],"award-info":[{"award-number":["24FCA08"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Heavy water (D2O) labeling provides a safe and universal approach for probing biosynthesis in mid-infrared (MIR) metabolic imaging. However, current analysis typically simplifies the C\u2013D spectral region into two peaks associated with lipid and protein synthesis, overlooking the broader spectral complexity arising from multiple deuteration sites and chemical environments. Here, we show that in vivo D2O labeling produces multiband C\u2013D spectral features spanning 2000\u20132300 cm\u20131, reflecting contributions from distinct d1, d2, and d3 configurations across lipids, proteins, and other biomolecules. To characterize this spectral complexity, we introduce a suite of phasor-based analyses that transform hyperspectral MIR data into a two-dimensional, chemically interpretable representation, enabling model-free dimensionality reduction while preserving spectral mixing relationships. Within this framework, phasor coloring enables rapid, segmentation-free visualization of multiscale metabolic heterogeneity, whereas phasor-ratio analysis identifies localized metabolic niches, including Purkinje cells in the cerebellum, intestinal villi, and tumor invasive margins. Phasor-based segmentation results of the mouse brain are quantitatively compared with the Allen Brain Atlas, showing good agreement with histological architecture. This method presents a rapid and effective way to decipher deuterium-labeled MIR imaging data and visualize the spatial metabolic organization of tissues.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c02256","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T04:25:16Z","timestamp":1783916716000},"page":"21558-21570","source":"Crossref","is-referenced-by-count":0,"title":["Phasor Analysis\nof Multiband Deuterium Mid-Infrared\nImaging Reveals Spatial Metabolic Organization in Tissues"],"prefix":"10.1021","volume":"98","author":[{"given":"Minqian","family":"Wei","sequence":"first","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1797-3690","authenticated-orcid":true,"given":"Zihang","family":"He","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Sun","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9712-5800","authenticated-orcid":true,"given":"Lixue","family":"Shi","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200032"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,13]]},"reference":[{"key":"2026072808071121500_cit1","doi-asserted-by":"publisher","first-page":"8260","DOI":"10.1038\/s41467-023-43917-5","article-title":"Single-cell spatial metabolomics\nwith cell-type specific protein profiling for tissue systems biology","volume":"14","author":"Hu","year":"2023","journal-title":"Nat. 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Metab."},{"key":"2026072808071121500_cit31","doi-asserted-by":"publisher","first-page":"2125","DOI":"10.1021\/acs.analchem.0c03925","article-title":"D2O-Probed\nRaman Microspectroscopy Distinguishes the Metabolic Dynamics of Macromolecules\nin Organellar Anticancer Drug Response","volume":"93","author":"Hekmatara","year":"2021","journal-title":"Anal.\nChem."},{"key":"2026072808071121500_cit32","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1146\/annurev-biophys-052118-115500","article-title":"Raman Imaging of Small Biomolecules","volume":"48","author":"Shen","year":"2019","journal-title":"Annu. Rev. 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Chem."},{"key":"2026072808071121500_cit45","doi-asserted-by":"publisher","first-page":"4115","DOI":"10.1021\/ac500014b","article-title":"Reliable Cell Segmentation Based on Spectral Phasor\nAnalysis of Hyperspectral Stimulated Raman Scattering Imaging Data","volume":"86","author":"Fu","year":"2014","journal-title":"Anal. Chem."},{"key":"2026072808071121500_cit46","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1038\/s41586-025-09616-5","article-title":"Spatial metabolic gradients in the liver and\nsmall intestine","volume":"648","author":"Samarah","year":"2025","journal-title":"Nature"},{"key":"2026072808071121500_cit47","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1111\/j.1471-4159.1977.tb10649.x","article-title":"The [14C]deoxyglucose\nmethod for the measurement of local cerebral glucose utilization:\ntheory, procedure, and normal values in the conscious and anesthetized\nalbino rat","volume":"28","author":"Sokoloff","year":"1977","journal-title":"J. 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Photonics"}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c02256","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/29\/21558\/65884995\/acs.analchem.6c02256.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/29\/21558\/65884995\/acs.analchem.6c02256.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:21:26Z","timestamp":1785241286000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/29\/21558\/5207050\/Phasor-Analysis-of-Multiband-Deuterium-Mid"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,13]]},"references-count":54,"journal-issue":{"issue":"29","published-online":{"date-parts":[[2026,7,13]]},"published-print":{"date-parts":[[2026,7,28]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c02256","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,13]]}}}