{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T11:54:18Z","timestamp":1792065258562,"version":"4.4.0"},"reference-count":34,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32271514"],"award-info":[{"award-number":["32271514"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82302277"],"award-info":[{"award-number":["82302277"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Enthalpy-driven catalytic hairpin assembly (CHA) suffers from an inherent trade-off, wherein enhanced reaction kinetics is inevitably accompanied by elevated nonspecific background. Catalytic hairpin assembly-and-cyclization (CHAC) integrates CHA with enzymatic ligation, allowing single-target-triggered multiple circularization events and thus bypassing the stoichiometric limitation of conventional padlock probe ligation. Nevertheless, how the introduction of a ligase reaction remodels the enthalpy-driven DNA circuit remains unclear. Herein, we systematically characterize kinetic profiles and background leakage behaviors of CHAC, confirming that T4 DNA ligase enhances overall product accumulation, with a more pronounced kinetic advantage at moderate to high target concentrations. Notably, the spontaneous upregulation of nonspecific leakage, typically induced alongside kinetic improvement, is effectively restricted in CHAC. Leveraging this enhanced kinetic performance with restricted background leakage, we further combined CHAC with nicking-enhanced rolling circle amplification (NickRCA) to construct an optomagnetic biosensor targeting the mecA gene in methicillin-resistant Staphylococcus aureus. CHAC-derived circular templates guide NickRCA to generate abundant short single-stranded amplicons, which cross-link detection probe-modified magnetic nanoparticles and elicit quantitative optomagnetic responses. This study characterizes ligase-dependent kinetic and leakage modulation of the present CHAC system, with the underlying mechanism proposed to involve both covalent cyclization and noncovalent duplex binding effects, which may be generalizable to other DNA strand displacement circuits.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03212","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T02:01:40Z","timestamp":1786154500000},"page":"24379-24387","source":"Crossref","is-referenced-by-count":2,"title":["Enzymatic Ligation-Enhanced Catalytic Hairpin Assembly\nfor Accelerated Kinetics with Restricted Leakage"],"prefix":"10.1021","volume":"98","author":[{"given":"Mengqing","family":"Sun","sequence":"first","affiliation":[{"name":"Central South University , , ,","place":["Changsha, China, 410013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Ma","sequence":"additional","affiliation":[{"name":"Central South University , , ,","place":["Changsha, China, 410013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huamin","family":"Yuan","sequence":"additional","affiliation":[{"name":"University of South China , , ,","place":["Changsha, China, 410007"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Su","sequence":"additional","affiliation":[{"name":"Central South University , , ,","place":["Changsha, China, 410013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Bi","sequence":"additional","affiliation":[{"name":"Central South University , , ,","place":["Changsha, China, 410013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheyuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Central South University , , ,","place":["Changsha, China, 410013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9059-2430","authenticated-orcid":true,"given":"Zhuxin","family":"Dong","sequence":"additional","affiliation":[{"name":"Central South University , , ,","place":["Changsha, China, 410013"]},{"name":"Furong Laboratory , ,","place":["Changsha, China, 410008"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoju","family":"Men","sequence":"additional","affiliation":[{"name":"Central South University , , ,","place":["Changsha, China, 410013"]},{"name":"Furong Laboratory , ,","place":["Changsha, China, 410008"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5249-4415","authenticated-orcid":true,"given":"Bo","family":"Tian","sequence":"additional","affiliation":[{"name":"Central South University , , ,","place":["Changsha, China, 410013"]},{"name":"Furong Laboratory , ,","place":["Changsha, China, 410008"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"issue":"28","key":"2026083118455598300_cit1","doi-asserted-by":"publisher","first-page":"5303","DOI":"10.1039\/D2TB00815G","article-title":"Recent Advances\nof Catalytic Hairpin Assembly and Its Application\nin Bioimaging and Biomedicine","volume":"10","author":"Tian","year":"2022","journal-title":"J. Mater. Chem.\nB"},{"issue":"7176","key":"2026083118455598300_cit2","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1038\/nature06451","article-title":"Programming Biomolecular\nSelf-Assembly Pathways","volume":"451","author":"Yin","year":"2008","journal-title":"Nature"},{"issue":"3","key":"2026083118455598300_cit3","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1007\/s00604-024-06947-4","article-title":"Terminal Deoxynucleotidyl\nTransferase\nCombined Dual-Toehold Catalytic Hairpin Assembly for Sensitive Sperm\nDNA Integrity Evaluation","volume":"192","author":"Pan","year":"2025","journal-title":"Microchim. Acta"},{"issue":"24","key":"2026083118455598300_cit4","doi-asserted-by":"publisher","first-page":"15953","DOI":"10.1021\/acs.analchem.0c03400","article-title":"Cascade\nCircuits on Self-Assembled DNA Polymers for\nTargeted RNA Imaging in Vivo","volume":"92","author":"Zhu","year":"2020","journal-title":"Anal. Chem."},{"key":"2026083118455598300_cit5","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.bios.2014.04.007","article-title":"Fast and Quantitative Differentiation of Single-Base\nMismatched DNA by Initial Reaction Rate of Catalytic Hairpin Assembly","volume":"60","author":"Li","year":"2014","journal-title":"Biosens. Bioelectron."},{"issue":"7","key":"2026083118455598300_cit6","doi-asserted-by":"publisher","first-page":"1845","DOI":"10.1002\/anie.201307418","article-title":"Mismatches\nImprove the Performance of Strand-Displacement Nucleic Acid Circuits","volume":"53","author":"Jiang","year":"2014","journal-title":"Angew. Chem., Int. Ed."},{"issue":"18","key":"2026083118455598300_cit7","doi-asserted-by":"publisher","first-page":"6363","DOI":"10.1021\/jacs.7b00530","article-title":"Multi-Arm Junctions\nfor Dynamic DNA Nanotechnology","volume":"139","author":"Kotani","year":"2017","journal-title":"J. Am. 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A."},{"issue":"10","key":"2026083118455598300_cit11","doi-asserted-by":"publisher","first-page":"1915","DOI":"10.1016\/j.bios.2006.01.014","article-title":"Polymerase\nChain Reaction\/Ligase\nDetection Reaction\/Hybridization Assays Using Flow-through Microfluidic\nDevices for the Detection of Low-Abundant DNA Point Mutations","volume":"21","author":"Hashimoto","year":"2006","journal-title":"Biosens. 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Bioelectron."},{"key":"2026083118455598300_cit14","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.talanta.2019.05.057","article-title":"Target-Catalyzed Hairpin\nStructure-Mediated Padlock Cyclization for Ultrasensitive Rolling\nCircle Amplification","volume":"204","author":"Song","year":"2019","journal-title":"Talanta"},{"issue":"51","key":"2026083118455598300_cit15","doi-asserted-by":"publisher","first-page":"44187","DOI":"10.1074\/jbc.M111.284992","article-title":"Kinetic\nCharacterization of Single\nStrand Break Ligation in Duplex DNA by T4 DNA Ligase","volume":"286","author":"Lohman","year":"2011","journal-title":"J. Biol. 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Acta"},{"issue":"1","key":"2026083118455598300_cit25","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1021\/acs.analchem.3c04533","article-title":"AND-Logic Cascade Rolling\nCircle Amplification for\nOptomagnetic Detection of Dual Target SARS-CoV-2 Sequences","volume":"96","author":"Ding","year":"2024","journal-title":"Anal. Chem."},{"issue":"15","key":"2026083118455598300_cit26","doi-asserted-by":"publisher","first-page":"10102","DOI":"10.1021\/acs.analchem.9b02073","article-title":"Ultrasensitive Real-Time Rolling Circle Amplification\nDetection Enhanced by Nicking-Induced Tandem-Acting Polymerases","volume":"91","author":"Tian","year":"2019","journal-title":"Anal. 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Lett."},{"issue":"W1","key":"2026083118455598300_cit29","doi-asserted-by":"publisher","first-page":"W491","DOI":"10.1093\/nar\/gkab324","article-title":"OxDNA.Org: A Public Webserver for Coarse-Grained Simulations\nof DNA and RNA Nanostructures","volume":"49","author":"Poppleton","year":"2021","journal-title":"Nucleic Acids\nRes."},{"issue":"12","key":"2026083118455598300_cit30","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa417","article-title":"Design, Optimization and Analysis of Large DNA and\nRNA Nanostructures through Interactive Visualization, Editing and\nMolecular Simulation","volume":"48","author":"Poppleton","year":"2020","journal-title":"Nucleic Acids Res."},{"issue":"23","key":"2026083118455598300_cit31","doi-asserted-by":"publisher","first-page":"2502062","DOI":"10.1002\/smll.202502062","article-title":"Ferromagnetic Resonance\nMultiplexing of Orthogonal Magnetic Nanoparticle Agglutination for\nSimultaneous Analysis of Nucleic Acid Mutations and Nonspecific Interactions","volume":"21","author":"Zhang","year":"2025","journal-title":"Small"},{"issue":"12","key":"2026083118455598300_cit32","doi-asserted-by":"publisher","first-page":"4792","DOI":"10.1021\/acssensors.3c01967","article-title":"On-Particle Hyperbranched\nRolling Circle Amplification-Scaffolded Magnetic Nanoactuator Assembly\nfor Ferromagnetic Resonance Detection of MicroRNA","volume":"8","author":"Fang","year":"2023","journal-title":"ACS Sens."},{"issue":"15","key":"2026083118455598300_cit33","doi-asserted-by":"publisher","first-page":"11104","DOI":"10.1021\/acs.analchem.5c07403","article-title":"Optomagnetic Viscometry for Monitoring Nanoscale Blood\nCoagulation\nDynamics In Vitro","volume":"98","author":"Bi","year":"2026","journal-title":"Anal. 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X"}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c03212","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/33\/24379\/66463458\/acs.analchem.6c03212.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/33\/24379\/66463458\/acs.analchem.6c03212.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T22:46:03Z","timestamp":1788216363000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/33\/24379\/5250604\/Enzymatic-Ligation-Enhanced-Catalytic-Hairpin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,7]]},"references-count":34,"journal-issue":{"issue":"33","published-online":{"date-parts":[[2026,8,7]]},"published-print":{"date-parts":[[2026,8,25]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c03212","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,7]]}}}