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To address these issues, we developed a reusable pH test strip based on the strong adsorption between Congo red and a nylon film. It was prepared by a simple soaking-ultrasonic washing process (optimal washing times is 15) and can be reused at least 100 times. After a single calibration, it maintains a deviation within \u00b10.1 from the pH meter following 11 days of storage in water and within \u00b10.15 after 30 days, demonstrating excellent stability. The response shows low temperature sensitivity in the range of 10.2\u201339.1 \u00b0C and no response to common ions (Na+, K+, Ca+, Mg2+, Cu2+, Fe3+, Zn2+, Pb2+, and Hg2+). Using a smartphone, the Total Huev parameter, and IQA software, the strip enables accurate pH determination of HCl solutions and simulated gastric fluids. Thus, it serves as a low-cost alternative to pH meters for preparing acidic solutions (pH 0.5\u20131.5) and for preliminary screening of the Zollinger\u2013Ellison syndrome, reducing damage to pH electrodes from highly acidic media. Furthermore, the strip identifies four concentrated acid reagents (H2SO4, CH3COOH, HNO3, and HCl) via kinetic response, naked-eye color comparison, or principal component analysis. Future work includes extending this reusable platform to broader pH ranges to mitigate environmental pollution from disposable test strips. The exceptional leaching resistance of the Congo red-nylon film makes it promising for pH imaging.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c02424","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T10:02:11Z","timestamp":1786615331000},"page":"24188-24199","source":"Crossref","is-referenced-by-count":1,"title":["Reusable pH\nTest Strip for Extremely Acidic pH Detection"],"prefix":"10.1021","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0008-4175","authenticated-orcid":true,"given":"Haitao","family":"Sun","sequence":"first","affiliation":[{"name":"Nanjing University , , , ,","place":["Nanjing, Jiangsu, China, 210023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nanjing University , , , ,","place":["Nanjing, Jiangsu, China, 210023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nanjing University , , , ,","place":["Nanjing, Jiangsu, China, 210023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Nanjing University , , , ,","place":["Nanjing, Jiangsu, China, 210023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyu","family":"Lin","sequence":"additional","affiliation":[{"name":"Nanjing University , , , ,","place":["Nanjing, Jiangsu, China, 210023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Liu","sequence":"additional","affiliation":[{"name":"Nanjing University , , , ,","place":["Nanjing, Jiangsu, China, 210023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5312-2384","authenticated-orcid":true,"given":"Chao","family":"Han","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Nanjing, China, 211135"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3480-8900","authenticated-orcid":true,"given":"Jun","family":"Luo","sequence":"additional","affiliation":[{"name":"Nanjing University , , , ,","place":["Nanjing, Jiangsu, China, 210023"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"issue":"21","key":"2026083118452496300_cit1","doi-asserted-by":"publisher","first-page":"39426","DOI":"10.1109\/jsen.2025.3612346","article-title":"High-Performance\npH Sensing for Water Quality Assessment Using RuO2 Nanorods\non FTO Substrates","volume":"25","author":"Naick","year":"2025","journal-title":"IEEE Sens. 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