{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T02:36:08Z","timestamp":1792550168872,"version":"4.5.0"},"reference-count":55,"publisher":"American Chemical Society (ACS)","issue":"38","license":[{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG088147"],"award-info":[{"award-number":["R01AG088147"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM124755"],"award-info":[{"award-number":["R35GM124755"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Distrito Federal Research Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Low-input and single-cell proteomics by mass spectrometry (MS) have advanced rapidly, but balancing analytical speed and sensitivity remains challenging, especially for small, protein-scarce primary cells such as neutrophils. Here, we report Rapid Eco\u2013AI, an optimized electrophoresis-correlative (Eco) capillary electrophoresis\u2013mass spectrometry (CE\u2013MS) workflow with artificial intelligence (AI)-assisted data processing for fast, high-efficiency, low-input proteome profiling. Relative to our 15 min Eco\u2013AI method, Rapid Eco\u2013AI compresses the effective separation window to 5\u20137 min while maintaining substantial peptide and proteome coverage, efficient precursor ion sampling, and quantitative precision. Real-time tracking of charge-dependent m\/z and migration-time correlations, together with an optimized MS2 strategy, sustained high peptide-spectrum-match conversion under rapid CE, while AI-assisted interpretation of chimeric MS2 spectra (CHIMERYS) supported robust identification and label-free quantification. Implemented on a legacy Orbitrap platform (Q Exactive Plus), Rapid Eco\u2013AI cumulatively identified 1,634 and 1,347 proteins from 300 pg of single-cell-equivalent HeLa digest across technical triplicates using 7 and 5 min effective separations, corresponding to &amp;lt;15 and &amp;lt;11 min injection-to-injection runtimes, respectively. Using the 7 min separation, 835 proteins were cumulatively identified from 75 pg of HeLa digest. Hybrid HeLa\u2013yeast proteome mixtures validated quantitative performance, with median coefficients of variation &amp;lt;5% and measured mean B\/A ratios of 0.57 and 1.60 for expected values of 0.60 and 1.80, respectively. As a proof-of-principle application, we analyzed fractional digest loads from 13 freshly isolated single human neutrophils, corresponding to \u223c2 pg of digest per run on a manual CE\u2013MS platform, and identified 135 proteins after conservative background filtering. Collectively, these results establish Rapid Eco\u2013AI as a fast CE\u2013MS workflow for low-input proteome profiling with quantitative validation and proof-of-principle transfer to neutrophil-derived digest analysis.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03046","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T19:01:47Z","timestamp":1789239707000},"page":"28097-28105","source":"Crossref","is-referenced-by-count":1,"title":["Rapid Eco-AI\nCE\u2013MS Enables Sub-7 Min\nLow-Input Proteome Profiling with Quantitative Validation"],"prefix":"10.1021","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4461-2423","authenticated-orcid":true,"given":"Fei","family":"Zhou","sequence":"first","affiliation":[{"name":"University of Maryland , , , ,","place":["College Park, Maryland, United States, 20742"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowen","family":"Shen","sequence":"additional","affiliation":[{"name":"University of Maryland , , , ,","place":["College Park, Maryland, United States, 20742"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8722-5103","authenticated-orcid":true,"given":"Isabelle S.","family":"Luz","sequence":"additional","affiliation":[{"name":"University of Maryland , , , ,","place":["College Park, Maryland, United States, 20742"]},{"name":"University of Brasi\u0301lia , , , DF ,","place":["Brasi\u0301lia, Brazil, 70910-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3610-471X","authenticated-orcid":true,"given":"Laura G.","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"University of Maryland , , , ,","place":["College Park, Maryland, United States, 20742"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4627-6489","authenticated-orcid":true,"given":"Steven J.","family":"Prior","sequence":"additional","affiliation":[{"name":"University of Maryland , , , ,","place":["College Park, Maryland, United States, 20742"]},{"name":"Education & Clinical Center , , , ,","place":["Baltimore, Maryland, United States, 21201"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5140-8573","authenticated-orcid":true,"given":"Wagner","family":"Fontes","sequence":"additional","affiliation":[{"name":"University of Brasi\u0301lia , , , DF ,","place":["Brasi\u0301lia, Brazil, 70910-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4704-4997","authenticated-orcid":true,"given":"Peter","family":"Nemes","sequence":"additional","affiliation":[{"name":"University of Maryland , , , ,","place":["College Park, Maryland, United States, 20742"]},{"name":"University of Maryland , , , ,","place":["College Park, Maryland, United States, 20742"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,12]]},"reference":[{"issue":"3","key":"2026092909094967900_cit1","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1038\/s41592-023-01791-5","article-title":"Single-cell proteomics\nenabled by next-generation sequencing or mass spectrometry","volume":"20","author":"Bennett","year":"2023","journal-title":"Nat. Methods"},{"key":"2026092909094967900_cit2","doi-asserted-by":"publisher","first-page":"117117","DOI":"10.1016\/j.trac.2023.117117","article-title":"Single-cell\nomic\nmolecular profiling using capillary electrophoresis mass spectrometry","volume":"165","author":"Bagwe","year":"2023","journal-title":"Trends Anal. Chem."},{"issue":"18","key":"2026092909094967900_cit3","doi-asserted-by":"publisher","first-page":"2173","DOI":"10.1182\/blood-2016-01-688887","article-title":"Neutrophil\nheterogeneity: implications\nfor homeostasis and pathogenesis","volume":"127","author":"Silvestre-Roig","year":"2016","journal-title":"Blood"},{"issue":"3","key":"2026092909094967900_cit4","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1038\/nri3399","article-title":"Neutrophil recruitment\nand function in health and inflammation","volume":"13","author":"Kolaczkowska","year":"2013","journal-title":"Nat.\nRev. Immunol."},{"issue":"9","key":"2026092909094967900_cit5","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.1038\/s41590-020-0736-z","article-title":"Single-cell transcriptome profiling reveals neutrophil\nheterogeneity in homeostasis and infection","volume":"21","author":"Xie","year":"2020","journal-title":"Nat. Immunol."},{"issue":"1","key":"2026092909094967900_cit6","doi-asserted-by":"publisher","first-page":"5966","DOI":"10.1038\/s41598-024-56163-6","article-title":"Quantitative\nproteomics reveals tissue-specific, infection-induced and species-specific\nneutrophil protein signatures","volume":"14","author":"Sollberger","year":"2024","journal-title":"Sci. Rep."},{"issue":"1","key":"2026092909094967900_cit7","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1186\/1477-5956-7-32","article-title":"Proteomic analysis\nof total cellular proteins of human neutrophils","volume":"7","author":"Tomazella","year":"2009","journal-title":"Proteome Sci."},{"issue":"11","key":"2026092909094967900_cit8","doi-asserted-by":"publisher","first-page":"100858","DOI":"10.1016\/j.mcpro.2024.100858","article-title":"Proteomic\ncharacterization of 1000 human and murine neutrophils freshly isolated\nfrom blood and sites of sterile inflammation","volume":"23","author":"Ghosh","year":"2024","journal-title":"Mol. Cell. Proteomics"},{"issue":"1","key":"2026092909094967900_cit9","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1038\/s41467-025-67367-3","article-title":"Single cell proteomic analysis defines\ndiscrete neutrophil functional states in human glioblastoma","volume":"17","author":"Sadiku","year":"2026","journal-title":"Nat. Commun."},{"key":"2026092909094967900_cit10","doi-asserted-by":"publisher","first-page":"106\u2013138","DOI":"10.1002\/mas.21824","article-title":"Biological\nmass spectrometry enables spatiotemporal \u2019omics: From tissues\nto cells to organelles","volume":"43","author":"Pade","year":"2024","journal-title":"Mass Spectrom. Rev."},{"issue":"1","key":"2026092909094967900_cit11","doi-asserted-by":"publisher","first-page":"42","DOI":"10.3390\/molecules24010042","article-title":"Recent advances\nand new perspectives in capillary electrophoresis mass spectrometry\nfor single cell omics","volume":"24","author":"DeLaney","year":"2018","journal-title":"Molecules"},{"issue":"4","key":"2026092909094967900_cit12","doi-asserted-by":"publisher","first-page":"1519","DOI":"10.1021\/acs.jproteome.4c00277","article-title":"Dynamics of single-cell\nprotein covariation during epithelial-mesenchymal transition","volume":"24","author":"Khan","year":"2025","journal-title":"J. Proteome Res."},{"issue":"1","key":"2026092909094967900_cit13","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1186\/s13059-021-02267-5","article-title":"Single-cell\nproteomic and transcriptomic analysis of macrophage heterogeneity\nusing SCoPE2","volume":"22","author":"Specht","year":"2021","journal-title":"Genome Biol."},{"issue":"8","key":"2026092909094967900_cit14","doi-asserted-by":"publisher","first-page":"1701","DOI":"10.1021\/jasms.3c00159","article-title":"Rapid,\none-step sample processing for label-free single-cell proteomics","volume":"34","author":"Johnston","year":"2023","journal-title":"J. Am. Soc. Mass Spectrom."},{"issue":"1","key":"2026092909094967900_cit15","doi-asserted-by":"publisher","first-page":"6246","DOI":"10.1038\/s41467-021-26514-2","article-title":"High-throughput and high-efficiency sample preparation for single-cell\nproteomics using a nested nanowell chip","volume":"12","author":"Woo","year":"2021","journal-title":"Nat.\nCommun."},{"issue":"3","key":"2026092909094967900_cit16","doi-asserted-by":"publisher","first-page":"e10798","DOI":"10.15252\/msb.202110798","article-title":"Ultra-high\nsensitivity mass spectrometry quantifies single-cell proteome changes\nupon perturbation","volume":"18","author":"Brunner","year":"2022","journal-title":"Mol. Syst. Biol."},{"issue":"12","key":"2026092909094967900_cit17","doi-asserted-by":"publisher","first-page":"5398","DOI":"10.1038\/s41596-021-00616-z","article-title":"Multiplexed single-cell\nproteomics\nusing SCoPE2","volume":"16","author":"Petelski","year":"2021","journal-title":"Nat. Protoc."},{"issue":"15","key":"2026092909094967900_cit18","doi-asserted-by":"publisher","first-page":"6017","DOI":"10.1021\/acs.analchem.2c00646","article-title":"Label-free\nprofiling of up to 200 single-cell proteomes per day using a dual-column\nnanoflow liquid chromatography platform","volume":"94","author":"Webber","year":"2022","journal-title":"Anal.\nChem."},{"issue":"4","key":"2026092909094967900_cit19","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1074\/mcp.TIR119.001906","article-title":"A compact\nquadrupole-orbitrap mass spectrometer with FAIMS interface improves\nproteome coverage in short LC gradients","volume":"19","author":"Bekker-Jensen","year":"2020","journal-title":"Mol.\nCell. Proteomics"},{"issue":"24","key":"2026092909094967900_cit20","doi-asserted-by":"publisher","first-page":"9145","DOI":"10.1021\/acs.analchem.3c00213","article-title":"High-throughput\nsingle-cell proteomic analysis of organ-derived heterogeneous cell\npopulations by nanoflow dual-trap single-column liquid chromatography","volume":"95","author":"Kreimer","year":"2023","journal-title":"Anal. Chem."},{"issue":"12","key":"2026092909094967900_cit21","doi-asserted-by":"publisher","first-page":"100665","DOI":"10.1016\/j.mcpro.2023.100665","article-title":"An automated\nnanowell-array\nworkflow for quantitative multiplexed single-cell proteomics sample\npreparation at high sensitivity","volume":"22","author":"Ctortecka","year":"2023","journal-title":"Mol. Cell.\nProteomics"},{"issue":"3","key":"2026092909094967900_cit22","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1038\/s41592-024-02558-2","article-title":"Enhanced sensitivity\nand scalability with a Chip-Tip workflow enables deep single-cell\nproteomics","volume":"22","author":"Ye","year":"2025","journal-title":"Nat. Methods"},{"key":"2026092909094967900_cit23","doi-asserted-by":"publisher","first-page":"1549","DOI":"10.1021\/acs.jproteome.4c00491","article-title":"Data-independent\nacquisition shortens\nthe analytical window of single-cell proteomics to fifteen minutes\nin capillary electrophoresis mass spectrometry","volume":"24","author":"Shen","year":"2025","journal-title":"J. Proteome Res."},{"issue":"51","key":"2026092909094967900_cit24","doi-asserted-by":"publisher","first-page":"13661","DOI":"10.1002\/anie.201308139","article-title":"Ultrasensitive\nand fast bottom-up analysis of femtogram amounts of complex proteome\ndigests","volume":"52","author":"Sun","year":"2013","journal-title":"Angew. Chem., Int. Ed."},{"issue":"3","key":"2026092909094967900_cit25","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1002\/rcm.6786","article-title":"Ultrafast capillary\nelectrophoresis\/mass spectrometry\nwith adjustable porous tip for a rapid analysis of protein digest\nin about a minute","volume":"28","author":"Moini","year":"2014","journal-title":"Rapid Commun. Mass Spectrom."},{"issue":"45","key":"2026092909094967900_cit26","doi-asserted-by":"publisher","first-page":"e202510692","DOI":"10.1002\/anie.202510692","article-title":"Real-time\nEco\u2013AI, electrophoresis-correlative\ndata-dependent acquisition with AI-based data processing broadens\naccess to single-cell mass spectrometry proteomics","volume":"64","author":"Shen","year":"2025","journal-title":"Angew. Chem., Int. Ed."},{"issue":"20","key":"2026092909094967900_cit27","doi-asserted-by":"publisher","first-page":"8505","DOI":"10.1021\/ac301091m","article-title":"Improving the comprehensiveness and sensitivity of\nsheathless capillary electrophoresis tandem mass spectrometry for\nproteomic analysis","volume":"84","author":"Wang","year":"2012","journal-title":"Anal. Chem."},{"issue":"13","key":"2026092909094967900_cit28","doi-asserted-by":"publisher","first-page":"6331","DOI":"10.1021\/ac5004486","article-title":"Bottom-up proteomics\nof Escherichia coli using dynamic pH junction preconcentration\nand capillary zone electrophoresis electrospray ionization tandem\nmass spectrometry","volume":"86","author":"Zhu","year":"2014","journal-title":"Anal. Chem."},{"issue":"8","key":"2026092909094967900_cit29","doi-asserted-by":"publisher","first-page":"4572","DOI":"10.1021\/acs.analchem.5b00789","article-title":"Detachable\nstrong cation exchange monolith, integrated with capillary zone electrophoresis\nand coupled with pH gradient elution, produces improved sensitivity\nand numbers of peptide identifications during bottom-up analysis of\ncomplex proteomes","volume":"87","author":"Zhang","year":"2015","journal-title":"Anal. Chem."},{"issue":"12","key":"2026092909094967900_cit30","doi-asserted-by":"publisher","first-page":"2118","DOI":"10.1039\/C7AN00509A","article-title":"Capillary\nzone electrophoresis-mass\nspectrometry with microliter-scale loading capacity, 140 min separation\nwindow and high peak capacity for bottom-up proteomics","volume":"142","author":"Chen","year":"2017","journal-title":"Analyst"},{"issue":"25","key":"2026092909094967900_cit31","doi-asserted-by":"publisher","first-page":"9018","DOI":"10.1021\/acs.analchem.2c01062","article-title":"Microanalysis of brain angiotensin\npeptides using ultrasensitive capillary electrophoresis trapped ion\nmobility mass spectrometry","volume":"94","author":"DeLaney","year":"2022","journal-title":"Anal. Chem."},{"key":"2026092909094967900_cit32","doi-asserted-by":"publisher","first-page":"17841","DOI":"10.1021\/acs.analchem.5c03550","article-title":"Online coupling of\nacoustic droplet levitation with capillary electrophoresis\nmass spectrometry enables midair enrichment for improved proteomic\nsensitivity","volume":"97","author":"Jia","year":"2025","journal-title":"Anal. Chem."},{"issue":"8","key":"2026092909094967900_cit33","doi-asserted-by":"publisher","first-page":"2756","DOI":"10.1074\/mcp.M115.057760","article-title":"Label-free\nquantification\nof proteins in single embryonic cells with neural fate in the cleavage-stage\nfrog (Xenopus laevis) embryo using capillary electrophoresis\nelectrospray ionization high-resolution mass spectrometry (CE-ESI-HRMS)","volume":"15","author":"Lombard-Banek","year":"2016","journal-title":"Mol. Cell. Proteomics"},{"issue":"7","key":"2026092909094967900_cit34","doi-asserted-by":"publisher","first-page":"4797","DOI":"10.1021\/acs.analchem.9b00345","article-title":"Microsampling capillary\nelectrophoresis mass spectrometry enables single-cell proteomics in\ncomplex tissues: developing cell clones in live Xenopus laevis and zebrafish embryos","volume":"91","author":"Lombard-Banek","year":"2019","journal-title":"Anal. Chem."},{"issue":"5","key":"2026092909094967900_cit35","doi-asserted-by":"publisher","first-page":"2312","DOI":"10.1021\/acs.jproteome.5b00100","article-title":"Third-generation\nelectrokinetically pumped sheath-flow nanospray interface with improved\nstability and sensitivity for automated capillary zone electrophoresis\nmass spectrometry analysis of complex proteome digests","volume":"14","author":"Sun","year":"2015","journal-title":"J. Proteome Res."},{"issue":"23","key":"2026092909094967900_cit36","doi-asserted-by":"publisher","first-page":"12852","DOI":"10.1002\/anie.202100923","article-title":"In vivo subcellular\nmass spectrometry enables proteo-metabolomic single-cell systems biology\nin a chordate embryo developing to a normally behaving tadpole (X. laevis)","volume":"60","author":"Lombard-Banek","year":"2021","journal-title":"Angew. Chem., Int. Ed."},{"issue":"19","key":"2026092909094967900_cit37","doi-asserted-by":"publisher","first-page":"14516","DOI":"10.1021\/acs.analchem.6c01323","article-title":"Electrospray regime controls sensitivity\nand quantitative performance in micro- and nano-flow CE\u2013ESI\u2013MS","volume":"98","author":"O\u2019Neal","year":"2026","journal-title":"Anal. Chem."},{"issue":"9","key":"2026092909094967900_cit38","doi-asserted-by":"publisher","first-page":"2513","DOI":"10.1074\/mcp.M113.031591","article-title":"Accurate proteome-wide label-free quantification by delayed normalization\nand maximal peptide ratio extraction, termed MaxLFQ","volume":"13","author":"Cox","year":"2014","journal-title":"Mol. Cell. Proteomics"},{"issue":"20","key":"2026092909094967900_cit39","doi-asserted-by":"publisher","first-page":"14103","DOI":"10.1021\/acs.analchem.0c03129","article-title":"Capillary electrophoresis mass spectrometry\nat trial by metabo-ring: effective electrophoretic mobility for reproducible\nand robust compound annotation","volume":"92","author":"Drouin","year":"2020","journal-title":"Anal. Chem."},{"issue":"3","key":"2026092909094967900_cit40","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1038\/s41592-023-01785-3","article-title":"Initial recommendations\nfor performing, benchmarking and reporting single-cell proteomics\nexperiments","volume":"20","author":"Gatto","year":"2023","journal-title":"Nat. Methods"},{"issue":"1","key":"2026092909094967900_cit41","doi-asserted-by":"publisher","first-page":"882","DOI":"10.1038\/s41467-018-03367-w","article-title":"Nanodroplet\nprocessing platform for deep and quantitative proteome profiling of\n10-100 mammalian cells","volume":"9","author":"Zhu","year":"2018","journal-title":"Nat. Commun."},{"issue":"39","key":"2026092909094967900_cit42","doi-asserted-by":"publisher","first-page":"15581","DOI":"10.1021\/acs.analchem.4c02330","article-title":"Electrophoresis-correlative\ndata-independent\nacquisition (Eco-DIA) improves the sensitivity of mass spectrometry\nfor limited proteome amounts","volume":"96","author":"Shen","year":"2024","journal-title":"Anal. Chem."},{"issue":"D1","key":"2026092909094967900_cit43","doi-asserted-by":"publisher","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING\ndatabase in 2023: protein-protein association networks and functional\nenrichment analyses for any sequenced genome of interest","volume":"51","author":"Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res."},{"issue":"4","key":"2026092909094967900_cit44","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1002\/ijc.25991","article-title":"Tumor-derived\nmacrophage migration inhibitory factor modulates the biology of head\nand neck cancer cells via neutrophil activation","volume":"129","author":"Dumitru","year":"2011","journal-title":"Int. J. Cancer"},{"issue":"2","key":"2026092909094967900_cit45","doi-asserted-by":"publisher","first-page":"236","DOI":"10.3390\/cells11020236","article-title":"S100A8\/A9 is a marker for the release of neutrophil\nextracellular traps and induces neutrophil activation","volume":"11","author":"Sprenkeler","year":"2022","journal-title":"Cells"},{"issue":"1","key":"2026092909094967900_cit46","doi-asserted-by":"publisher","first-page":"176","DOI":"10.3390\/cancers15010176","article-title":"Single-cell transcriptomic\nprofiles of lung pre-metastatic niche reveal neutrophil and lymphatic\nendothelial cell roles in breast cancer","volume":"15","author":"Huang","year":"2022","journal-title":"Cancers"},{"issue":"2","key":"2026092909094967900_cit47","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1016\/j.bbamcr.2011.11.014","article-title":"Peroxiredoxin\n6\ntranslocates to the plasma membrane during neutrophil activation and\nis required for optimal NADPH oxidase activity","volume":"1823","author":"Ambruso","year":"2012","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"2026092909094967900_cit48","doi-asserted-by":"publisher","first-page":"16985","DOI":"10.1021\/acs.analchem.4c04353","article-title":"Development and validation of RoboCap, a robotic capillary\nplatform\nto automate capillary electrophoresis mass spectrometry en route to\nhigh-throughput single-cell proteomics","volume":"96","author":"Jia","year":"2024","journal-title":"Anal.\nChem."},{"issue":"1","key":"2026092909094967900_cit49","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1186\/s13059-018-1547-5","article-title":"SCoPE-MS: mass spectrometry\nof single mammalian cells quantifies proteome heterogeneity during\ncell differentiation","volume":"19","author":"Budnik","year":"2018","journal-title":"Genome Biol."},{"issue":"1","key":"2026092909094967900_cit50","doi-asserted-by":"publisher","first-page":"5707","DOI":"10.1038\/s41467-024-49651-w","article-title":"Automated single-cell\nproteomics\nproviding sufficient proteome depth to study complex biology beyond\ncell type classifications","volume":"15","author":"Ctortecka","year":"2024","journal-title":"Nat. Commun."},{"issue":"3","key":"2026092909094967900_cit51","doi-asserted-by":"publisher","first-page":"510","DOI":"10.1038\/s41592-024-02559-1","article-title":"Challenging\nthe Astral mass analyzer\nto quantify up to 5,300 proteins per single cell at unseen accuracy\nto uncover cellular heterogeneity","volume":"22","author":"Bubis","year":"2025","journal-title":"Nat. Methods"},{"issue":"5","key":"2026092909094967900_cit52","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1016\/j.cels.2022.02.003","article-title":"Three-dimensional feature\nmatching improves coverage for single-cell proteomics based on ion\nmobility filtering","volume":"13","author":"Woo","year":"2022","journal-title":"Cell Syst."},{"key":"2026092909094967900_cit53","doi-asserted-by":"publisher","first-page":"2453","DOI":"10.1021\/acs.jproteome.2c00337","article-title":"Coupling\nhigh-field asymmetric ion\nmobility spectrometry with capillary electrophoresis-electrospray\nionization-tandem mass spectrometry improves protein identifications\nin bottom-up proteomic analysis of low nanogram samples","volume":"21","author":"Johnson","year":"2022","journal-title":"J. Proteome Res."},{"issue":"2","key":"2026092909094967900_cit54","doi-asserted-by":"publisher","first-page":"100892","DOI":"10.1016\/j.mcpro.2024.100892","article-title":"Electrophoresis-correlative ion mobility\ndeepens single-cell proteomics in capillary electrophoresis mass spectrometry","volume":"24","author":"Shen","year":"2025","journal-title":"Mol. Cell. Proteomics"},{"issue":"51","key":"2026092909094967900_cit55","doi-asserted-by":"publisher","first-page":"17003","DOI":"10.1021\/acs.analchem.1c03477","article-title":"Sample size-comparable spectral library\nenhances data-independent acquisition-based proteome coverage of low-input\ncells","volume":"93","author":"Siyal","year":"2021","journal-title":"Anal. Chem."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c03046","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/38\/28097\/68492843\/acs.analchem.6c03046.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/38\/28097\/68492843\/acs.analchem.6c03046.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:35:46Z","timestamp":1790688946000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/38\/28097\/5428959\/Rapid-Eco-AI-CE-MS-Enables-Sub-7-Min-Low-Input"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,12]]},"references-count":55,"journal-issue":{"issue":"38","published-online":{"date-parts":[[2026,9,12]]},"published-print":{"date-parts":[[2026,9,29]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c03046","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,12]]}}}