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While electrochemiluminescence (ECL) has emerged as a powerful analytical technique owing to its low background interference and high sensitivity, the performance of MOF-based ECL systems is often limited by sluggish interfacial charge transfer and insufficient co-reactant activation at a single type of catalytic site. To overcome these bottlenecks, a two-dimensional (2D) ZnCu metal-organic framework\/TiO2 (ZCMT) heterojunction sensing system was constructed by coupling interface engineering with a dual-site synergistic catalysis strategy. Specifically, the heterojunction facilitates directional charge transport by promoting electron delocalization and interfacial charge redistribution. Concurrently, the Cu- and Ti-based redox-active centers synergistically facilitate K2S2O8 activation and accelerate its reduction. This synergistic mechanism substantially enhances its ECL performance. By integrating the ZCMT platform with DNAzyme-assisted amplification strategy, a high-performance ECL biosensor was constructed for the ultrasensitive quantification of \u03b1-Syn. Overall, this study provides an effective strategy for overcoming the charge-transfer and catalytic limitations of MOF-based ECL systems and establishes a promising platform for the ultrasensitive analysis of \u03b1-Syn, with potential applications in the early screeningof Parkinson\u2019s disease.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c02862","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T18:25:52Z","timestamp":1784658352000},"page":"22409-22417","source":"Crossref","is-referenced-by-count":0,"title":["Heterojunction-Mediated\nDual-Site Synergistic Catalysis\nfor Enhanced Electrochemiluminescence Detection of \u03b1-Synuclein"],"prefix":"10.1021","volume":"98","author":[{"given":"Yamei","family":"Li","sequence":"first","affiliation":[{"name":"University of Jinan , , ,","place":["Jinan, P. R. 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