{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T23:16:16Z","timestamp":1788218176720,"version":"build-2803163510"},"reference-count":23,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22322401"],"award-info":[{"award-number":["22322401"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073816"],"award-info":[{"award-number":["82073816"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2023M730133"],"award-info":[{"award-number":["2023M730133"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005090","name":"Beijing Nova Program","doi-asserted-by":"publisher","award":["20220484055"],"award-info":[{"award-number":["20220484055"]}],"id":[{"id":"10.13039\/501100005090","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Liquid crystal (LC) materials provide a unique sensing platform because their long-range molecular order is highly sensitive to interfacial physicochemical changes. However, most LC-based systems operate at extracellular interfaces, limiting their applicability for intracellular analysis in living cells. Here, we report cell-internalized liquid crystal microcapsules for configuration-based monitoring of local intracellular pH-responsive changes. The sensing platform, denoted M-E7PBA, consists of an E7 liquid crystal core doped with pentyl biphenyl carboxylic acid (PBA) and encapsulated within a polyelectrolyte shell via layer-by-layer self-assembly. Protonation of PBA modulates interfacial anchoring, inducing a reversible radial\u2013bipolar transition of the LC director that can be directly visualized under polarized optical microscopy. This interfacial transduction mechanism enables optical detection of pH variations over a physiologically relevant range (5.8\u20137.8) with a resolution of 0.1 pH units. Importantly, microfluidic control enables efficient and reproducible internalization of the LC microcapsules, allowing intracellular LC optical measurements at the single-cell level. Using this platform, vincristine-induced intracellular acidification in HeLa cells was monitored in real time. These results establish a microfluidic-enabled LC-based analytical strategy for probing intracellular biochemical dynamics in living cells.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c02453","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T18:01:58Z","timestamp":1786644118000},"page":"24270-24277","source":"Crossref","is-referenced-by-count":0,"title":["Internalized\nLiquid Crystal Microcapsules for pH Sensing\nin Living Single Cells"],"prefix":"10.1021","volume":"98","author":[{"given":"Wenjun","family":"Liao","sequence":"first","affiliation":[{"name":"Beijing Technology and Business University , , ,","place":["Beijing, China, 100048"]},{"name":"Tsinghua University , , ,","place":["Beijing, China, 100084"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Technology and Business University , , ,","place":["Beijing, China, 100048"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueting","family":"Xie","sequence":"additional","affiliation":[{"name":"Beijing Technology and Business University , , ,","place":["Beijing, China, 100048"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianzi","family":"Song","sequence":"additional","affiliation":[{"name":"Beijing Technology and Business University , , ,","place":["Beijing, China, 100048"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghua","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Technology and Business University , , ,","place":["Beijing, China, 100048"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyu","family":"Wu","sequence":"additional","affiliation":[{"name":"Beijing Technology and Business University , , ,","place":["Beijing, China, 100048"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1963-2417","authenticated-orcid":true,"given":"Ling","family":"Lin","sequence":"additional","affiliation":[{"name":"Beijing Technology and Business University , , ,","place":["Beijing, China, 100048"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"2026083118453994600_cit1","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1038\/nrc1713","article-title":"The role of disturbed pH dynamics\nand the Na+\/H+ exchanger in metastasis","volume":"5","author":"Cardone","year":"2005","journal-title":"Nat.\nRev. Cancer"},{"key":"2026083118453994600_cit2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2025.163623","article-title":"Photonic interpenetrating\npolymer network intertwined\nwith solid-state cholesteric liquid crystal and cationic polyelectrolyte\nnetworks for a skin-attachable sweat-based glucose biosensor","volume":"515","author":"Adane","year":"2025","journal-title":"Chem. Eng. J."},{"key":"2026083118453994600_cit3","doi-asserted-by":"publisher","first-page":"1201","DOI":"10.1038\/s41556-025-01741-7","article-title":"A pH-dependent\ncondensate switch","volume":"27","author":"Gross","year":"2025","journal-title":"Nat. Cell Biol."},{"key":"2026083118453994600_cit4","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202510253","article-title":"Reshape Intracellular\/Extracellular\npH for Enhanced Nanocatalytic Ferroptosis and cGAS-STING Activation\nInduced Immunotherapy","volume":"22","author":"Gong","year":"2026","journal-title":"Small"},{"key":"2026083118453994600_cit5","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39312-9","article-title":"Intracellular pH dynamics regulates intestinal stem\ncell lineage specification","volume":"14","author":"Liu","year":"2023","journal-title":"Nat. Commun."},{"key":"2026083118453994600_cit6","doi-asserted-by":"publisher","first-page":"1636","DOI":"10.1161\/CIRCRESAHA.125.325386","article-title":"Intracellular pH Regulation in Ventricular\nMyocytes: Implications for Cardiac Health and Disease","volume":"136","author":"Orlowski","year":"2025","journal-title":"Circ. Res."},{"key":"2026083118453994600_cit7","doi-asserted-by":"publisher","first-page":"1180","DOI":"10.1039\/C9MH01824G","article-title":"Reconstructing the intracellular\npH microenvironment for enhancing\nphotodynamic therapy","volume":"7","author":"Wang","year":"2020","journal-title":"Mater. Horiz."},{"key":"2026083118453994600_cit8","doi-asserted-by":"publisher","first-page":"20677","DOI":"10.1021\/acsami.3c00844","article-title":"Surface-Roughened\nSERS-Active Single Silver Nanowire for Simultaneous Detection of Intracellular\nand Extracellular pHs","volume":"15","author":"Fang","year":"2023","journal-title":"ACS Appl. Mater. 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Chem."},{"key":"2026083118453994600_cit12","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05261-x","article-title":"Systems analysis\nof\nintracellular pH vulnerabilities for cancer therapy","volume":"9","author":"Persi","year":"2018","journal-title":"Nat. Commun."},{"key":"2026083118453994600_cit13","doi-asserted-by":"publisher","first-page":"7837","DOI":"10.1021\/acs.analchem.6b01932","article-title":"Label-Free Carbon-Dots-Based\nRatiometric Fluorescence pH Nanoprobes for Intracellular pH Sensing","volume":"88","author":"Shangguan","year":"2016","journal-title":"Anal. 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Actuators,\nB"},{"key":"2026083118453994600_cit18","doi-asserted-by":"publisher","first-page":"9282","DOI":"10.1002\/anie.202004326","article-title":"Monitoring\nH2O2 on the Surface of Single Cells with\nLiquid Crystal Elastomer Microspheres","volume":"59","author":"Li","year":"2020","journal-title":"Angew.\nChem., Int. Ed."},{"key":"2026083118453994600_cit19","doi-asserted-by":"publisher","first-page":"11472","DOI":"10.1021\/acs.analchem.4c02040","article-title":"Real-Time pH Sensor in Bacterial Microenvironments Using Liquid Crystal\nCore\u2013Shell Microspheres","volume":"96","author":"Xie","year":"2024","journal-title":"Anal. Chem."},{"key":"2026083118453994600_cit20","doi-asserted-by":"publisher","DOI":"10.1002\/admt.202501574","article-title":"A Liquid Crystal\nIntegrated Skin pH Sensing Patch Enabled\nby Optical Coherence Tomography","author":"Xu","year":"2025","journal-title":"Adv. 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B: Chem."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c02453","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/33\/24270\/66533951\/acs.analchem.6c02453.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/33\/24270\/66533951\/acs.analchem.6c02453.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T22:45:48Z","timestamp":1788216348000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/33\/24270\/5254714\/Internalized-Liquid-Crystal-Microcapsules-for-pH"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,13]]},"references-count":23,"journal-issue":{"issue":"33","published-online":{"date-parts":[[2026,8,13]]},"published-print":{"date-parts":[[2026,8,25]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c02453","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,13]]}}}