{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:41:09Z","timestamp":1792784469652,"version":"4.5.0"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"39","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100016692","name":"Key Research and Development Program of Ningxia","doi-asserted-by":"publisher","award":["2023BEG02014"],"award-info":[{"award-number":["2023BEG02014"]}],"id":[{"id":"10.13039\/100016692","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004772","name":"Natural Science Foundation of Ningxia Province","doi-asserted-by":"publisher","award":["2024AAC02043"],"award-info":[{"award-number":["2024AAC02043"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004179","name":"Ningxia Medical University","doi-asserted-by":"publisher","award":["XJKF240324"],"award-info":[{"award-number":["XJKF240324"]}],"id":[{"id":"10.13039\/501100004179","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004179","name":"Ningxia Medical University","doi-asserted-by":"publisher","award":["XJKF240330"],"award-info":[{"award-number":["XJKF240330"]}],"id":[{"id":"10.13039\/501100004179","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Natural Science Foundation of China (NSFC)","award":["82360684"],"award-info":[{"award-number":["82360684"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Exosomes, nanoscale extracellular vesicles carrying native membrane proteins, play crucial roles in intercellular communication and are critically involved in colorectal cancer progression and drug resistance, thereby offering a biologically relevant platform for targeted drug discovery. In this study, we developed an innovative immobilized exosome affinity screening platform (Exo@SiO2\u2013NH2) using HT-29 cell-derived exosomes to selectively capture antitumor bioactive compounds from Sophora flavescens. Through UHPLC-MS\/MS analysis, four compounds\u2500Kushenol I, Kurarinone, 2\u2032-O-MethylKurarinone, and Isoxanthohumol were successfully enriched and identified. To explore potential exosome-associated targets, systematic molecular docking and molecular dynamics simulations were conducted on representative exosome-enriched proteins against these four compounds. Further combinatorial alanine scanning mutagenesis within the predicted binding pockets (&amp;lt;5.0 \u00c5) suggested CD81 and HSP90 as probable protein targets. Subsequent functional validation via siRNA-mediated knockdown of CD81 and HSP90, combined with IC50 determination via CCK-8 assays, confirmed target-dependent alterations in drug responsiveness, supporting their role as key functional mediators. Together, this integrated strategy provides a practical and novel approach for discovering anti-colorectal cancer molecules targeting exosome-associated protein interfaces.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c02802","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:02:22Z","timestamp":1790355742000},"page":"28834-28846","source":"Crossref","is-referenced-by-count":0,"title":["An Exosome-Based\nAffinity Screening Platform for Identifying\nBioactive Compounds against Colon Cancer"],"prefix":"10.1021","volume":"98","author":[{"given":"Zhigang","family":"Yang","sequence":"first","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Yuan","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Guo","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Wang","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiman","family":"Zhang","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5231-8884","authenticated-orcid":true,"given":"Gidion","family":"Wilson","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingui","family":"Ma","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Yang","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Ma","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuping","family":"Sa","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyuan","family":"Li","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6258-8000","authenticated-orcid":true,"given":"Guoning","family":"Chen","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4818-5967","authenticated-orcid":true,"given":"Xueqin","family":"Ma","sequence":"additional","affiliation":[{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]},{"name":"Ningxia Medical University , , 1160 Shenli Street , ,","place":["Yinchuan, China, 750004"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"2026100608082169400_cit1","doi-asserted-by":"publisher","first-page":"233","DOI":"10.3322\/caac.21772","article-title":"Colorectal\ncancer statistics, 2023","volume":"73","author":"Siegel","year":"2023","journal-title":"Ca-Cancer J. Clin."},{"key":"2026100608082169400_cit2","doi-asserted-by":"publisher","first-page":"7470","DOI":"10.3390\/ijms25137470","article-title":"Innovative Strategies\nto Combat 5-Fluorouracil\nResistance in Colorectal Cancer: The Role of Phytochemicals and Extracellular\nVesicles","volume":"25","author":"Barathan","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"2026100608082169400_cit3","doi-asserted-by":"publisher","first-page":"6967","DOI":"10.3390\/ijms25136967","article-title":"Current and\nEmerging Treatment Paradigms in Colorectal Cancer: Integrating Hallmarks\nof Cancer","volume":"25","author":"Salva De Torres","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"2026100608082169400_cit4","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1038\/nri855","article-title":"Exosomes: composition, biogenesis\nand function","volume":"2","author":"The\u0301ry","year":"2002","journal-title":"Nat. Rev. Immunol."},{"key":"2026100608082169400_cit5","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau6977","article-title":"The biology, function,\nand biomedical applications\nof exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science"},{"key":"2026100608082169400_cit6","doi-asserted-by":"publisher","first-page":"575","DOI":"10.20892\/j.issn.2095-3941.2023.0119","article-title":"Regulatory role of exosomes in colorectal\ncancer progression and potential as biomarkers","volume":"20","author":"Hui","year":"2023","journal-title":"Cancer Biol. Med."},{"key":"2026100608082169400_cit7","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1080\/15384101.2024.2311501","article-title":"Small extracellular\nvesicles promote\nthe formation of the pre-metastatic niche through multiple mechanisms\nin colorectal cancer","volume":"23","author":"Wu","year":"2024","journal-title":"Cell Cycle"},{"key":"2026100608082169400_cit8","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1186\/s12951-023-01888-1","article-title":"The application\nof extracellular vesicles in colorectal cancer metastasis and drug\nresistance: recent advances and trends","volume":"21","author":"Xiong","year":"2023","journal-title":"J. Nanobiotechnol."},{"key":"2026100608082169400_cit9","doi-asserted-by":"publisher","first-page":"100163","DOI":"10.1016\/j.medidd.2023.100163","article-title":"Exploring potential of exosomes drug\ndelivery system\nin the treatment of cancer: Advances and prospective","volume":"20","author":"Yadav","year":"2023","journal-title":"Med. Drug Discovery"},{"key":"2026100608082169400_cit10","doi-asserted-by":"publisher","first-page":"107179","DOI":"10.1016\/j.phrs.2024.107179","article-title":"Treating cancer through modulating\nexosomal protein loading and function: The prospects of natural products\nand traditional Chinese medicine","volume":"203","author":"Meng","year":"2024","journal-title":"Pharmacol.\nRes."},{"key":"2026100608082169400_cit11","doi-asserted-by":"publisher","first-page":"844782","DOI":"10.3389\/fphar.2022.844782","article-title":"Exosomes: A novel insight\ninto traditional Chinese medicine","volume":"13","author":"Mo","year":"2022","journal-title":"Front. Pharmacol"},{"key":"2026100608082169400_cit12","doi-asserted-by":"publisher","first-page":"216261","DOI":"10.1016\/j.canlet.2023.216261","article-title":"Targeting tumor-derived\nexosome-mediated premetastatic niche formation: The metastasis-preventive\nvalue of traditional Chinese medicine","volume":"567","author":"Jia","year":"2023","journal-title":"Cancer\nLett."},{"key":"2026100608082169400_cit13","doi-asserted-by":"publisher","first-page":"1110922","DOI":"10.3389\/fphar.2023.1110922","article-title":"Role of exosomes\nin hepatocellular\ncarcinoma and the regulation of traditional Chinese medicine","volume":"14","author":"Yao","year":"2023","journal-title":"Front. Pharmacol"},{"key":"2026100608082169400_cit14","doi-asserted-by":"publisher","first-page":"122332","DOI":"10.1016\/j.jchromb.2020.122332","article-title":"Affinity chromatography: A review\nof trends and developments\nover the past 50 years","volume":"1157","author":"Rodriguez","year":"2020","journal-title":"J. Chromatogr. B: Anal.\nTechnol. Biomed. Life Sci."},{"key":"2026100608082169400_cit15","doi-asserted-by":"publisher","first-page":"114102","DOI":"10.1016\/j.jpba.2021.114102","article-title":"NOX2 inhibitor GSK2795039\nmetabolite identification towards drug optimization","volume":"201","author":"Padilha","year":"2021","journal-title":"J. Pharm. Biomed. Anal."},{"key":"2026100608082169400_cit16","doi-asserted-by":"publisher","first-page":"116419","DOI":"10.1016\/j.trac.2021.116419","article-title":"Immobilized-enzyme reactors integrated into analytical\nplatforms: Recent advances and challenges","volume":"144","author":"Wouters","year":"2021","journal-title":"TrAC,\nTrends Anal. Chem."},{"key":"2026100608082169400_cit17","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1080\/07388551.2021.2025034","article-title":"Immobilized\nenzyme for screening and identification of anti-diabetic components\nfrom natural products by ligand fishing","volume":"43","author":"Guo","year":"2023","journal-title":"Crit.\nRev. Biotechnol."},{"key":"2026100608082169400_cit18","doi-asserted-by":"publisher","first-page":"116510","DOI":"10.1016\/j.bios.2024.116510","article-title":"A strategy for inhibitors\nscreening of xanthine oxidase\nbased on colorimetric sensor combined with affinity chromatography\ntechnology","volume":"261","author":"Chen","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"2026100608082169400_cit19","doi-asserted-by":"publisher","first-page":"111044","DOI":"10.1016\/j.biopha.2020.111044","article-title":"Anticancer\nactivities of TCM and their active components against tumor metastasis","volume":"133","author":"Wang","year":"2021","journal-title":"Biomed Pharmacother."},{"key":"2026100608082169400_cit20","doi-asserted-by":"publisher","first-page":"4507","DOI":"10.21037\/tcr-2025-359","article-title":"Exploration of the\nmechanism of curcumin in the regulation of apoptosis for the treatment\nof colorectal cancer","volume":"14","author":"Wu","year":"2025","journal-title":"Transl. Cancer Res."},{"key":"2026100608082169400_cit21","doi-asserted-by":"publisher","first-page":"956793","DOI":"10.3389\/fonc.2022.956793","article-title":"Inhibition\nand potential treatment of colorectal cancer by natural\ncompounds via various signaling pathways","volume":"12","author":"Wang","year":"2022","journal-title":"Front.\nOncol"},{"key":"2026100608082169400_cit22","doi-asserted-by":"publisher","first-page":"2787","DOI":"10.2147\/DDDT.S417077","article-title":"Pharmacological\nActivity of Matrine in Inhibiting Colon Cancer Cells VM Formation,\nProliferation, and Invasion by Downregulating Claudin-9 Mediated EMT\nProcess and MAPK Signaling Pathway","volume":"17","author":"Du","year":"2023","journal-title":"Drug Des.,\nDev. Ther."},{"key":"2026100608082169400_cit23","doi-asserted-by":"publisher","first-page":"105778","DOI":"10.1016\/j.phrs.2021.105778","article-title":"Biological\neffects and mechanisms of matrine and other constituents of Sophora\nflavescens in colorectal cancer","volume":"171","author":"Chen","year":"2021","journal-title":"Pharmacol.\nRes."},{"key":"2026100608082169400_cit24","doi-asserted-by":"publisher","first-page":"842295","DOI":"10.3389\/fphar.2022.842295","article-title":"Traditional\nChinese Medicines as Effective Reversals\nof Epithelial-Mesenchymal Transition Induced-Metastasis of Colorectal\nCancer: Molecular Targets and Mechanisms","volume":"13","author":"Ge","year":"2022","journal-title":"Front.\nPharmacol"},{"key":"2026100608082169400_cit25","doi-asserted-by":"publisher","first-page":"123456","DOI":"10.1016\/j.ijpharm.2023.123456","article-title":"Colorectal\ncancer cell exosome and cytoplasmic membrane for homotypic delivery\nof therapeutic molecules","volume":"646","author":"Jarak","year":"2023","journal-title":"Int. J Pharm."},{"key":"2026100608082169400_cit26","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.3390\/ijms25021153","article-title":"Establishment of Polydopamine-Modified HK-2 Cell Membrane\nChromatography\nand Screening of Active Components from Plantago asiatica L","volume":"25","author":"Gao","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"2026100608082169400_cit27","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.1016\/S1875-5364(25)60982-4","article-title":"Research progress on\nnew techniques and methods for identifying active ingredients in traditional\nChinese medicine","volume":"23","author":"Zhang","year":"2025","journal-title":"Chin. J. Nat. Med."},{"key":"2026100608082169400_cit28","doi-asserted-by":"publisher","first-page":"113829","DOI":"10.1016\/j.jconrel.2025.113829","article-title":"Cell membrane derived\nbiomimetic nanomedicine for precision delivery of traditional Chinese\nmedicine in cancer therapy","volume":"383","author":"Xiao","year":"2025","journal-title":"J. Controlled Release"},{"key":"2026100608082169400_cit29","doi-asserted-by":"publisher","first-page":"D1670","DOI":"10.1093\/nar\/gkae973","article-title":"Generic\nDiagramming Platform (GDP): a comprehensive database of high-quality\nbiomedical graphics","volume":"53","author":"Jiang","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"2026100608082169400_cit30","doi-asserted-by":"publisher","first-page":"1032","DOI":"10.1038\/s41598-017-01198-1","article-title":"Affinity biosensors using recombinant\nnative membrane\nproteins displayed on exosomes: application to botulinum neurotoxin\nB receptor","volume":"7","author":"Desplantes","year":"2017","journal-title":"Sci. Rep"},{"key":"2026100608082169400_cit31","doi-asserted-by":"publisher","first-page":"iyae88","DOI":"10.1093\/genetics\/iyae088","article-title":"Extracellular vesicles","volume":"227","author":"Wang","year":"2024","journal-title":"Genetics"},{"key":"2026100608082169400_cit32","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1016\/j.cell.2020.07.009","article-title":"Extracellular Vesicle\nand Particle Biomarkers Define Multiple Human Cancers","volume":"182","author":"Hoshino","year":"2020","journal-title":"Cell"},{"key":"2026100608082169400_cit33","doi-asserted-by":"publisher","first-page":"1535750","DOI":"10.1080\/20013078.2018.1461450","article-title":"Minimal information for studies of extracellular\nvesicles 2018 (MISEV2018): a position statement of the International\nSociety for Extracellular Vesicles and update of the MISEV2014 guidelines","volume":"7","author":"The\u0301ry","year":"2018","journal-title":"J. Extracell. Vesicles"},{"key":"2026100608082169400_cit34","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1038\/s41556-021-00693-y","article-title":"Quantitative\nproteomics identifies the core proteome of exosomes with syntenin-1\nas the highest abundant protein and a putative universal biomarker","volume":"23","author":"Kugeratski","year":"2021","journal-title":"Nat. Cell Biol."},{"key":"2026100608082169400_cit35","doi-asserted-by":"publisher","first-page":"2100","DOI":"10.1038\/nprot.2014.138","article-title":"The cellular thermal\nshift assay for evaluating drug target interactions in cells","volume":"9","author":"Jafari","year":"2014","journal-title":"Nat. Protoc."},{"key":"2026100608082169400_cit36","doi-asserted-by":"publisher","first-page":"6211","DOI":"10.1021\/acs.analchem.4c07092","article-title":"Precise Identification of Inhibitors for Coagulation\nReactions from\nComplex Extracts through Monitoring of Biological Aggregates Combined\nwith a Targeted Fishing Technique","volume":"97","author":"Zhang","year":"2025","journal-title":"Anal. Chem."},{"key":"2026100608082169400_cit37","doi-asserted-by":"publisher","first-page":"101506","DOI":"10.1016\/j.jscs.2022.101506","article-title":"One-step continuous\nflow synthesis\nof aminopropyl silica-coated magnetite nanoparticles","volume":"26","author":"Mokkarat","year":"2022","journal-title":"J. Saudi Chem. Soc."},{"key":"2026100608082169400_cit38","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12404","article-title":"Minimal information\nfor studies of extracellular vesicles (MISEV2023): From basic to advanced\napproaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"2026100608082169400_cit39","doi-asserted-by":"publisher","first-page":"9915027","DOI":"10.1155\/2021\/9915027","article-title":"Determination of Alkaloids and Flavonoids in Sophora\nflavescens by UHPLC-Q-TOF\/MS","volume":"2021","author":"Dong","year":"2021","journal-title":"J. Anal. Methods\nChem."},{"key":"2026100608082169400_cit40","doi-asserted-by":"publisher","first-page":"1019","DOI":"10.1016\/j.jpba.2007.04.019","article-title":"Characterization of flavonoids in\nthe extract of Sophora flavescens Ait. by high-performance liquid\nchromatography coupled with diode-array detector and electrospray\nionization mass spectrometry","volume":"44","author":"Zhang","year":"2007","journal-title":"J. Pharm. Biomed\nAnal."},{"key":"2026100608082169400_cit41","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1016\/j.chroma.2006.08.019","article-title":"Analysis of xanthohumol\nand isoxanthohumol in different hop products by liquid chromatography-diode\narray detection-electrospray ionization tandem mass spectrometry","volume":"1150","author":"Magalha\u0303es","year":"2007","journal-title":"J. Chromatogr A."},{"key":"2026100608082169400_cit42","doi-asserted-by":"publisher","first-page":"2548","DOI":"10.3390\/ijms26062548","article-title":"Evaluation\nof the Inhibitory Potential of Apigenin and Related Flavonoids on\nVarious Proteins Associated with Human Diseases Using AutoDock","volume":"26","author":"Peanlikhit","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"2026100608082169400_cit43","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1038\/nrc1716","article-title":"HSP90 and the chaperoning of cancer","volume":"5","author":"Whitesell","year":"2005","journal-title":"Nat. Rev. Cancer"},{"key":"2026100608082169400_cit44","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1038\/nrc2887","article-title":"Targeting the dynamic\nHSP90 complex in cancer","volume":"10","author":"Trepel","year":"2010","journal-title":"Nat. Rev. Cancer."},{"key":"2026100608082169400_cit45","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1158\/1078-0432.CCR-11-1000","article-title":"Hsp90 Molecular Chaperone\nInhibitors: Are We There\nYet?","volume":"18","author":"Neckers","year":"2012","journal-title":"Nat. Rev. Cancer"}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c02802","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/39\/28834\/68661153\/acs.analchem.6c02802.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/39\/28834\/68661153\/acs.analchem.6c02802.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:49:39Z","timestamp":1791290979000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/39\/28834\/5439007\/An-Exosome-Based-Affinity-Screening-Platform-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,25]]},"references-count":45,"journal-issue":{"issue":"39","published-online":{"date-parts":[[2026,9,25]]},"published-print":{"date-parts":[[2026,10,6]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c02802","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,25]]}}}