{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:19:08Z","timestamp":1788434348687,"version":"build-2803163510"},"reference-count":27,"publisher":"American Chemical Society (ACS)","issue":"34","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100005145","name":"Basic Research Program of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20252073"],"award-info":[{"award-number":["BK20252073"]}],"id":[{"id":"10.13039\/501100005145","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2632026XCZX01"],"award-info":[{"award-number":["2632026XCZX01"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2632026ZD06"],"award-info":[{"award-number":["2632026ZD06"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chemiluminescence (CL) imaging has been extensively investigated owing to its advantages, such as the lack of an external excitation source, low background signal, and high selectivity. Despite these advantages, existing CL probes for peroxynitrite (ONOO\u2013) detection are often hindered by complex synthetic routes and a propensity for false-positive signals. This study presents a novel CL probe DCM-C based on an \u03b1,\u03b1-dicyanoolefin skeleton to address these issues. DCM-C features a concise two-step synthesis and enables highly selective ONOO\u2013 detection with a superior signal-to-background ratio (SBR) of 552-fold. Both experimental and DFT theoretical calculation results indicate that CL depends on the \u03b1,\u03b1-dicyanoolefin skeleton through conical intersection. DCM-C is successfully used to image ONOO\u2013 in living cells with low cytotoxicity and monitor the ONOO\u2013 changes in a mouse model of ulcerative colitis (UC) with high contrast. This study provides an innovative strategy for developing high-sensitivity detection tools for ONOO\u2013 and has great potential for the early diagnosis and treatment processes of ulcerative colitis.<\/jats:p>","DOI":"10.1021\/acs.analchem.6c03230","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T01:01:16Z","timestamp":1787187676000},"page":"25174-25185","source":"Crossref","is-referenced-by-count":0,"title":["\u03b1,\u03b1-Dicyanoolefin-Based Chemiluminophore\nwith Conical Intersection for High-Contrast Imaging of Peroxynitrite\nin Ulcerative Colitis"],"prefix":"10.1021","volume":"98","author":[{"given":"Wenqing","family":"Li","sequence":"first","affiliation":[{"name":"Children\u2019s Hospital Affiliated to Zhengzhou University , , ,","place":["Zhengzhou, China, 450018"]},{"name":"China Pharmaceutical University , , ,","place":["Nanjing, China, 211198"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mu","family":"Kai","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology , , ,","place":["Luoyang, China, 471003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"China Pharmaceutical University , , ,","place":["Nanjing, China, 211198"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[{"name":"China Pharmaceutical University , , ,","place":["Nanjing, China, 211198"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijia","family":"Liu","sequence":"additional","affiliation":[{"name":"China Pharmaceutical University , , ,","place":["Nanjing, China, 211198"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5640-7067","authenticated-orcid":true,"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"China Pharmaceutical University , , ,","place":["Nanjing, China, 211198"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2359-908X","authenticated-orcid":true,"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"China Pharmaceutical University , , ,","place":["Nanjing, China, 211198"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3231-2602","authenticated-orcid":true,"given":"Wancun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Children\u2019s Hospital Affiliated to Zhengzhou University , , ,","place":["Zhengzhou, China, 450018"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"issue":"3","key":"2026090306473477900_cit1","doi-asserted-by":"publisher","first-page":"1338","DOI":"10.1021\/acs.chemrev.7b00568","article-title":"Biochemistry\nof peroxynitrite and protein tyrosine nitration","volume":"118","author":"Ferrer-Sueta","year":"2018","journal-title":"Chem. Rev."},{"issue":"13","key":"2026090306473477900_cit2","doi-asserted-by":"publisher","first-page":"5780","DOI":"10.1021\/acs.analchem.3c00142","article-title":"Multifunctional\nfluorescent probe for simultaneous detection of ONOO\u2013, viscosity,\nand polarity and its application in ferroptosis and cancer models","volume":"95","author":"Fan","year":"2023","journal-title":"Anal. Chem."},{"issue":"33","key":"2026090306473477900_cit3","doi-asserted-by":"publisher","first-page":"11728","DOI":"10.1021\/ja504624q","article-title":"Molecular imaging of\nperoxynitrite with HKGreen-4 in live cells and\ntissues","volume":"136","author":"Peng","year":"2014","journal-title":"J. Am. Chem. Soc."},{"issue":"9","key":"2026090306473477900_cit4","doi-asserted-by":"publisher","first-page":"1506","DOI":"10.1038\/sj.cdd.4401831","article-title":"Two distinct signaling\npathways regulate peroxynitrite-induced\napoptosis in PC12 cells","volume":"13","author":"Shacka","year":"2006","journal-title":"Cell Death Differ."},{"issue":"2","key":"2026090306473477900_cit5","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/S0027-5107(99)00221-3","article-title":"Peroxynitrite-induced DNA damage\nin the supF gene: correlation with the mutational spectrum","volume":"447","author":"Tretyakova","year":"2000","journal-title":"Mutat. Res., Fundam. Mol. Mech. Mutagen."},{"key":"2026090306473477900_cit6","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1016\/j.csbj.2015.03.004","article-title":"The role\nof excitotoxic programmed necrosis in acute brain injury","volume":"13","author":"Fujikawa","year":"2015","journal-title":"Comput. Struct. Biotechnol. J."},{"issue":"3","key":"2026090306473477900_cit7","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1007\/BF03346572","article-title":"Peroxynitrite-induced protein nitration\nis responsible for renal mitochondrial damage in diabetic rat","volume":"33","author":"Liang","year":"2010","journal-title":"J. Endocrinol. Invest."},{"key":"2026090306473477900_cit8","doi-asserted-by":"publisher","first-page":"340939","DOI":"10.1016\/j.aca.2023.340939","article-title":"Hyperbranched\npolysiloxane-based probe with enhanced\nlipophilicity for visualizing ONOO\u2013 fluctuations\nin endoplasmic reticulum","volume":"1249","author":"Zhang","year":"2023","journal-title":"Anal. Chim. 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Lett."}],"container-title":["Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.analchem.6c03230","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/34\/25174\/66886875\/acs.analchem.6c03230.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancham\/article-pdf\/98\/34\/25174\/66886875\/acs.analchem.6c03230.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:47:42Z","timestamp":1788432462000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancham\/article\/98\/34\/25174\/5277215\/Dicyanoolefin-Based-Chemiluminophore-with-Conical"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":27,"journal-issue":{"issue":"34","published-online":{"date-parts":[[2026,8,19]]},"published-print":{"date-parts":[[2026,9,1]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.analchem.6c03230","relation":{},"ISSN":["0003-2700","1520-6882"],"issn-type":[{"value":"0003-2700","type":"print"},{"value":"1520-6882","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]}}}